1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	I	F	K	V	G	D	T	V	V	Y	P	H	H	G	A	A	L	V	E	A	I	E	T	R	T	I	K	G	E	Q	K	E	Y	L	V	L	K	V	A	Q	G	D	L	T	V	R	V	P	A	E	N	A	E	Y	V	G	V	R	D
Secondary structure
SS confidence
Disorder		?	?	?	?	?																																																?		?	?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		V	V	G	Q	E	G	L	D	K	V	F	Q	V	L	R	A	P	H	T	E	E	P	T	N	W	S	R	R	Y	K	A	N	L	E	K	L	A	S	G	D	V	N	K	V	A	E	V	V	R	D	L	W	R	R	D	Q	E	R	G	L
Secondary structure
SS confidence
Disorder																			?	?	?	?	?	?	?																															?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.
Sequence		S	A	G	E	K	R	M	L	A	K	A	R	Q	I	L	V	G	E	L	A	L	A	E	S	T	D	D	A	K	A	E	T	I	L	D	E	V	L	A	A	A	S
Secondary structure
SS confidence
Disorder		?																								?													?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 18%) Alpha helix ( 48%) Beta strand ( 20%)

Rank Aligned region

PDB 2lwj chain A Region: 1 - 161 Aligned: 161 Modelled: 161 Confidence: 100.0% Identity: 32% PDB header:transcription Chain: A: PDB Molecule:transcriptional regulator, card family; PDBTitle: nmr solution structure myxoccoccus xanthus cdnl Phyre2 PDB 4l5g chain A Region: 3 - 160 Aligned: 158 Modelled: 158 Confidence: 100.0% Identity: 25% PDB header:transcription Chain: A: PDB Molecule:card; PDBTitle: crystal structure of thermus thermophilus card Phyre2 PDB 4kbm chain B Region: 2 - 160 Aligned: 148 Modelled: 159 Confidence: 100.0% Identity: 99% PDB header:transferase/transcription Chain: B: PDB Molecule:rna polymerase-binding transcription factor card; PDBTitle: structure of the mtb card/rnap beta subunit b1-b2 domains complex Phyre2 PDB 2lt3 chain A Region: 51 - 161 Aligned: 111 Modelled: 111 Confidence: 99.9% Identity: 33% PDB header:transcription Chain: A: PDB Molecule:transcriptional regulator, card family; PDBTitle: solution nmr structure of the c-terminal domain of cdnl from2 myxococcus xanthus Phyre2 PDB 2eyq chain A Region: 4 - 161 Aligned: 140 Modelled: 153 Confidence: 99.9% Identity: 21% PDB header:hydrolase Chain: A: PDB Molecule:transcription-repair coupling factor; PDBTitle: crystal structure of escherichia coli transcription-repair2 coupling factor Phyre2 PDB 2lt4 chain A Region: 2 - 66 Aligned: 65 Modelled: 65 Confidence: 99.8% Identity: 29% PDB header:transcription Chain: A: PDB Molecule:transcriptional regulator, card family; PDBTitle: cdnlnt from myxoccoccus xanthus Phyre2 PDB 2lt1 chain A Region: 1 - 65 Aligned: 65 Modelled: 65 Confidence: 99.8% Identity: 31% PDB header:transcription Chain: A: PDB Molecule:card protein; PDBTitle: solution nmr structure of the 72-residue n-terminal domain of2 myxococcus xanthus card Phyre2 PDB 2lqk chain A Region: 2 - 66 Aligned: 65 Modelled: 65 Confidence: 99.8% Identity: 29% PDB header:transcription regulator Chain: A: PDB Molecule:transcriptional regulator; PDBTitle: nmr solution structure of the n-terminal domain of the cdnl protein2 from thermus thermophilus Phyre2 PDB 2eyq chain A domain 1 Region: 3 - 74 Aligned: 69 Modelled: 72 Confidence: 99.6% Identity: 23% Fold: SH3-like barrel Superfamily: CarD-like Family: CarD-like Phyre2 PDB 3mlq chain E Region: 16 - 53 Aligned: 37 Modelled: 38 Confidence: 98.1% Identity: 