1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	R	T	I	S	P	F	L	R	C	R	H	E	T	C	C	I	S	N	V	G	E	E	V	T	R	T	T	Y	S	R	E	H	Q	R	E	Y	R	R	K	V	R	L	C	L	D	V	F	E	T	M	L	A	Q	T	R	F	E	A	D
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?									?	?										?	?	?	?																														?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	P	L	T	G	M	E	I	E	C	N	L	V	D	A	D	Y	Q	P	A	M	S	N	R	Y	V	L	D	A	I	A	D	P	A	Y	Q	T	E	L	G	A	Y	N	I	E	F	N	V	P	P	R	P	L	P	G	R	T	C	L	E
Secondary structure
SS confidence
Disorder																																																						?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	E	D	E	V	R	A	S	L	N	D	A	E	T	K	A	S	C	S	G	A	H	I	V	M	I	G	I	L	P	T	L	M	P	E	H	L	T	D	G	W	M	S	A	S	A	R	Y	A	A	L	N	E	S	I	F	K	A	R	G
Secondary structure
SS confidence
Disorder																																						?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		E	D	I	P	I	N	I	A	G	P	E	P	L	S	C	H	A	G	S	I	A	P	E	S	A	C	T	S	V	Q	L	H	L	Q	L	A	P	A	D	F	P	A	N	W	N	A	A	Q	V	L	A	G	P	Q	L	A	L	G	A	N
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		S	P	Y	F	F	G	H	Q	L	W	S	E	T	R	I	E	L	F	T	Q	S	T	D	A	R	P	E	E	L	K	S	R	G	V	R	P	R	V	W	F	G	E	R	W	I	T	S	V	L	D	L	F	Q	E	N	I	R	Y	F	P
Secondary structure
SS confidence
Disorder																														?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		T	L	L	P	E	V	S	D	E	D	P	L	A	E	L	S	A	G	R	I	P	H	L	S	E	L	R	L	H	N	G	T	V	Y	R	W	N	R	P	V	Y	D	V	V	D	G	R	P	H	L	R	L	E	N	R	V	L	P	A	G
Secondary structure
SS confidence
Disorder									?		?	?			?																														?															?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		P	T	V	V	D	M	L	A	N	H	A	F	Y	Y	G	A	L	R	G	L	S	E	A	D	P	P	L	W	T	Q	M	N	F	A	A	A	Q	A	N	F	L	A	A	A	R	Y	G	M	D	A	Q	L	D	W	P	G	L	G	E	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		T	T	R	E	L	V	L	G	T	L	L	P	M	A	H	E	G	L	R	R	W	G	V	D	A	E	V	R	D	R	F	L	G	V	I	G	G	R	A	Q	T	G	R	N	G	A	R	W	Q	V	A	T	V	A	A	L	Q	D	G	G
Secondary structure
SS confidence
Disorder																																										?	?	?	?												?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.
Sequence		L	T	R	P	A	A	L	A	E	M	L	R	R	Y	C	E	H	M	H	S	N	E	P	V	H	T	W	D	T
Secondary structure
SS confidence
Disorder		?	?	?																	?			?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 9%) Alpha helix ( 55%) Beta strand ( 6%)

Rank Aligned region

PDB 3lvw chain A Region: 21 - 502 Aligned: 481 Modelled: 482 Confidence: 100.0% Identity: 17% PDB header:ligase Chain: A: PDB Molecule:glutamate--cysteine ligase; PDBTitle: glutathione-inhibited scgcl Phyre2 PDB 1r8g chain A Region: 55 - 499 Aligned: 344 Modelled: 362 Confidence: 100.0% Identity: 19% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamate-cysteine ligase family 2 (GCS2) Phyre2 PDB 1tt4 chain A Region: 56 - 497 Aligned: 345 Modelled: 364 Confidence: 100.0% Identity: 18% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamate-cysteine ligase family 2 (GCS2) Phyre2 PDB 1tt4 chain B Region: 56 - 497 Aligned: 345 Modelled: 372 Confidence: 100.0% Identity: 18% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:putative cytoplasmic protein; PDBTitle: structure of np459575, a predicted glutathione synthase from2 salmonella typhimurium Phyre2 PDB 2gwc chain E Region: 46 - 490 Aligned: 399 Modelled: 420 Confidence: 100.0% Identity: 20% PDB header:ligase Chain: E: PDB Molecule:glutamate cysteine ligase; PDBTitle: crystal structure of plant glutamate cysteine ligase in complex with a2 transition state analogue Phyre2 PDB 2d32 chain A domain 1 Region: 46 - 477 Aligned: 400 Modelled: 413 Confidence: 99.9% Identity: 16% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamate-cysteine ligase Phyre2 PDB 3nzt chain A Region: 56 - 477 Aligned: 386 Modelled: 394 Confidence: 