1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	P	P	E	S	R	P	G	P	D	S	P	P	T	D	E	L	A	C	A	E	A	A	L	Q	V	L	Q	Q	V	L	H	T	I	G	R	Q	D	K	A	K	Q	T	P	C	P	G	Y	D	V	K	K	L	T	E	H	L	L	N	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?		?		?																											?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	M	V	L	G	G	M	V	G	A	E	F	S	L	R	A	D	I	D	S	V	E	R	L	V	S	G	A	A	R	S	A	L	D	A	W	H	R	H	G	L	E	G	D	V	S	L	G	P	G	S	M	S	A	K	V	A	V	S	V
Secondary structure
SS confidence
Disorder													?	?	?	?	?																															?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		F	S	V	E	F	L	V	H	A	W	D	Y	A	V	A	V	G	S	E	L	K	A	A	D	S	L	A	E	Y	V	L	E	L	A	R	K	L	I	K	P	E	E	R	S	V	A	G	F	N	E	P	V	D	V	P	E	D	G	G	A
Secondary structure
SS confidence
Disorder																																								?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.
Sequence		L	E	R	L	I	A	F	T	G	R	N	P	A	R
Secondary structure
SS confidence
Disorder											?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 16%) Alpha helix ( 57%) Beta strand ( 0%)

Rank Aligned region

PDB 2nsf chain A Region: 10 - 194 Aligned: 185 Modelled: 185 Confidence: 100.0% Identity: 15% PDB header:isomerase Chain: A: PDB Molecule:hypothetical protein cgl3021; PDBTitle: crystal structure of the mycothiol-dependent maleylpyruvate isomerase Phyre2 PDB 2nsf chain A domain 1 Region: 10 - 139 Aligned: 130 Modelled: 128 Confidence: 100.0% Identity: 16% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: Maleylpyruvate isomerase-like Phyre2 PDB 6iz2 chain A Region: 11 - 141 Aligned: 131 Modelled: 131 Confidence: 98.4% Identity: 10% PDB header:unknown function Chain: A: PDB Molecule:dinb/yfit family protein; PDBTitle: crystal structure of dinb/yfit protein dr0053 from d. radiodurans r1 Phyre2 PDB 5cog chain B Region: 15 - 68 Aligned: 54 Modelled: 54 Confidence: 98.2% Identity: 11% PDB header:unknown function Chain: B: PDB Molecule:irc4; PDBTitle: crystal structure of yeast irc4 Phyre2 PDB 2rd9 chain C Region: 16 - 141 Aligned: 126 Modelled: 126 Confidence: 98.1% Identity: 13% PDB header:hydrolase Chain: C: PDB Molecule:bh0186 protein; PDBTitle: crystal structure of a putative yfit-like metal-dependent hydrolase2 (bh0186) from bacillus halodurans c-125 at 2.30 a resolution Phyre2 PDB 1rxq chain A Region: 3 - 134 Aligned: 132 Modelled: 132 Confidence: 98.1% Identity: 14% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: YfiT-like putative metal-dependent hydrolases Phyre2 PDB 2ou6 chain A domain 1 Region: 1 - 129 Aligned: 129 Modelled: 129 Confidence: 98.1% Identity: 15% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 5civ chain A Region: 15 - 103 Aligned: 89 Modelled: 89 Confidence: 98.1% Identity: 18% PDB header:unknown function Chain: A: PDB Molecule:sibling bacteriocin; PDBTitle: sibling lethal factor precursor - dfsb Phyre2 PDB 3dka chain A Region: 15 - 142 Aligned: 128 Modelled: 128 Confidence: 97.9% Identity: 9% PDB header:unknown function Chain: A: PDB Molecule:dinb-like protein; PDBTitle: crystal structure of a dinb-like protein (yjoa, bsu12410) from2 bacillus subtilis at 2.30 a resolution Phyre2 PDB 3cex chain B Region: 16 - 129 Aligned: 114 Modelled: 111 Confidence: 97.8% Identity: 14% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of the conserved protein of locus ef_3021 from2 enterococcus faecalis Phyre2 PDB 3di5 chain A Region: 15 - 141 Aligned: 127 Modelled: 127 Confidence: 97.7% Identity: 11% PDB header:metal binding protein Chain: A: PDB Molecule:dinb-like protein; PDBTitle: crystal structure of a dinb-like protein (bce_4655) from bacillus2 cereus atcc 10987 at 2.01 a resolution Phyre2 PDB 4n6c chain B Region: 12 - 78 Aligned: 67 Modelled: 67 Confidence: 97.7% Identity: 9% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of the b1rzq2 protein from streptococcus pneumoniae.2 northeast structural genomics consortium (nesg) target spr36. Phyre2 PDB 2yqy chain B Region: 17 - 136 Aligned: 116 Modelled: 119 Confidence: 97.6% Identity: 15% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:hypothetical protein ttha0303; PDBTitle: crystal structure of tt2238, a four-helix bundle protein Phyre2 PDB 2hkv chain A domain 1 Region: 16 - 143 Aligned: 128 Modelled: 128 Confidence: 97.6% Identity: 11% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 3e4x chain B Region: 16 - 142 Aligned: 127 Modelled: 127 Confidence: 97.4% Identity: 11% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:apc36150; PDBTitle: crystal structure of putative metal-dependent hydrolases2 apc36150 Phyre2 PDB 2p1a chain A domain 1 Region: 15 - 136 Aligned: 122 Modelled: 122 Confidence: 97.2% Identity: 12% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 5cqv chain B Region: 15 - 78 Aligned: 64 Modelled: 64 Confidence: 97.0% Identity: 6% PDB header:unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of uncharacterized protein q8dwv2 from streptococcus2 agalactiae Phyre2 PDB 5cof chain A Region: 5 - 123 Aligned: 118 Modelled: 119 Confidence: 96.9% Identity: 10% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of uncharacterised protein q1r1x2 from escherichia2 coli uti89 Phyre2 PDB 2qe9 chain B Region: 20 - 144 Aligned: 125 Modelled: 125 Confidence: 96.8% Identity: 10% PDB header:hydrolase Chain: B: PDB Molecule:uncharacterized protein yiza; PDBTitle: crystal structure of a putative metal-dependent hydrolase (yiza,2 bsu10800) from bacillus subtilis at 1.90 a resolution Phyre2 PDB 6anr chain A Region: 10 - 78 Aligned: 69 Modelled: 69 Confidence: 96.7% Identity: 14% PDB header:hydrolase Chain: A: PDB Molecule:colibactin self-protection protein clbs; PDBTitle: crystal structure of a self resistance protein clbs from colibactin2 biosynthetic gene cluster Phyre2 1 c2nsfA_ 2 d2nsfa1 3 c6iz2A_ 4 c5cogB_ 5 c2rd9C_ 6 d1rxqa_ 7 d2ou6a1 8 c5civA_ 9 c3dkaA_ 10 c3cexB_ 11 c3di5A_ 12 c4n6cB_ 13 c2yqyB_ 14 d2hkva1 15 c3e4xB_ 16 d2p1aa1 17 c5cqvB_ 18 c5cofA_ 19 c2qe9B_ 20 c6anrA_ 21 22 23 24 25 26 27 28 29 30 31 32