1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	F	L	R	S	V	S	C	L	A	A	A	V	F	A	V	G	T	G	I	G	L	P	T	A	A	G	E	P	N	A	A	P	A	A	C	P	Y	K	V	S	T	P	P	A	V	D	S	S	E	V	P	A	A	G	E	P	P	L
Secondary structure
SS confidence
Disorder		?	?																?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		P	L	V	V	P	P	T	P	V	G	G	N	A	L	G	G	C	G	I	I	T	A	P	G	S	A	P	A	P	G	D	V	S	A	E	A	W	L	V	A	D	L	D	S	G	A	V	I	A	A	R	D	P	H	G	R	H	R	P	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		S	V	I	K	V	L	V	A	M	A	S	I	N	T	L	T	L	N	K	S	V	A	G	T	A	D	D	A	A	V	E	G	T	K	V	G	V	N	T	G	G	T	Y	T	V	N	Q	L	L	H	G	L	L	M	H	S	G	N	D	A
Secondary structure
SS confidence
Disorder																															?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	Y	A	L	A	R	Q	L	G	G	M	P	A	A	L	E	K	I	N	L	L	A	A	K	L	G	G	R	D	T	R	V	A	T	P	S	G	L	D	G	P	G	M	S	T	S	A	Y	D	I	G	L	F	Y	R	Y	A	W	Q	N
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		P	V	F	A	D	I	V	A	T	R	T	F	D	F	P	G	H	G	D	H	P	G	Y	E	L	E	N	D	N	Q	L	L	Y	N	Y	P	G	A	L	G	G	K	T	G	Y	T	D	D	A	G	Q	T	F	V	G	A	A	N	R	D
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		G	R	R	L	M	T	V	L	L	H	G	T	R	Q	P	I	P	P	W	E	Q	A	A	H	L	L	D	Y	G	F	N	T	P	A	G	T	Q	I	G	T	L	I	E	P	D	P	S	L	M	S	T	D	R	N	P	A	D	R	Q	R
Secondary structure
SS confidence
Disorder																?		?																				?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.
Sequence		V	D	P	Q	A	A	A	R	I	S	A	A	D	A	L	P	V	R	V	G	V	A	V	I	G	A	L	I	V	F	G	L	I	M	V	A	R	A	M	N	R	R	P	Q	H
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?									?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 35%) Alpha helix ( 36%) Beta strand ( 15%) TM helix ( 4%) (View membrane topology prediction)

Rank Aligned region

PDB 4ppr chain A Region: 59 - 358 Aligned: 300 Modelled: 300 Confidence: 100.0% Identity: 98% PDB header:hydrolase Chain: A: PDB Molecule:penicillin-binding protein dacb1; PDBTitle: crystal structure of mycobacterium tuberculosis d,d-peptidase rv33302 in complex with meropenem Phyre2 PDB 6osu chain A Region: 70 - 349 Aligned: 275 Modelled: 280 Confidence: 100.0% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:d-alanyl-d-alanine carboxypeptidase (penicillin binding PDBTitle: crystal structure of the d-alanyl-d-alanine carboxypeptidase dacd from2 francisella tularensis Phyre2 PDB 4k91 chain A Region: 87 - 359 Aligned: 266 Modelled: 267 Confidence: 100.0% Identity: 27% PDB header:hydrolase Chain: A: PDB Molecule:d-ala-d-ala-carboxypeptidase; PDBTitle: crystal structure of penicillin-binding protein 5 (pbp5) from2 pseudomonas aeruginosa in apo state Phyre2 PDB 3mfd chain B Region: 91 - 338 Aligned: 240 Modelled: 248 Confidence: 100.0% Identity: 32% PDB header:hydrolase Chain: B: PDB Molecule:d-alanyl-d-alanine carboxypeptidase dacb; PDBTitle: the structure of the beta-lactamase superfamily domain of d-alanyl-d-2 alanine carboxypeptidase from bacillus subtilis Phyre2 PDB 1z6f chain A Region: 83 - 359 Aligned: 270 Modelled: 277 Confidence: 100.0% Identity: 26% PDB header:hydrolase Chain: A: PDB Molecule:penicillin-binding protein 5; PDBTitle: crystal structure of penicillin-binding protein 5 from e. coli in2 complex with a boronic acid inhibitor Phyre2 PDB 3it9 chain B Region: 90 - 359 Aligned: 263 Modelled: 270 Confidence: 100.0% Identity: 27% PDB header:hydrolase Chain: B: PDB Molecule:d-alanyl-d-alanine carboxypeptidase dacc; PDBTitle: crystal structure of penicillin-binding protein 6 (pbp6) from e. coli2 in apo state Phyre2 PDB 5fsr chain B Region: 85 - 344 Aligned: 253 Modelled: 260 Confidence: 100.0% Identity: 30% PDB header:hydrolase Chain: B: PDB Molecule:d-alanyl-d-alanine carboxypeptidase dacd; PDBTitle: crystal structure of penicillin binding protein 6b from2 escherichia coli Phyre2 PDB 3a3j chain A Region: 88 - 359 Aligned: 265 Modelled: 272 Confidence: 100.0% Identity: 25% PDB