1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	R	R	R	A	M	T	K	M	D	E	A	S	N	P	C	G	G	D	I	E	A	E	M	C	Q	L	M	R	E	Q	P	P	A	E	G	V	V	D	R	V	A	L	Q	R	H	R	N	V	A	L	I	T	L	S	H	P	Q	A	Q
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		N	A	L	N	L	A	S	W	R	R	L	K	R	L	L	D	D	L	A	G	E	S	G	L	R	A	V	V	L	R	G	A	G	D	K	A	F	A	A	G	A	D	I	K	E	F	P	N	T	R	M	S	A	A	D	A	A	E	Y	N
Secondary structure
SS confidence
Disorder																																																?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		E	S	L	A	V	C	L	R	A	L	T	T	M	P	I	P	V	I	A	A	V	R	G	L	A	V	G	G	G	C	E	L	A	T	A	C	D	V	C	I	A	T	D	D	A	R	F	G	I	P	L	G	K	L	G	V	T	T	G	F
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.
Sequence		T	E	A	D	T	V	A	R	L	I	G	P	A	A	L	K	Y	L	L	F	S	G	E	L	I	G	I	E	E	A	A	R	W
Secondary structure
SS confidence
Disorder																																?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 21%) Alpha helix ( 41%) Beta strand ( 15%) TM helix ( 9%) (View membrane topology prediction)

Rank Aligned region

PDB 4k2n chain A Region: 37 - 213 Aligned: 177 Modelled: 175 Confidence: 100.0% Identity: 38% PDB header:isomerase Chain: A: PDB Molecule:enoyl-coa hydratase/carnithine racemase; PDBTitle: crystal structure of an enoyl-coa hydratase/ carnithine racemase from2 magnetospirillum magneticum Phyre2 PDB 5jbx chain B Region: 36 - 213 Aligned: 176 Modelled: 178 Confidence: 100.0% Identity: 32% PDB header:lyase Chain: B: PDB Molecule:3-hydroxybutyryl-coa dehydratase; PDBTitle: crystal structure of liuc in complex with coenzyme a and malonic acid Phyre2 PDB 3t88 chain A Region: 32 - 213 Aligned: 182 Modelled: 182 Confidence: 100.0% Identity: 27% PDB header:lyase/lyase inhibitor Chain: A: PDB Molecule:1,4-dihydroxy-2-naphthoyl-coa synthase; PDBTitle: crystal structure of escherichia coli menb in complex with substrate2 analogue, osb-ncoa Phyre2 PDB 5z7r chain A Region: 37 - 213 Aligned: 175 Modelled: 177 Confidence: 100.0% Identity: 38% PDB header:lyase Chain: A: PDB Molecule:short-chain-enoyl-coa hydratase; PDBTitle: crystal strcuture of crotonase from clostridium acetobutylicum Phyre2 PDB 3g64 chain A Region: 27 - 213 Aligned: 187 Modelled: 187 Confidence: 100.0% Identity: 28% PDB header:lyase Chain: A: PDB Molecule:putative enoyl-coa hydratase; PDBTitle: crystal structure of putative enoyl-coa hydratase from streptomyces2 coelicolor a3(2) Phyre2 PDB 4hdt chain A Region: 38 - 213 Aligned: 175 Modelled: 176 Confidence: 100.0% Identity: 21% PDB header:isomerase Chain: A: PDB Molecule:3-hydroxyisobutyryl-coa hydrolase; PDBTitle: crystal structure of a carnitinyl-coa dehydratase from mycobacterium2 thermoresistibile Phyre2 PDB 4j2u chain A Region: 35 - 213 Aligned: 178 Modelled: 179 Confidence: 100.0% Identity: 24% PDB header:lyase Chain: A: PDB Molecule:enoyl-coa hydratase; PDBTitle: crystal structure of an enoyl-coa hydratase from rhodobacter2 sphaeroides 2.4.1 Phyre2 PDB 4olq chain D Region: 41 - 213 Aligned: 173 Modelled: 173 Confidence: 100.0% Identity: 32% PDB header:lyase Chain: D: PDB Molecule:enoyl-coa hydratase/isomerase family protein; PDBTitle: crystal structure of a putative enoyl-coa hydratase/isomerase family2 protein from hyphomonas neptunium Phyre2 PDB 3lke chain A Region: 39 - 213 Aligned: 175 Modelled: 175 