1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	I	D	R	L	A	K	I	Q	L	S	I	F	A	V	I	T	V	I	T	L	S	V	M	A	I	F	Y	L	R	L	P	A	T	F	G	I	G	T	Y	G	V	S	A	D	F	V	A	G	G	G	L	Y	K	N	A	N	V	T	Y
Secondary structure
SS confidence
Disorder		?	?	?	?																										?	?	?	?	?	?	?	?													?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	G	V	A	V	G	R	V	E	S	V	G	L	N	P	N	G	V	T	A	H	M	R	L	N	S	G	T	A	I	P	S	N	V	T	A	T	V	R	S	V	S	A	I	G	E	Q	Y	I	D	L	V	P	P	E	N	P	S	S	T
Secondary structure
SS confidence
Disorder															?																										?	?	?	?	?	?								?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		K	L	R	N	G	F	R	I	Q	R	Q	N	T	R	I	G	Q	D	V	A	D	L	L	R	Q	A	E	T	L	L	G	S	L	G	D	T	R	L	R	E	L	L	H	E	A	F	I	A	T	N	G	A	G	P	E	L	A	R	L	I
Secondary structure
SS confidence
Disorder		?	?		?				?	?		?	?	?	?	?	?																?	?																?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		E	S	A	R	L	L	V	D	E	A	N	A	N	Y	P	Q	V	S	Q	L	I	D	Q	A	G	P	F	L	Q	A	Q	I	R	A	G	G	D	I	K	S	L	A	D	G	L	A	R	F	T	W	Q	L	R	A	A	D	P	R	L	R
Secondary structure
SS confidence
Disorder													?	?	?																				?																					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		D	T	L	A	D	A	P	D	A	I	D	E	A	N	T	A	F	S	G	I	R	P	S	F	P	A	L	A	A	S	L	A	N	L	G	R	V	G	V	I	Y	H	K	S	I	E	Q	L	L	V	V	F	P	A	L	F	A	A	I	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		T	S	A	G	G	V	P	Q	D	E	G	A	K	L	D	F	K	I	D	L	H	D	P	P	P	C	M	T	G	F	L	P	P	P	L	V	R	S	P	A	D	E	S	V	R	E	I	P	R	D	M	Y	C	K	T	A	Q	N	D	P
Secondary structure
SS confidence
Disorder				?	?	?	?																?	?				?					?	?			?		?	?					?	?	?	?			?							?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		S	T	V	R	G	A	R	N	Y	P	C	Q	E	F	P	G	K	R	A	P	T	V	Q	L	C	R	D	P	R	G	Y	V	P	V	G	T	N	P	W	R	G	P	P	I	P	Y	G	T	E	V	T	D	G	R	N	I	L	P	P	N
Secondary structure
SS confidence
Disorder		?	?	?	?									?	?	?	?	?						?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?	?		?	?	?	?	?	?	?	?	?	?	?		?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		K	F	P	Y	I	P	P	G	A	D	P	D	P	G	V	P	I	V	G	P	P	P	P	G	Q	V	A	G	P	G	P	A	P	H	Q	P	A	Q	P	A	P	P	P	N	D	N	G	P	P	P	P	F	T	S	W	M	P	P	G	Y
Secondary structure
SS confidence
Disorder		?	?	?	?	?			?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		P	P	E	P	P	Q	V	P	Y	P	A	T	I	P	P	P	P	P	P	E	G	T	G	P	P	P	G	P	A	P	G	P	Q	P	Q	A	S	G	P	A	Y	T	I	Y	D	Q	L	S	G	A	F	A	D	P	A	G	G	T	G	I
Secondary structure
SS confidence
Disorder		?	?	?		?	?	?	?	?		?	?	?				?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?							?			?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.
Sequence		F	A	P	G	M	T	G	A	S	S	A	E	N	W	V	D	L	M	R	D	P	R	Q	L
Secondary structure
SS confidence
Disorder				?	?	?	?	?	?	?	?	?								?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 40%) Alpha helix ( 35%) Beta strand ( 11%) TM helix ( 4%) (View membrane topology prediction)

Rank Aligned region

PDB 6ic4 chain C Region: 10 - 144 Aligned: 131 Modelled: 135 Confidence: 99.9% Identity: 35% PDB header:protein transport Chain: C: PDB Molecule:toluene tolerance efflux transporter (abc superfamily, PDBTitle: cryo-em structure of the a. baumannii mla complex at 8.7 a resolution Phyre2 PDB 5uw8 chain C Region: 37 - 134 Aligned: 97 Modelled: 98 Confidence: 99.8% Identity: 26% PDB header:transport protein Chain: C: PDB Molecule:probable phospholipid abc transporter-binding protein mlad; PDBTitle: structure of e. coli mce protein mlad, core mce domain Phyre2 PDB 5uvn chain A Region: 36 - 188 Aligned: 139 Modelled: 121 Confidence: 99.3% Identity: 13% PDB header:transport protein Chain: A: PDB Molecule:paraquat-inducible protein b; PDBTitle: structure of e. coli mce protein pqib, periplasmic domain Phyre2 PDB 5uvn chain E Region: 36 - 188 Aligned: 139 Modelled: 121 Confidence: 99.3% Identity: 13% PDB header:transport protein Chain: E: PDB Molecule:paraquat-inducible protein b; PDBTitle: structure of e. coli mce protein pqib, periplasmic domain Phyre2 PDB 5uvn chain C Region: 36 - 188 Aligned: 139 Modelled: 121 Confidence: 99.3% Identity: 13% PDB header:transport protein Chain: C: PDB Molecule:paraquat-inducible protein b; PDBTitle: structure of e. coli mce protein pqib, periplasmic domain Phyre2 PDB 5uvn chain B Region: 