1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	L	A	A	M	T	A	A	A	S	Q	L	D	N	A	A	P	D	D	V	E	I	T	D	S	S	A	A	A	E	Y	I	A	D	G	C	L	V	D	G	P	L	G	R	V	G	L	E	M	E	A	H	C	F	D	P	A	D	P
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?				?	?																?	?	?																		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		F	R	R	P	S	W	E	E	I	T	E	V	L	E	W	L	S	P	L	P	G	G	S	V	V	S	V	E	P	G	G	A	V	E	L	S	G	P	P	A	D	G	V	L	A	A	I	G	A	M	T	R	D	Q	A	V	L	R	S	A
Secondary structure
SS confidence
Disorder			?	?	?	?										?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	A	N	A	G	L	G	L	V	F	L	G	A	D	P	L	R	S	P	V	R	V	N	P	G	A	R	Y	R	A	M	E	Q	F	F	A	A	S	H	S	G	V	P	G	A	A	M	M	T	S	T	A	A	I	Q	V	N	L	D	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		G	P	Q	E	G	W	A	E	R	V	R	L	A	H	A	L	G	P	T	M	I	A	I	A	A	N	S	P	M	L	G	G	R	F	S	G	W	Q	S	T	R	Q	R	V	W	G	Q	M	D	S	A	R	C	G	P	I	L	G	A	S
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		G	D	H	P	G	I	D	W	A	K	Y	A	L	K	A	P	V	M	M	V	R	S	P	D	T	Q	D	T	R	A	V	T	D	Y	V	P	F	T	D	W	V	D	G	R	V	L	L	D	G	R	R	A	T	V	A	D	L	V	Y	H
Secondary structure
SS confidence
Disorder																																																?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	T	T	L	F	P	P	V	R	P	R	Q	W	L	E	I	R	Y	L	D	S	V	P	D	E	V	W	P	A	V	V	F	T	L	V	T	L	L	D	D	P	V	A	A	D	L	A	V	D	A	V	E	P	V	A	T	A	W	D	T
Secondary structure
SS confidence
Disorder																																													?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		A	A	R	I	G	L	A	D	R	R	L	Y	L	A	A	N	R	C	L	A	I	A	A	R	R	V	P	T	E	L	I	G	A	M	Q	R	L	V	D	H	V	D	R	G	V	C	P	A	D	D	F	S	D	R	V	I	A	G	G	I
Secondary structure
SS confidence
Disorder																																															?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.
Sequence		A	S	A	V	T	G	M	M	H	G	A	S
Secondary structure
SS confidence
Disorder										?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 11%) Alpha helix ( 51%) Beta strand ( 6%)

Rank Aligned region

PDB 2gwc chain E Region: 21 - 429 Aligned: 401 Modelled: 409 Confidence: 100.0% Identity: 24% PDB header:ligase Chain: E: PDB Molecule:glutamate cysteine ligase; PDBTitle: crystal structure of plant glutamate cysteine ligase in complex with a2 transition state analogue Phyre2 PDB 1tt4 chain A Region: 38 - 429 Aligned: 330 Modelled: 352 Confidence: 100.0% Identity: 20% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamate-cysteine ligase family 2 (GCS2) Phyre2 PDB 1tt4 chain B Region: 38 - 429 Aligned: 330 Modelled: 352 Confidence: 100.0% Identity: 20% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:putative cytoplasmic protein; PDBTitle: structure of np459575, a predicted glutathione synthase from2 salmonella typhimurium Phyre2 PDB 1r8g chain A Region: 38 - 429 Aligned: 326 Modelled: 338 Confidence: 100.0% Identity: 20% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamate-cysteine ligase family 2 (GCS2) Phyre2 PDB 2d32 chain A domain 1 Region: 47 - 429 Aligned: 350 Modelled: 359 Confidence: 99.7% Identity: 17% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamate-cysteine ligase Phyre2 PDB 3nzt chain A Region: 47 - 429 Aligned: 347 Modelled: 359 Confidence: 99.5% Identity: 15% PDB header:ligase Chain: A: PDB Molecule:glutamate--cysteine ligase; PDBTitle: 2.0 angstrom crystal structure of glutamate--cysteine ligase (gsha)2 ftom francisella tularensis in complex with amp Phyre2 PDB 3lvw chain A Region: 39 - 339 Aligned: 277 Modelled: 280 Confidence: 99.5% Identity: 22% PDB header:ligase Chain: A: PDB Molecule:glutamate--cysteine ligase; PDBTitle: