1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	G	I	A	S	A	A	L	I	L	S	L	A	L	V	V	L	P	G	S	P	R	C	R	L	T	P	D	D	T	G	R	R	V	L	L	V	G	A	R	R	V	A	W	G	V	G	C	V	A	V	G	V	A	A	L	L	P	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?											?	?	?	?	?	?			?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		P	T	V	V	A	V	A	V	L	G	A	T	L	G	L	R	Y	R	R	R	R	R	Y	L	R	R	S	R	E	G	Q	A	L	E	A	A	L	E	L	V	V	G	E	L	R	A	G	A	H	P	V	R	A	F	S	I	A	A	D	E
Secondary structure
SS confidence
Disorder																																													?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		T	G	G	P	V	A	V	A	L	R	A	V	A	A	R	A	R	L	G	A	D	V	T	A	G	L	L	A	A	A	R	S	S	A	L	P	A	Y	W	E	R	L	A	V	C	W	Q	L	G	S	D	H	G	L	A	I	A	S	L	M
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		R	A	A	Q	R	D	V	A	E	R	Q	R	F	S	A	R	V	S	A	G	M	A	G	A	R	A	S	A	A	I	L	A	I	L	P	L	L	G	V	L	L	G	Q	L	I	G	A	R	P	L	S	F	L	L	T	G	R	V	G	G
Secondary structure
SS confidence
Disorder																						?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.
Sequence		W	L	L	V	V	G	L	T	L	A	C	A	G	L	L	W	S	D	R	I	T	D	R	P	V	L
Secondary structure
SS confidence
Disorder																							?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 13%) Alpha helix ( 85%) Beta strand ( 0%) TM helix ( 27%) (View membrane topology prediction)

Rank Aligned region

PDB 3c1q chain A Region: 90 - 201 Aligned: 106 Modelled: 112 Confidence: 99.2% Identity: 14% PDB header:transport protein Chain: A: PDB Molecule:general secretion pathway protein f; PDBTitle: the three-dimensional structure of the cytoplasmic domains of epsf2 from the type 2 secretion system of vibrio cholerae Phyre2 PDB 5nbg chain A Region: 90 - 198 Aligned: 103 Modelled: 109 Confidence: 99.2% Identity: 15% PDB header:membrane protein Chain: A: PDB Molecule:general secretion pathway protein f; PDBTitle: structure of the cytoplasmic domain i of outf in the d. dadantii type2 ii secretion system Phyre2 PDB 2whn chain A Region: 90 - 194 Aligned: 98 Modelled: 105 Confidence: 98.3% Identity: 11% PDB header:protein transport Chain: A: PDB Molecule:pilus assembly protein pilc; PDBTitle: n-terminal domain from the pilc type iv pilus biogenesis protein Phyre2 PDB 4hhx chain A Region: 90 - 187 Aligned: 89 Modelled: 98 Confidence: 97.3% Identity: 13% PDB header:membrane protein Chain: A: PDB Molecule:toxin coregulated pilus biosynthesis protein e; PDBTitle: structure of cytoplasmic domain of tcpe from vibrio cholerae Phyre2 PDB 5n9y chain B Region: 125 - 259 Aligned: 133 Modelled: 135 Confidence: 89.1% Identity: 12% PDB header:membrane protein Chain: B: PDB Molecule:zinc transport protein zntb; PDBTitle: the full-length structure of zntb Phyre2 PDB 6bs9 chain A Region: 82 - 196 Aligned: 113 Modelled: 115 Confidence: 82.2% Identity: 19% PDB header:transport protein Chain: A: PDB Molecule:stage iii sporulation protein ab; PDBTitle: stage iii sporulation protein ab (spoiiiab) Phyre2 PDB 4i0x chain A Region: 89 - 155 Aligned: 67 Modelled: 67 Confidence: 35.0% Identity: 22% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:esat-6-like protein mab_3112; PDBTitle: crystal structure of the mycobacterum abscessus esxef (mab_3112-2 mab_3113) complex Phyre2 PDB 4ev6 chain E Region: 84 - 259 Aligned: 165 Modelled: 171 Confidence: 32.5% Identity: 13% PDB header:metal transport Chain: E: PDB Molecule:magnesium transport protein