1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	P	S	P	A	T	T	W	L	H	V	S	G	Y	R	F	L	L	R	R	I	E	C	A	L	L	F	G	D	V	C	A	A	T	G	A	L	R	A	R	T	T	S	L	A	L	G	C	V	L	A	I	V	A	A	M	G	C	A	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																?	?			?		?		?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		V	A	L	L	R	P	Q	S	A	L	G	Q	A	P	I	V	M	G	R	E	S	G	A	L	Y	V	R	V	D	D	V	W	H	P	V	L	N	L	A	S	A	R	L	I	A	A	T	N	A	N	P	Q	P	V	S	E	S	E	L	G
Secondary structure
SS confidence
Disorder												?	?																																			?	?	?	?	?			?						?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		H	T	K	R	G	P	L	L	G	I	P	G	A	P	Q	L	L	D	Q	P	L	A	G	A	E	S	A	W	A	I	C	D	S	D	N	G	G	S	T	T	V	V	V	G	P	A	E	D	S	S	A	Q	V	L	T	A	E	Q	M	I
Secondary structure
SS confidence
Disorder		?	?		?															?	?	?	?	?	?	?	?							?	?	?	?	?	?						?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	V	A	T	E	S	G	S	P	T	Y	L	L	Y	G	G	R	R	A	V	V	D	L	A	D	P	A	V	V	W	A	L	R	L	Q	G	R	V	P	H	V	V	A	Q	S	L	L	N	A	V	P	E	A	P	R	I	T	A	P	R
Secondary structure
SS confidence
Disorder																									?	?	?								?	?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		I	R	G	G	G	R	A	S	V	G	L	P	G	F	L	V	G	G	V	V	R	I	T	R	A	S	G	D	E	Y	Y	V	V	L	E	D	G	V	Q	R	I	G	Q	V	A	A	D	L	L	R	F	G	D	S	Q	G	S	V	N	V
Secondary structure
SS confidence
Disorder			?	?	?	?	?	?	?	?	?	?	?																										?																?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		P	T	V	A	P	D	V	I	R	V	A	P	I	V	N	T	L	P	V	S	A	F	P	D	R	P	P	T	P	V	D	G	S	P	G	R	A	V	T	T	L	C	V	T	W	T	P	A	Q	P	G	A	A	R	V	A	F	L	A	G
Secondary structure
SS confidence
Disorder		?	?	?	?					?	?	?	?	?	?	?	?	?	?	?	?	?	?							?	?	?	?	?	?	?	?	?													?		?							?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		S	G	P	P	V	P	L	G	G	V	P	V	T	L	A	Q	A	D	G	R	G	P	A	L	D	A	V	Y	L	P	P	G	R	S	A	Y	V	A	A	R	S	L	S	G	G	G	T	G	T	R	Y	L	V	T	D	T	G	V	R	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?																	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470
Sequence		A	I	H	D	D	D	V	A	H	D	L	G	L	P	T	A	A	I	P	A	P	W	P	V	L	A	T	L	P	S	G	P	E	L	S	R	A	N	A	S	V	A	R	D	T	V	A	P	G	P
Secondary structure
SS confidence
Disorder																	?														?			?									?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 35%) Alpha helix ( 22%) Beta strand ( 20%) TM helix ( 4%) (View membrane topology prediction)

Rank Aligned region

PDB 4kk7 chain A Region: 74 - 461 Aligned: 373 Modelled: 388 Confidence: 100.0% Identity: 30% PDB header:protein transport Chain: A: PDB Molecule:esx-1 secretion system protein eccb1; PDBTitle: structure of eccb1 from the type vii (esx-1) secretion system of2 mycobacterium tuberculosis. Phyre2 PDB 5cyu chain A Region: 74 - 461 Aligned: 366 Modelled: 386 Confidence: 100.0% Identity: 33% PDB header:membrane protein Chain: A: PDB Molecule:conserved membrane protein; PDBTitle: structure of the soluble domain of eccb1 from the mycobacterium2 smegmatis esx-1 secretion system. Phyre2 PDB 6nav chain I Region: 44 - 89 Aligned: 46 Modelled: 46 Confidence: 38.1% Identity: 15% PDB header:structural protein Chain: I: PDB Molecule:m9ud72; PDBTitle: cryo-em reconstruction of sulfolobus islandicus lal14/1 pilus Phyre2 PDB 2fb5 chain A domain 1 Region: 75 - 95 Aligned: 21 Modelled: 21 Confidence: 33.0% Identity: 14% Fold: YojJ-like Superfamily: YojJ-like Family: YojJ-like Phyre2 PDB 2i5n chain H domain 1 Region: 75 - 124 Aligned: 46 Modelled: 50 Confidence: 30.5% Identity: 11% Fold: PRC-barrel domain Superfamily: PRC-barrel domain Family: Photosynthetic reaction centre, H-chain, cytoplasmic domain Phyre2 PDB 1k6n chain H Region: 75 - 124 Aligned: 46 Modelled: 50 Confidence: 29.4% Identity: 13% PDB header:photosynthesis Chain: H: PDB Molecule:photosynthetic reaction center h subunit; PDBTitle: