1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	C	R	H	L	G	W	L	G	A	Q	V	A	V	S	S	L	V	L	D	P	P	Q	G	L	R	V	Q	S	Y	A	P	R	R	Q	K	H	G	L	M	N	A	D	G	W	G	V	G	F	F	D	G	A	I	P	R	R	W	R	S
Secondary structure
SS confidence
Disorder		?	?	?																												?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		P	A	P	L	W	G	D	T	S	F	H	S	V	A	P	A	L	R	S	H	C	I	L	A	A	V	R	S	A	T	V	G	M	P	I	E	V	S	A	T	P	P	F	T	D	G	H	W	L	L	A	H	N	G	V	V	D	R	A	V
Secondary structure
SS confidence
Disorder					?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	P	A	G	P	A	A	E	S	V	C	D	S	A	I	L	A	A	T	I	F	A	H	G	L	D	A	L	G	D	T	I	V	K	V	G	A	A	D	P	N	A	R	L	N	I	L	A	A	N	G	S	R	L	I	A	T	T	W	G
Secondary structure
SS confidence
Disorder			?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.
Sequence		D	T	L	S	I	L	R	R	A	D	G	V	V	L	A	S	E	P	Y	D	D	D	S	G	W	G	D	V	P	D	R	H	L	V	E	V	T	Q	K	G	V	T	L	T	A	L	D	R	A	K	G	P	R
Secondary structure
SS confidence
Disorder																							?																									?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 13%) Alpha helix ( 24%) Beta strand ( 33%)

Rank Aligned region

PDB 4zfj chain B Region: 2 - 226 Aligned: 225 Modelled: 225 Confidence: 100.0% Identity: 73% PDB header:hydrolase Chain: B: PDB Molecule:amidohydrolase egtc; PDBTitle: ergothioneine-biosynthetic ntn hydrolase egtc, apo form Phyre2 PDB 3mdn chain D Region: 2 - 230 Aligned: 192 Modelled: 203 Confidence: 100.0% Identity: 25% PDB header:transferase Chain: D: PDB Molecule:glutamine aminotransferase class-ii domain protein; PDBTitle: structure of glutamine aminotransferase class-ii domain protein2 (spo2029) from silicibacter pomeroyi Phyre2 PDB 1te5 chain A Region: 2 - 224 Aligned: 210 Modelled: 211 Confidence: 100.0% Identity: 24% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1ecf chain A domain 2 Region: 2 - 233 Aligned: 217 Modelled: 232 Confidence: 100.0% Identity: 24% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1ecj chain B Region: 2 - 233 Aligned: 218 Modelled: 232 Confidence: 100.0% Identity: 22% PDB header:transferase Chain: B: PDB Molecule:glutamine phosphoribosylpyrophosphate PDBTitle: escherichia coli glutamine phosphoribosylpyrophosphate2 (prpp) amidotransferase complexed with 2 amp per tetramer Phyre2 PDB 1gph chain 1 Region: 2 - 233 Aligned: 216 Modelled: 232 Confidence: 100.0% Identity: 21% PDB header:transferase Chain: 1: PDB Molecule:glutamine phosphoribosyl-pyrophosphate amidotransferase; PDBTitle: structure of the allosteric regulatory enzyme of purine biosynthesis Phyre2 PDB 1gph chain 1 domain 2 Region: 2 - 228 Aligned: 212 Modelled: 227 Confidence: 100.0% Identity: 21% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1xff chain A Region: 2 - 233 Aligned: 217 Modelled: 220 Confidence: 100.0% Identity: 18% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1jxa chain A Region: 2 - 233 Aligned: 217 Modelled: 220 Confidence: 100.0% Identity: 18% PDB header:transferase Chain: A: PDB Molecule:glucosamine 6-phosphate synthase; PDBTitle: glucosamine 6-phosphate synthase with glucose 6-phosphate Phyre2 PDB 1ct9 chain A domain 2 Region: 2 - 223 Aligned: 173 Modelled: 194 Confidence: 99.9% Identity: 17% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1ct9 chain D Region: 2 - 223 Aligned: 170 Modelled: 195 Confidence: 99.9% Identity: 17% PDB header:ligase Chain: D: PDB Molecule:asparagine synthetase b; PDBTitle: crystal structure of asparagine synthetase b from2 escherichia coli Phyre2 PDB 1ofd chain A domain 3 Region: 54 - 223 Aligned: 164 Modelled: 170 Confidence: 99.8% Identity: 20% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1ea0 chain A domain 3 Region: 56 - 223 Aligned: 162 Modelled: 163 Confidence: 99.8% Identity: 17% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1jgt chain A domain 2 Region: 39 - 227 Aligned: 173 Modelled: 189 Confidence: 99.7% Identity: 15% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1m1z chain B Region: 74 - 232 Aligned: 146 Modelled: 159 Confidence: 99.7% Identity: 16% PDB header:hydrolase Chain: B: PDB Molecule:beta-lactam synthetase; PDBTitle: beta-lactam synthetase apo enzyme Phyre2 PDB 1q15 chain A Region: 51 - 233 Aligned: 166 Modelled: 183 Confidence: 99.4% Identity: 14% PDB header:biosynthetic protein Chain: A: PDB Molecule:cara; PDBTitle: carbapenam synthetase Phyre2 PDB 1q15 chain A domain 2 Region: 96 - 220 Aligned: 116 Modelled: 125 Confidence: 99.3% Identity: 17% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 2vdc chain F Region: 54 - 217 Aligned: 158 Modelled: 164 Confidence: 99.1% Identity: 18% PDB header:oxidoreductase Chain: F: PDB Molecule:glutamate synthase [nadph] large chain; PDBTitle: the 9.5 a resolution structure of glutamate synthase from cryo-2 electron microscopy and its oligomerization behavior in solution:3 functional implications. Phyre2 PDB 1lm1 chain A Region: 54 - 217 Aligned: 158 Modelled: 164 Confidence: 99.1% Identity: 18% PDB header:oxidoreductase Chain: A: PDB Molecule:ferredoxin-dependent glutamate synthase; PDBTitle: structural studies on the synchronization of catalytic centers in2 glutamate synthase: native enzyme Phyre2 PDB 5vrv chain A Region: 145 - 208 Aligned: 64 Modelled: 64 Confidence: 26.9% Identity: 9% PDB header:hydrolase,oxidoreductase Chain: A: PDB Molecule:protein regulated by acid ph; PDBTitle: 2.05 angstrom resolution crystal structure of c-terminal domain2 (duf2156) of putative lysylphosphatidylglycerol synthetase from3 agrobacterium fabrum. Phyre2 1 c4zfjB_ 2 c3mdnD_ 3 d1te5a_ 4 d1ecfa2 5 c1ecjB_ 6 c1gph1_ 7 d1gph12 8 d1xffa_ 9 c1jxaA_ 10 d1ct9a2 11 c1ct9D_ 12 d1ofda3 13 d1ea0a3 14 d1jgta2 15 c1m1zB_ 16 c1q15A_ 17 d1q15a2 18 c2vdcF_ 19 c1lm1A_ 20 c5vrvA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67