cora; PDBTitle: the complete structure of cora magnesium transporter from2 methanocaldococcus jannaschii Phyre2 PDB 5eze chain G Region: 174 - 201 Aligned: 28 Modelled: 28 Confidence: 27.9% Identity: 18% PDB header:de novo protein Chain: G: PDB Molecule:cc-hept-bmecys-his-glu; PDBTitle: a de novo designed heptameric coiled coil cc-hept-i18betamecys-l22h-2 i25e Phyre2 PDB 5eze chain F Region: 174 - 199 Aligned: 26 Modelled: 26 Confidence: 23.0% Identity: 19% PDB header:de novo protein Chain: F: PDB Molecule:cc-hept-bmecys-his-glu; PDBTitle: a de novo designed heptameric coiled coil cc-hept-i18betamecys-l22h-2 i25e Phyre2 PDB 5f2y chain A Region: 174 - 199 Aligned: 26 Modelled: 26 Confidence: 23.0% Identity: 19% PDB header:de novo protein Chain: A: PDB Molecule:cc-hept-hcys-h-e; PDBTitle: a de novo designed heptameric coiled coil cc-hept-homocys-h-e Phyre2 PDB 5eze chain A Region: 174 - 199 Aligned: 26 Modelled: 26 Confidence: 23.0% Identity: 19% PDB header:de novo protein Chain: A: PDB Molecule:cc-hept-bmecys-his-glu; PDBTitle: a de novo designed heptameric coiled coil cc-hept-i18betamecys-l22h-2 i25e Phyre2 PDB 5eze chain E Region: 174 - 199 Aligned: 26 Modelled: 26 Confidence: 22.9% Identity: 19% PDB header:de novo protein Chain: E: PDB Molecule:cc-hept-bmecys-his-glu; PDBTitle: a de novo designed heptameric coiled coil cc-hept-i18betamecys-l22h-2 i25e Phyre2 PDB 5f2y chain E Region: 174 - 199 Aligned: 26 Modelled: 26 Confidence: 22.9% Identity: 19% PDB header:de novo protein Chain: E: PDB Molecule:cc-hept-hcys-h-e; PDBTitle: a de novo designed heptameric coiled coil cc-hept-homocys-h-e Phyre2 PDB 5eze chain C Region: 174 - 199 Aligned: 26 Modelled: 26 Confidence: 22.9% Identity: 19% PDB header:de novo protein Chain: C: PDB Molecule:cc-hept-bmecys-his-glu; PDBTitle: a de novo designed heptameric coiled coil cc-hept-i18betamecys-l22h-2 i25e Phyre2 PDB 6a6x chain C Region: 169 - 209 Aligned: 41 Modelled: 41 Confidence: 22.7% Identity: 17% PDB header:toxin Chain: C: PDB Molecule:antitoxin maze7; PDBTitle: the crystal structure of the mtb maze-mazf-mt9 complex Phyre2 PDB 5f2y chain G Region: 174 - 199 Aligned: 26 Modelled: 26 Confidence: 22.5% Identity: 19% PDB header:de novo protein Chain: G: PDB Molecule:cc-hept-hcys-h-e; PDBTitle: a de novo designed heptameric coiled coil cc-hept-homocys-h-e Phyre2 PDB 5f2y chain F Region: 174 - 196 Aligned: 23 Modelled: 23 Confidence: 10.9% Identity: 17% PDB header:de novo protein Chain: F: PDB Molecule:cc-hept-hcys-h-e; PDBTitle: a de novo designed heptameric coiled coil cc-hept-homocys-h-e Phyre2 PDB 5f2y chain B Region: 174 - 196 Aligned: 23 Modelled: 23 Confidence: 10.0% Identity: 17% PDB header:de novo protein Chain: B: PDB Molecule:cc-hept-hcys-h-e; PDBTitle: a de novo designed heptameric coiled coil cc-hept-homocys-h-e Phyre2 PDB 3l8j chain A Region: 93 - 205 Aligned: 108 Modelled: 113 Confidence: 8.1% Identity: 17% PDB header:protein binding Chain: A: PDB Molecule:programmed cell death protein 10; PDBTitle: crystal structure of ccm3, a cerebral cavernous malformation protein2 critical for vascular integrity Phyre2 1 c3c1qA_ 2 c5nbgA_ 3 c2whnA_ 4 c4hhxA_ 5 c5n9yB_ 6 c6bs9A_ 7 c4i0xA_ 8 c4ev6E_ 9 c5ezeG_ 10 c5ezeF_ 11 c5f2yA_ 12 c5ezeA_ 13 c5ezeE_ 14 c5f2yE_ 15 c5ezeC_ 16 c6a6xC_ 17 c5f2yG_ 18 c5f2yF_ 19 c5f2yB_ 20 c3l8jA_ 21