Confidence: 100.0% Identity: 25% PDB header:lyase Chain: A: PDB Molecule:enoyl-coa hydratase; PDBTitle: crystal structure of enoyl-coa hydratase from bacillus halodurans Phyre2 PDB 3rrv chain C Region: 38 - 213 Aligned: 175 Modelled: 176 Confidence: 100.0% Identity: 25% PDB header:isomerase Chain: C: PDB Molecule:enoyl-coa hydratase/isomerase; PDBTitle: crystal structure of an enoyl-coa hydratase/isomerase from2 mycobacterium paratuberculosis Phyre2 PDB 3bpt chain A Region: 35 - 213 Aligned: 178 Modelled: 179 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:3-hydroxyisobutyryl-coa hydrolase; PDBTitle: crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in2 complex with quercetin Phyre2 PDB 2w3p chain B Region: 33 - 213 Aligned: 181 Modelled: 181 Confidence: 100.0% Identity: 24% PDB header:lyase Chain: B: PDB Molecule:benzoyl-coa-dihydrodiol lyase; PDBTitle: boxc crystal structure Phyre2 PDB 4jcs chain A Region: 37 - 213 Aligned: 175 Modelled: 175 Confidence: 100.0% Identity: 23% PDB header:lyase Chain: A: PDB Molecule:enoyl-coa hydratase/isomerase; PDBTitle: crystal structure of enoyl-coa hydratase/isomerase from cupriavidus2 metallidurans ch34 Phyre2 PDB 4wcz chain B Region: 39 - 213 Aligned: 175 Modelled: 174 Confidence: 100.0% Identity: 35% PDB header:isomerase Chain: B: PDB Molecule:enoyl-coa hydratase/isomerase; PDBTitle: crystal structure of a putative enoyl-coa hydratase/isomerase from2 novosphingobium aromaticivorans Phyre2 PDB 1pjh chain A Region: 36 - 213 Aligned: 178 Modelled: 178 Confidence: 100.0% Identity: 14% Fold: ClpP/crotonase Superfamily: ClpP/crotonase Family: Crotonase-like Phyre2 PDB 4izb chain B Region: 35 - 213 Aligned: 177 Modelled: 179 Confidence: 100.0% Identity: 31% PDB header:hydrolase Chain: B: PDB Molecule:enoyl-coa hydratase/isomerase family protein; PDBTitle: crystal structure of dmdd, a crotonase superfamily enzyme that2 catalyzes the hydration and hydrolysis of methylthioacryloyl-coa Phyre2 PDB 1nzy chain A Region: 38 - 213 Aligned: 175 Modelled: 176 Confidence: 100.0% Identity: 25% Fold: ClpP/crotonase Superfamily: ClpP/crotonase Family: Crotonase-like Phyre2 PDB 5zai chain B Region: 37 - 213 Aligned: 174 Modelled: 176 Confidence: 100.0% Identity: 31% PDB header:hydrolase Chain: B: PDB Molecule:3-hydroxypropionyl-coenzyme a dehydratase; PDBTitle: crystal strcuture of 3-hydroxypropionyl-coa dehydratase from2 metallosphaera sedula Phyre2 PDB 3i47 chain A Region: 37 - 213 Aligned: 175 Modelled: 177 Confidence: 100.0% Identity: 30% PDB header:lyase Chain: A: PDB Molecule:enoyl coa hydratase/isomerase (crotonase); PDBTitle: crystal structure of putative enoyl coa hydratase/isomerase2 (crotonase) from legionella pneumophila subsp. pneumophila str.3 philadelphia 1 Phyre2 PDB 4zu2 chain A Region: 39 - 213 Aligned: 174 Modelled: 175 Confidence: 100.0% Identity: 30% PDB header:hydrolase Chain: A: PDB Molecule:putative isohexenylglutaconyl-coa hydratase; PDBTitle: pseudomonas aeruginosa atue Phyre2 1 c4k2nA_ 2 c5jbxB_ 3 c3t88A_ 4 c5z7rA_ 5 c3g64A_ 6 c4hdtA_ 7 c4j2uA_ 8 c4olqD_ 9 c3lkeA_ 10 c3rrvC_ 11 c3bptA_ 12 c2w3pB_ 13 c4jcsA_ 14 c4wczB_ 15 d1pjha_ 16 c4izbB_ 17 d1nzya_ 18 c5zaiB_ 19 c3i47A_ 20 c4zu2A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195