27% PDB header:transferase/transcription Chain: E: PDB Molecule:transcription-repair coupling factor; PDBTitle: crystal structure of the thermus thermophilus transcription-repair2 coupling factor rna polymerase interacting domain with the thermus3 aquaticus rna polymerase beta1 domain Phyre2 PDB 3mlq chain H Region: 18 - 60 Aligned: 42 Modelled: 43 Confidence: 98.0% Identity: 24% PDB header:transferase/transcription Chain: H: PDB Molecule:transcription-repair coupling factor; PDBTitle: crystal structure of the thermus thermophilus transcription-repair2 coupling factor rna polymerase interacting domain with the thermus3 aquaticus rna polymerase beta1 domain Phyre2 PDB 5dma chain A Region: 3 - 23 Aligned: 21 Modelled: 21 Confidence: 94.2% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:atp-dependent dna helicase pcra; PDBTitle: crystal structure of c-terminal tudor domain in pcra/uvrd helicase Phyre2 PDB 3t9n chain G Region: 2 - 49 Aligned: 38 Modelled: 48 Confidence: 70.0% Identity: 24% PDB header:membrane protein Chain: G: PDB Molecule:small-conductance mechanosensitive channel; PDBTitle: crystal structure of a membrane protein Phyre2 PDB 2vv5 chain A domain 1 Region: 2 - 49 Aligned: 38 Modelled: 48 Confidence: 52.5% Identity: 24% Fold: Sm-like fold Superfamily: Sm-like ribonucleoproteins Family: Mechanosensitive channel protein MscS (YggB), middle domain Phyre2 PDB 2eyq chain A domain 3 Region: 81 - 160 Aligned: 72 Modelled: 80 Confidence: 52.1% Identity: 17% Fold: P-loop containing nucleoside triphosphate hydrolases Superfamily: P-loop containing nucleoside triphosphate hydrolases Family: Tandem AAA-ATPase domain Phyre2 PDB 1in4 chain A domain 1 Region: 118 - 158 Aligned: 41 Modelled: 41 Confidence: 49.5% Identity: 20% Fold: DNA/RNA-binding 3-helical bundle Superfamily: "Winged helix" DNA-binding domain Family: Helicase DNA-binding domain Phyre2 PDB 2lrq chain A Region: 2 - 53 Aligned: 51 Modelled: 52 Confidence: 49.5% Identity: 22% PDB header:transcription Chain: A: PDB Molecule:nua4 complex subunit eaf3 homolog; PDBTitle: chemical shift assignment and solution structure of fr822a from2 drosophila melanogaster. northeast structural genomics consortium3 target fr822a Phyre2 PDB 6au8 chain A Region: 87 - 135 Aligned: 49 Modelled: 49 Confidence: 48.9% Identity: 16% PDB header:chaperone Chain: A: PDB Molecule:golgi to er traffic protein 4 homolog; PDBTitle: 1.8 angstrom crystal structure of the human bag6-nls & trc35 complex Phyre2 PDB 2vv5 chain D Region: 2 - 49 Aligned: 38 Modelled: 48 Confidence: 44.0% Identity: 24% PDB header:membrane protein Chain: D: PDB Molecule:small-conductance mechanosensitive channel; PDBTitle: the open structure of mscs Phyre2 PDB 4hw9 chain E Region: 3 - 49 Aligned: 37 Modelled: 47 Confidence: 42.7% Identity: 27% PDB header:membrane protein Chain: E: PDB Molecule:mechanosensitive channel mscs; PDBTitle: crystal structure of helicobacter pylori mscs (closed state) Phyre2 1 c2lwjA_ 2 c4l5gA_ 3 c4kbmB_ 4 c2lt3A_ 5 c2eyqA_ 6 c2lt4A_ 7 c2lt1A_ 8 c2lqkA_ 9 d2eyqa1 10 c3mlqE_ 11 c3mlqH_ 12 c5dmaA_ 13 c3t9nG_ 14 d2vv5a1 15 d2eyqa3 16 d1in4a1 17 c2lrqA_ 18 c6au8A_ 19 c2vv5D_ 20 c4hw9E_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99