99.8% Identity: 13% PDB header:ligase Chain: A: PDB Molecule:glutamate--cysteine ligase; PDBTitle: 2.0 angstrom crystal structure of glutamate--cysteine ligase (gsha)2 ftom francisella tularensis in complex with amp Phyre2 PDB 3ln6 chain A Region: 63 - 502 Aligned: 360 Modelled: 395 Confidence: 99.6% Identity: 13% PDB header:ligase Chain: A: PDB Molecule:glutathione biosynthesis bifunctional protein gshab; PDBTitle: crystal structure of a bifunctional glutathione synthetase from2 streptococcus agalactiae Phyre2 PDB 3ln7 chain A Region: 63 - 505 Aligned: 377 Modelled: 427 Confidence: 99.5% Identity: 13% PDB header:ligase Chain: A: PDB Molecule:glutathione biosynthesis bifunctional protein gshab; PDBTitle: crystal structure of a bifunctional glutathione synthetase from2 pasteurella multocida Phyre2 PDB 2bvc chain A domain 2 Region: 38 - 389 Aligned: 219 Modelled: 244 Confidence: 98.9% Identity: 22% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamine synthetase catalytic domain Phyre2 PDB 1hto chain B Region: 38 - 389 Aligned: 213 Modelled: 224 Confidence: 98.8% Identity: 24% PDB header:ligase Chain: B: PDB Molecule:glutamine synthetase; PDBTitle: crystallographic structure of a relaxed glutamine synthetase from2 mycobacterium tuberculosis Phyre2 PDB 1f52 chain A domain 2 Region: 38 - 389 Aligned: 218 Modelled: 228 Confidence: 98.8% Identity: 22% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamine synthetase catalytic domain Phyre2 PDB 3ng0 chain A Region: 38 - 389 Aligned: 215 Modelled: 225 Confidence: 98.8% Identity: 21% PDB header:ligase Chain: A: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from synechocystis sp. pcc2 6803 Phyre2 PDB 4hpp chain A Region: 38 - 476 Aligned: 281 Modelled: 288 Confidence: 98.7% Identity: 19% PDB header:ligase Chain: A: PDB Molecule:probable glutamine synthetase; PDBTitle: crystal structure of novel glutamine synthase homolog Phyre2 PDB 5zlp chain H Region: 38 - 389 Aligned: 215 Modelled: 230 Confidence: 98.7% Identity: 25% PDB header:ligase Chain: H: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from helicobacter pylori Phyre2 PDB 4s17 chain E Region: 38 - 389 Aligned: 213 Modelled: 228 Confidence: 98.7% Identity: 23% PDB header:ligase Chain: E: PDB Molecule:glutamine synthetase; PDBTitle: the crystal structure of glutamine synthetase from bifidobacterium2 adolescentis atcc 15703 Phyre2 PDB 5dm3 chain A Region: 46 - 389 Aligned: 205 Modelled: 215 Confidence: 98.6% Identity: 21% PDB header:ligase Chain: A: PDB Molecule:l-glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from chromohalobacter2 salexigens dsm 3043(csal_0679, target efi-550015) with bound adp Phyre2 PDB 1fpy chain E Region: 42 - 389 Aligned: 216 Modelled: 218 Confidence: 98.6% Identity: 26% PDB header:ligase Chain: E: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from salmonella2 typhimurium with inhibitor phosphinothricin Phyre2 PDB 3qaj chain L Region: 46 - 387 Aligned: 204 Modelled: 218 Confidence: 98.5% Identity: 18% PDB header:ligase Chain: L: PDB Molecule:glutamine synthetase; PDBTitle: x-ray crystal structure of glutamine synthetase from bacillus subtilis2 cocrystallized with atp Phyre2 PDB 3o6x chain C Region: 36 - 405 Aligned: 248 Modelled: 264 Confidence: 98.2% Identity: 15% PDB header:ligase Chain: C: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the type iii glutamine synthetase from2 bacteroides fragilis Phyre2 1 c3lvwA_ 2 d1r8ga_ 3 d1tt4a_ 4 c1tt4B_ 5 c2gwcE_ 6 d2d32a1 7 c3nztA_ 8 c3ln6A_ 9 c3ln7A_ 10 d2bvca2 11 c1htoB_ 12 d1f52a2 13 c3ng0A_ 14 c4hppA_ 15 c5zlpH_ 16 c4s17E_ 17 c5dm3A_ 18 c1fpyE_ 19 c3qajL_ 20 c3o6xC_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86