36 - 188 Aligned: 139 Modelled: 121 Confidence: 99.3% Identity: 13% PDB header:transport protein Chain: B: PDB Molecule:paraquat-inducible protein b; PDBTitle: structure of e. coli mce protein pqib, periplasmic domain Phyre2 PDB 5uvn chain D Region: 36 - 188 Aligned: 139 Modelled: 121 Confidence: 99.3% Identity: 13% PDB header:transport protein Chain: D: PDB Molecule:paraquat-inducible protein b; PDBTitle: structure of e. coli mce protein pqib, periplasmic domain Phyre2 PDB 5uvn chain F Region: 36 - 188 Aligned: 139 Modelled: 121 Confidence: 99.3% Identity: 13% PDB header:transport protein Chain: F: PDB Molecule:paraquat-inducible protein b; PDBTitle: structure of e. coli mce protein pqib, periplasmic domain Phyre2 PDB 1qu7 chain A Region: 138 - 295 Aligned: 156 Modelled: 158 Confidence: 96.4% Identity: 10% PDB header:signaling protein Chain: A: PDB Molecule:methyl-accepting chemotaxis protein i; PDBTitle: four helical-bundle structure of the cytoplasmic domain of a serine2 chemotaxis receptor Phyre2 PDB 3g67 chain A Region: 139 - 302 Aligned: 162 Modelled: 164 Confidence: 95.7% Identity: 10% PDB header:signaling protein Chain: A: PDB Molecule:methyl-accepting chemotaxis protein; PDBTitle: crystal structure of a soluble chemoreceptor from thermotoga2 maritima Phyre2 PDB 6e6a chain B Region: 142 - 297 Aligned: 151 Modelled: 156 Confidence: 95.3% Identity: 13% PDB header:protein binding Chain: B: PDB Molecule:inclusion membrane protein a; PDBTitle: triclinic crystal form of inca g144a point mutant Phyre2 PDB 3cwg chain A Region: 139 - 273 Aligned: 135 Modelled: 135 Confidence: 92.9% Identity: 13% PDB header:transcription Chain: A: PDB Molecule:signal transducer and activator of transcription PDBTitle: unphosphorylated mouse stat3 core fragment Phyre2 PDB 5xbj chain A Region: 139 - 258 Aligned: 120 Modelled: 120 Confidence: 92.7% Identity: 11% PDB header:biosynthetic protein Chain: A: PDB Molecule:flagellar hook-associated protein flgk; PDBTitle: the structure of the flagellar hook junction protein hap1 (flgk) from2 campylobacter jejuni Phyre2 PDB 4ut1 chain A Region: 139 - 258 Aligned: 115 Modelled: 120 Confidence: 91.9% Identity: 17% PDB header:motor protein Chain: A: PDB Molecule:flagellar hook-associated protein; PDBTitle: the structure of the flagellar hook junction protein flgk2 from burkholderia pseudomallei Phyre2 PDB 2yfa chain A Region: 139 - 302 Aligned: 164 Modelled: 164 Confidence: 91.2% Identity: 13% PDB header:receptor Chain: A: PDB Molecule:methyl-accepting chemotaxis transducer; PDBTitle: x-ray structure of mcps ligand binding domain in complex with malate Phyre2 PDB 3lnr chain A Region: 162 - 271 Aligned: 110 Modelled: 110 Confidence: 90.1% Identity: 9% PDB header:signaling protein Chain: A: PDB Molecule:aerotaxis transducer aer2; PDBTitle: crystal structure of poly-hamp domains from the p. aeruginosa soluble2 receptor aer2 Phyre2 PDB 2ch7 chain A Region: 139 - 302 Aligned: 164 Modelled: 164 Confidence: 90.0% Identity: 13% PDB header:chemotaxis Chain: A: PDB Molecule:methyl-accepting chemotaxis protein; PDBTitle: crystal structure of the cytoplasmic domain of a bacterial2 chemoreceptor from thermotoga maritima Phyre2 PDB 3zx6 chain A Region: 167 - 287 Aligned: 121 Modelled: 121 Confidence: 87.8% Identity: 11% PDB header:signaling Chain: A: PDB Molecule:hamp, methyl-accepting chemotaxis protein i; PDBTitle: structure of hamp(af1503)-tsr fusion - hamp (a291v) mutant Phyre2 PDB 5u0p chain U Region: 156 - 261 Aligned: 106 Modelled: 106 Confidence: 83.3% Identity: 9% PDB header:transcription Chain: U: PDB Molecule:mediator complex subunit 21; PDBTitle: cryo-em structure of the transcriptional mediator Phyre2 PDB 1quu chain A domain 1 Region: 171 - 292 Aligned: 121 Modelled: 122 Confidence: 82.4% Identity: 13% Fold: Spectrin repeat-like Superfamily: Spectrin repeat Family: Spectrin repeat Phyre2 1 c6ic4C_ 2 c5uw8C_ 3 c5uvnA_ 4 c5uvnE_ 5 c5uvnC_ 6 c5uvnB_ 7 c5uvnD_ 8 c5uvnF_ 9 c1qu7A_ 10 c3g67A_ 11 c6e6aB_ 12 c3cwgA_ 13 c5xbjA_ 14 c4ut1A_ 15 c2yfaA_ 16 c3lnrA_ 17 c2ch7A_ 18 c3zx6A_ 19 c5u0pU_ 20 d1quua1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181