header:hydrolase Chain: A: PDB Molecule:pbp5; PDBTitle: crystal structures of penicillin binding protein 5 from haemophilus2 influenzae Phyre2 PDB 1xp4 chain C Region: 91 - 344 Aligned: 248 Modelled: 254 Confidence: 100.0% Identity: 26% PDB header:hydrolase Chain: C: PDB Molecule:d-alanyl-d-alanine carboxypeptidase; PDBTitle: crystal structure of a peptidoglycan synthesis regulatory2 factor (pbp3) from streptococcus pneumoniae Phyre2 PDB 1tvf chain A Region: 90 - 338 Aligned: 243 Modelled: 249 Confidence: 100.0% Identity: 19% PDB header:penicillin binding Chain: A: PDB Molecule:penicillin binding protein 4; PDBTitle: crystal structure of penicillin-binding protein 4 (pbp4)2 from staphylococcus aureus Phyre2 PDB 3hum chain B Region: 83 - 344 Aligned: 255 Modelled: 262 Confidence: 100.0% Identity: 20% PDB header:hydrolase/antibiotics Chain: B: PDB Molecule:penicillin-binding protein 4; PDBTitle: crystal structure of penicillin binding protein 4 from staphylococcus2 aureus col in complex with cefotaxime Phyre2 PDB 2bcf chain A Region: 81 - 341 Aligned: 257 Modelled: 261 Confidence: 100.0% Identity: 47% PDB header:hydrolase Chain: A: PDB Molecule:probable d-alanyl-d-alanine carboxypeptidase dacb2; PDBTitle: crystal structure of a evolved putative penicillin-binding protein2 homolog, rv2911, from mycobacterium tuberculosis. Phyre2 PDB 6azi chain A Region: 90 - 339 Aligned: 246 Modelled: 246 Confidence: 100.0% Identity: 22% PDB header:hydrolase Chain: A: PDB Molecule:d-alanyl-d-alanine endopeptidase; PDBTitle: 1.75 angstrom resolution crystal structure of d-alanyl-d-alanine2 endopeptidase from enterobacter cloacae in complex with covalently3 bound boronic acid Phyre2 PDB 1tvf chain A domain 2 Region: 84 - 333 Aligned: 243 Modelled: 250 Confidence: 100.0% Identity: 20% Fold: beta-lactamase/transpeptidase-like Superfamily: beta-lactamase/transpeptidase-like Family: beta-Lactamase/D-ala carboxypeptidase Phyre2 PDB 3bec chain A domain 2 Region: 83 - 331 Aligned: 242 Modelled: 249 Confidence: 100.0% Identity: 29% Fold: beta-lactamase/transpeptidase-like Superfamily: beta-lactamase/transpeptidase-like Family: beta-Lactamase/D-ala carboxypeptidase Phyre2 PDB 1es5 chain A Region: 90 - 331 Aligned: 241 Modelled: 242 Confidence: 100.0% Identity: 27% Fold: beta-lactamase/transpeptidase-like Superfamily: beta-lactamase/transpeptidase-like Family: beta-Lactamase/D-ala carboxypeptidase Phyre2 PDB 1xp4 chain A domain 2 Region: 91 - 333 Aligned: 237 Modelled: 243 Confidence: 100.0% Identity: 27% Fold: beta-lactamase/transpeptidase-like Superfamily: beta-lactamase/transpeptidase-like Family: beta-Lactamase/D-ala carboxypeptidase Phyre2 PDB 5tr7 chain A Region: 89 - 334 Aligned: 239 Modelled: 246 Confidence: 100.0% Identity: 28% PDB header:penicillin-binding protein Chain: A: PDB Molecule:d-alanyl-d-alanine carboxypeptidase; PDBTitle: crystal structure of a putative d-alanyl-d-alanine carboxypeptidase2 from vibrio cholerae o1 biovar eltor str. n16961 Phyre2 PDB 5zqe chain C Region: 88 - 331 Aligned: 237 Modelled: 244 Confidence: 100.0% Identity: 28% PDB header:antibiotic Chain: C: PDB Molecule:lmo2812 protein; PDBTitle: crystal structure of penicillin-binding protein d2 from listeria2 monocytogenes in the cefuroxime bound form Phyre2 PDB 3cg5 chain A Region: 96 - 326 Aligned: 226 Modelled: 227 Confidence: 100.0% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:beta-lactamase; PDBTitle: crystal structure of the covalent adduct formed between tb2 b-lactamase and clavulanate Phyre2 1 c4pprA_ 2 c6osuA_ 3 c4k91A_ 4 c3mfdB_ 5 c1z6fA_ 6 c3it9B_ 7 c5fsrB_ 8 c3a3jA_ 9 c1xp4C_ 10 c1tvfA_ 11 c3humB_ 12 c2bcfA_ 13 c6aziA_ 14 d1tvfa2 15 d3beca2 16 d1es5a_ 17 d1xp4a2 18 c5tr7A_ 19 c5zqeC_ 20 c3cg5A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186