glutathione-inhibited scgcl Phyre2 PDB 3ln6 chain A Region: 44 - 429 Aligned: 331 Modelled: 344 Confidence: 99.4% Identity: 16% PDB header:ligase Chain: A: PDB Molecule:glutathione biosynthesis bifunctional protein gshab; PDBTitle: crystal structure of a bifunctional glutathione synthetase from2 streptococcus agalactiae Phyre2 PDB 3ln7 chain A Region: 44 - 424 Aligned: 328 Modelled: 340 Confidence: 99.3% Identity: 13% PDB header:ligase Chain: A: PDB Molecule:glutathione biosynthesis bifunctional protein gshab; PDBTitle: crystal structure of a bifunctional glutathione synthetase from2 pasteurella multocida Phyre2 PDB 4hpp chain A Region: 45 - 337 Aligned: 200 Modelled: 219 Confidence: 98.4% Identity: 19% PDB header:ligase Chain: A: PDB Molecule:probable glutamine synthetase; PDBTitle: crystal structure of novel glutamine synthase homolog Phyre2 PDB 5zlp chain H Region: 45 - 337 Aligned: 185 Modelled: 209 Confidence: 98.4% Identity: 19% PDB header:ligase Chain: H: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from helicobacter pylori Phyre2 PDB 3qaj chain L Region: 26 - 337 Aligned: 203 Modelled: 218 Confidence: 98.4% Identity: 18% PDB header:ligase Chain: L: PDB Molecule:glutamine synthetase; PDBTitle: x-ray crystal structure of glutamine synthetase from bacillus subtilis2 cocrystallized with atp Phyre2 PDB 2bvc chain A domain 2 Region: 45 - 208 Aligned: 132 Modelled: 145 Confidence: 98.2% Identity: 17% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamine synthetase catalytic domain Phyre2 PDB 1f52 chain A domain 2 Region: 45 - 208 Aligned: 131 Modelled: 133 Confidence: 98.2% Identity: 18% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamine synthetase catalytic domain Phyre2 PDB 1hto chain B Region: 41 - 208 Aligned: 136 Modelled: 149 Confidence: 98.2% Identity: 17% PDB header:ligase Chain: B: PDB Molecule:glutamine synthetase; PDBTitle: crystallographic structure of a relaxed glutamine synthetase from2 mycobacterium tuberculosis Phyre2 PDB 3ng0 chain A Region: 41 - 208 Aligned: 135 Modelled: 149 Confidence: 98.2% Identity: 16% PDB header:ligase Chain: A: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from synechocystis sp. pcc2 6803 Phyre2 PDB 2qc8 chain J Region: 22 - 207 Aligned: 151 Modelled: 168 Confidence: 98.0% Identity: 13% PDB header:ligase Chain: J: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of human glutamine synthetase in complex with adp2 and methionine sulfoximine phosphate Phyre2 PDB 1fpy chain E Region: 45 - 208 Aligned: 130 Modelled: 136 Confidence: 98.0% Identity: 18% PDB header:ligase Chain: E: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from salmonella2 typhimurium with inhibitor phosphinothricin Phyre2 PDB 3o6x chain C Region: 44 - 337 Aligned: 191 Modelled: 204 Confidence: 97.9% Identity: 16% PDB header:ligase Chain: C: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the type iii glutamine synthetase from2 bacteroides fragilis Phyre2 PDB 4s17 chain E Region: 45 - 208 Aligned: 131 Modelled: 144 Confidence: 97.9% Identity: 18% PDB header:ligase Chain: E: PDB Molecule:glutamine synthetase; PDBTitle: the crystal structure of glutamine synthetase from bifidobacterium2 adolescentis atcc 15703 Phyre2 1 c2gwcE_ 2 d1tt4a_ 3 c1tt4B_ 4 d1r8ga_ 5 d2d32a1 6 c3nztA_ 7 c3lvwA_ 8 c3ln6A_ 9 c3ln7A_ 10 c4hppA_ 11 c5zlpH_ 12 c3qajL_ 13 d2bvca2 14 d1f52a2 15 c1htoB_ 16 c3ng0A_ 17 c2qc8J_ 18 c1fpyE_ 19 c3o6xC_ 20 c4s17E_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64