e(l212)a,d(l213)a double mutant structure of photosynthetic reaction2 center from rhodobacter sphaeroides Phyre2 PDB 2i5n chain H Region: 75 - 124 Aligned: 46 Modelled: 50 Confidence: 24.3% Identity: 11% PDB header:photosynthesis Chain: H: PDB Molecule:reaction center protein h chain; PDBTitle: 1.96 a x-ray structure of photosynthetic reaction center from2 rhodopseudomonas viridis:crystals grown by microfluidic technique Phyre2 PDB 5xef chain A Region: 14 - 35 Aligned: 22 Modelled: 22 Confidence: 20.0% Identity: 14% PDB header:chaperone Chain: A: PDB Molecule:flagellar protein flis; PDBTitle: crystal structure of flagellar chaperone from bacteria Phyre2 PDB 1rzh chain H domain 1 Region: 75 - 124 Aligned: 46 Modelled: 50 Confidence: 16.1% Identity: 13% Fold: PRC-barrel domain Superfamily: PRC-barrel domain Family: Photosynthetic reaction centre, H-chain, cytoplasmic domain Phyre2 PDB 2k9y chain B Region: 35 - 64 Aligned: 30 Modelled: 30 Confidence: 15.8% Identity: 13% PDB header:transferase Chain: B: PDB Molecule:ephrin type-a receptor 2; PDBTitle: epha2 dimeric structure in the lipidic bicelle at ph 5.0 Phyre2 PDB 2k9y chain A Region: 35 - 64 Aligned: 30 Modelled: 30 Confidence: 15.8% Identity: 13% PDB header:transferase Chain: A: PDB Molecule:ephrin type-a receptor 2; PDBTitle: epha2 dimeric structure in the lipidic bicelle at ph 5.0 Phyre2 PDB 1vh6 chain A Region: 14 - 34 Aligned: 21 Modelled: 18 Confidence: 14.7% Identity: 24% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:flagellar protein flis; PDBTitle: crystal structure of a flagellar protein Phyre2 PDB 1vh6 chain A Region: 14 - 34 Aligned: 21 Modelled: 18 Confidence: 14.7% Identity: 24% Fold: Four-helical up-and-down bundle Superfamily: Flagellar export chaperone FliS Family: Flagellar export chaperone FliS Phyre2 PDB 3k1i chain A Region: 14 - 35 Aligned: 22 Modelled: 22 Confidence: 13.8% Identity: 14% PDB header:chaperone Chain: A: PDB Molecule:flagellar protein; PDBTitle: crystal strcture of flis-hp1076 complex in h. pylori Phyre2 PDB 2ciu chain A Region: 75 - 97 Aligned: 23 Modelled: 23 Confidence: 11.2% Identity: 22% PDB header:protein transport Chain: A: PDB Molecule:import inner membrane translocase subunit tim21 PDBTitle: structure of the ims domain of the mitochondrial import2 protein tim21 from s. cerevisiae Phyre2 PDB 6f0k chain A Region: 39 - 75 Aligned: 36 Modelled: 37 Confidence: 11.1% Identity: 14% PDB header:membrane protein Chain: A: PDB Molecule:cytochrome c family protein; PDBTitle: alternative complex iii Phyre2 PDB 6cfw chain E Region: 50 - 66 Aligned: 17 Modelled: 17 Confidence: 9.4% Identity: 35% PDB header:membrane protein Chain: E: PDB Molecule:mbh subunit; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 2wmm chain A Region: 8 - 53 Aligned: 44 Modelled: 46 Confidence: 8.6% Identity: 9% PDB header:cell cycle Chain: A: PDB Molecule:chromosome partition protein mukb; PDBTitle: crystal structure of the hinge domain of mukb Phyre2 PDB 6ch3 chain B Region: 14 - 35 Aligned: 22 Modelled: 22 Confidence: 8.3% Identity: 32% PDB header:structural protein Chain: B: PDB Molecule:flagellar secretion chaperone flis,flagellin; PDBTitle: crystal structure of the cytoplasmic domain of flha and flis-flic2 complex Phyre2 PDB 1y7m chain A domain 1 Region: 66 - 92 Aligned: 27 Modelled: 27 Confidence: 7.3% Identity: 11% Fold: L,D-transpeptidase catalytic domain-like Superfamily: L,D-transpeptidase catalytic domain-like Family: L,D-transpeptidase catalytic domain-like Phyre2 1 c4kk7A_ 2 c5cyuA_ 3 c6navI_ 4 d2fb5a1 5 d2i5nh1 6 c1k6nH_ 7 c2i5nH_ 8 c5xefA_ 9 d1rzhh1 10 c2k9yB_ 11 c2k9yA_ 12 c1vh6A_ 13 d1vh6a_ 14 c3k1iA_ 15 c2ciuA_ 16 c6f0kA_ 17 c6cfwE_ 18 c2wmmA_ 19 c6ch3B_ 20 d1y7ma1 21 22 23 24 25 26