1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	A	P	V	R	Q	R	S	H	D	R	P	A	S	L	D	N	P	R	S	P	R	R	R	A	G	M	P	N	F	E	K	F	A	W	L	F	M	R	F	S	G	V	V	L	V	F	L	A	I	G	H	V	F	I	M	L	M	W
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	N	G	V	Y	R	L	D	F	N	F	V	A	Q	R	W	A	S	P	F	W	Q	T	W	D	L	L	L	L	W	L	A	Q	L	H	G	G	N	G	L	R	T	I	I	D	D	Y	S	R	K	D	T	T	R	F	W	L	N	S	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.
Sequence		L	V	L	S	M	L	F	T	L	M	L	G	T	Y	V	I	V	T	F	D	P	N	I	S
Secondary structure
SS confidence
Disorder																						?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 24%) Alpha helix ( 73%) Beta strand ( 0%) TM helix ( 46%) (View membrane topology prediction)

Rank Aligned region

PDB 1nek chain D Region: 27 - 137 Aligned: 107 Modelled: 111 Confidence: 99.9% Identity: 20% Fold: Heme-binding four-helical bundle Superfamily: Fumarate reductase respiratory complex transmembrane subunits Family: Succinate dehydrogenase/Fumarate reductase transmembrane subunits (SdhC/FrdC and SdhD/FrdD) Phyre2 PDB 2bs2 chain C domain 1 Region: 13 - 139 Aligned: 124 Modelled: 127 Confidence: 99.8% Identity: 23% Fold: Heme-binding four-helical bundle Superfamily: Fumarate reductase respiratory complex transmembrane subunits Family: Fumarate reductase respiratory complex cytochrome b subunit, FrdC Phyre2 PDB 5xmj chain G Region: 13 - 139 Aligned: 122 Modelled: 127 Confidence: 99.6% Identity: 25% PDB header:electron transport Chain: G: PDB Molecule:fumarate reductase respiratory complex; PDBTitle: crystal structure of quinol:fumarate reductase from desulfovibrio2 gigas Phyre2 PDB 1nek chain C Region: 12 - 137 Aligned: 124 Modelled: 126 Confidence: 99.4% Identity: 16% Fold: Heme-binding four-helical bundle Superfamily: Fumarate reductase respiratory complex transmembrane subunits Family: Succinate dehydrogenase/Fumarate reductase transmembrane subunits (SdhC/FrdC and SdhD/FrdD) Phyre2 PDB 1yq3 chain C Region: 5 - 134 Aligned: 128 Modelled: 130 Confidence: 99.1% Identity: 16% PDB header:oxidoreductase Chain: C: PDB Molecule:succinate dehydrogenase cytochrome b, large subunit; PDBTitle: avian respiratory complex ii with oxaloacetate and ubiquinone Phyre2 PDB 3vrb chain G Region: 19 - 130 Aligned: 110 Modelled: 112 Confidence: 99.0% Identity: 15% PDB header:oxidoreductase/oxidoreductase inhibitor Chain: G: PDB Molecule:cytochrome b-large subunit; PDBTitle: mitochondrial rhodoquinol-fumarate reductase from the parasitic2 nematode ascaris suum with the specific inhibitor flutolanil and3 substrate fumarate Phyre2 PDB 1zp0 chain D Region: 29 - 143 Aligned: 91 Modelled: 97 Confidence: 98.3% Identity: 25% PDB header:oxidoreductase Chain: D: PDB Molecule:small cytochrome binding protein; PDBTitle: crystal structure of mitochondrial respiratory complex ii2 bound with 3-nitropropionate and 2-thenoyltrifluoroacetone Phyre2 PDB 3vr8 chain H Region: 10 - 140 Aligned: 107 Modelled: 108 Confidence: 98.3% Identity: 17% PDB header:oxidoreductase Chain: H: PDB Molecule:succinate dehydrogenase [ubiquinone] cytochrome b small PDBTitle: mitochondrial rhodoquinol-fumarate reductase from the parasitic2 nematode ascaris suum Phyre2 PDB 1kf6 chain C Region: 17 - 136 Aligned: 118 Modelled: 120 Confidence: 96.5% Identity: 18% Fold: Heme-binding four-helical bundle Superfamily: Fumarate reductase respiratory complex transmembrane subunits Family: Succinate dehydrogenase/Fumarate reductase transmembrane subunits (SdhC/FrdC and SdhD/FrdD) Phyre2 PDB 1kf6 chain D Region: 16 - 138 Aligned: 114 Modelled: 123 Confidence: 95.9% Identity: 16% Fold: Heme-binding four-helical bundle Superfamily: Fumarate reductase respiratory complex transmembrane subunits Family: Succinate dehydrogenase/Fumarate reductase transmembrane subunits (SdhC/FrdC and SdhD/FrdD) Phyre2 PDB 6hrb chain D Region: 41 - 59 Aligned: 19 Modelled: 19 Confidence: 27.5% Identity: 42% PDB header:membrane protein Chain: D: PDB Molecule:potassium-transporting atpase kdpf subunit; PDBTitle: cryo-em structure of the kdpfabc complex in an e2 inward-facing state2 (state 2) Phyre2 PDB 5mrw chain D Region: 41 - 59 Aligned: 19 Modelled: 19 Confidence: 27.5% Identity: 42% PDB header:hydrolase Chain: D: PDB Molecule:potassium-transporting atpase kdpf subunit; PDBTitle: structure of the kdpfabc complex Phyre2 PDB 6hra chain D Region: 41 - 59 Aligned: 19 Modelled: 19 Confidence: 27.5% Identity: 42% PDB header:membrane protein Chain: D: PDB Molecule:potassium-transporting atpase kdpf subunit; PDBTitle: cryo-em structure of the kdpfabc complex in an e1 outward-facing state2 (state 1) Phyre2 PDB 5mrw chain L Region: 41 - 59 Aligned: 19 Modelled: 19 Confidence: 27.5% Identity: 42% PDB header:hydrolase Chain: L: PDB Molecule:potassium-transporting atpase kdpf subunit; PDBTitle: structure of the kdpfabc complex Phyre2 PDB 5mrw chain H Region: 41 - 59 Aligned: 19 Modelled: 19 Confidence: 27.5% Identity: 42% PDB header:hydrolase Chain: H: PDB Molecule:potassium-transporting atpase kdpf subunit; PDBTitle: structure of the kdpfabc complex Phyre2 PDB 3izb chain E Region: 1 - 37 Aligned: 36 Modelled: 37 Confidence: 14.2% Identity: 31% PDB header:ribosome Chain: E: PDB Molecule:40s ribosomal protein rps2 (s5p); PDBTitle: localization of the small subunit ribosomal proteins into a 6.1 a2 cryo-em map of saccharomyces cerevisiae translating 80s ribosome Phyre2 PDB 5vkv chain A Region: 111 - 144 Aligned: 34 Modelled: 34 Confidence: 13.4% Identity: 9% PDB header:oxidoreductase Chain: A: PDB Molecule:cytochrome c-type biogenesis protein ccda; PDBTitle: solution nmr structure of the membrane electron transporter ccda Phyre2 PDB 5o0t chain A Region: 37 - 94 Aligned: 55 Modelled: 58 Confidence: 9.3% Identity: 22% PDB header:membrane protein Chain: A: PDB Molecule:putative ferric reductase; PDBTitle: crystal structure of trans-membrane domain of cylindrospermum stagnale2 nadph-oxidase 5 (nox5) Phyre2 PDB 6btm chain C Region: 2 - 137 Aligned: 126 Modelled: 136 Confidence: 7.4% Identity: 12% PDB header:membrane protein Chain: C: PDB Molecule:alternative complex iii subunit c; PDBTitle: structure of alternative complex iii from flavobacterium johnsoniae2 (wild type) Phyre2 PDB 2zxe chain G Region: 117 - 138 Aligned: 22 Modelled: 22 Confidence: 7.0% Identity: 9% PDB header:hydrolase/transport protein Chain: G: PDB Molecule:phospholemman-like protein; PDBTitle: crystal structure of the sodium - potassium pump in the e2.2k+.pi2 state Phyre2 1 d1nekd_ 2 d2bs2c1 3 c5xmjG_ 4 d1nekc_ 5 c1yq3C_ 6 c3vrbG_ 7 c1zp0D_ 8 c3vr8H_ 9 d1kf6c_ 10 d1kf6d_ 11 c6hrbD_ 12 c5mrwD_ 13 c6hraD_ 14 c5mrwL_ 15 c5mrwH_ 16 c3izbE_ 17 c5vkvA_ 18 c5o0tA_ 19 c6btmC_ 20 c2zxeG_ 21 22 23 24 25 26 27 28