1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	R	A	G	D	G	K	I	R	V	P	A	D	L	D	A	V	T	A	T	G	E	E	D	H	S	E	I	D	G	A	A	V	D	R	I	W	R	A	A	R	H	W	Y	R	A	G	M	H	P	A	I	Q	L	C	I	R	H	H	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?			?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	V	V	L	N	R	A	I	G	H	G	W	G	N	A	P	T	D	E	A	D	A	E	K	I	P	V	T	T	D	T	P	F	C	V	Y	S	A	A	K	A	I	T	A	T	V	V	H	M	L	V	E	R	G	H	F	A	L	D	D
Secondary structure
SS confidence
Disorder																?	?	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		R	V	C	E	Y	L	P	S	Y	T	S	H	G	K	H	R	T	T	I	R	H	V	L	T	H	S	A	G	V	P	F	P	T	G	P	R	P	D	V	R	R	A	D	D	H	E	Y	A	V	E	R	L	G	E	L	R	P	L	Y	R
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		P	G	L	V	H	I	Y	H	A	L	T	W	G	P	L	M	R	E	I	V	Y	A	A	T	G	K	E	I	R	E	I	L	A	T	E	I	L	D	P	L	G	F	R	W	T	N	F	G	V	A	E	R	D	V	P	L	V	A	P	S
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		H	A	T	G	R	Q	L	P	P	V	I	A	A	V	F	R	K	A	I	G	G	T	V	H	E	I	I	P	Y	T	N	T	P	F	F	L	S	T	I	L	P	S	S	N	T	V	S	T	A	N	E	L	S	R	F	M	E	I	L	R
Secondary structure
SS confidence
Disorder						?	?	?	?						?		?				?	?																?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		R	G	G	E	L	D	G	V	R	V	L	S	P	E	T	L	R	G	A	V	T	E	C	R	R	L	R	P	D	F	A	T	G	L	M	P	L	R	W	G	T	G	F	M	L	G	S	A	K	Y	G	P	F	G	R	N	A	P	A	A
Secondary structure
SS confidence
Disorder								?															?			?	?	?	?	?	?	?	?	?															?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.
Sequence		F	G	H	L	G	L	V	N	I	A	V	W	A	D	P	E	R	A	L	S	G	G	L	I	S	S	G	K	P	G	R	D	P	E	A	G	R	Y	G	A	L	L	N	A	I	T	A	E	I	P	R	A	S	S	G
Secondary structure
SS confidence
Disorder																														?	?	?	?	?	?															?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 19%) Alpha helix ( 29%) Beta strand ( 12%) TM helix ( 5%) (View membrane topology prediction)

Rank Aligned region

PDB 4ivk chain A Region: 20 - 409 Aligned: 364 Modelled: 374 Confidence: 100.0% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:carboxylesterases; PDBTitle: crystal structure of a fammily viii carboxylesterase in a complex with2 cephalothin. Phyre2 PDB 5gkv chain A Region: 26 - 409 Aligned: 368 Modelled: 372 Confidence: 100.0% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:esterase a; PDBTitle: crystal structure of a novel penicillin-binding protein (pbp) homolog2 from caulobacter crescentus Phyre2 PDB 4p6b chain B Region: 34 - 405 Aligned: 356 Modelled: 358 Confidence: 100.0% Identity: 19% PDB header:hydrolase Chain: B: PDB Molecule:est-y29; PDBTitle: crystal structure of est-y29,a novel penicillin-binding protein/beta-2 lactamase homolog from a metagenomic library Phyre2 PDB 3zyt chain A Region: 24 - 409 Aligned: 363 Modelled: 371 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:esterase a; PDBTitle: structure determination of esta from arthrobacter nitroguajacolicus2 rue61a Phyre2 PDB 5gmx chain A Region: 26 - 409 Aligned: 364 Modelled: 376 Confidence: 100.0% Identity: 22% PDB header:hydrolase Chain: A: PDB Molecule:carboxylesterase; PDBTitle: crystal structure of a family viii carboxylesterase Phyre2 PDB 3hle chain A Region: 23 - 411 Aligned: 366 Modelled: 375 Confidence: 100.0% Identity: 19% PDB header:transferase Chain: A: PDB Molecule:transesterase; PDBTitle: simvastatin synthase (lovd), from aspergillus terreus, s5 mutant, s76a2 mutant, complex with monacolin j acid Phyre2 PDB 2qmi chain H Region: 33 - 412 Aligned: 339 Modelled: 360 Confidence: 100.0% Identity: 20% PDB header:hydrolase Chain: H: PDB Molecule:pbp related beta-lactamase; PDBTitle: structure of the octameric penicillin-binding protein2 homologue from pyrococcus abyssi Phyre2 PDB 1ci9 chain A Region: 29 - 407 Aligned: 361 Modelled: 379 Confidence: 100.0% Identity: 20% Fold: beta-lactamase/transpeptidase-like Superfamily: beta-lactamase/transpeptidase-like Family: beta-Lactamase/D-ala carboxypeptidase Phyre2 PDB 3ozh chain A Region: 34 - 407 Aligned: 335 Modelled: 347 Confidence: 100.0% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:beta-lactamase/d-alanine carboxypeptidase; PDBTitle: crystal structure of beta-lactamase/d-alanine carboxypeptidase from2 yersinia pestis Phyre2 PDB 1rgy chain A Region: 26 - 405 Aligned: 332 Modelled: 355 Confidence: 100.0% Identity: 18% Fold: beta-lactamase/transpeptidase-like Superfamily: beta-lactamase/transpeptidase-like Family: beta-Lactamase/D-ala carboxypeptidase Phyre2 PDB 5tgf chain B Region: 34 - 406 Aligned: 311 Modelled: 316 Confidence: 100.0% Identity: 23% PDB header:hydrolase Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of putative beta-lactamase from bacteroides dorei2 dsm 17855 Phyre2 PDB 1onh chain A Region: 27 - 405 Aligned: 334 Modelled: 357 Confidence: 100.0% Identity: 17% Fold: beta-lactamase/transpeptidase-like Superfamily: beta-lactamase/transpeptidase-like Family: beta-Lactamase/D-ala carboxypeptidase Phyre2 PDB 2hds chain A domain 1 Region: 34 - 405 Aligned: 334 Modelled: 348 Confidence: 100.0% Identity: 18% Fold: beta-lactamase/transpeptidase-like Superfamily: beta-lactamase/transpeptidase-like Family: beta-Lactamase/D-ala carboxypeptidase Phyre2 PDB 3ws1 chain A Region: 32 - 405 Aligned: 337 Modelled: 343 Confidence: 100.0% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:beta-lactamase; PDBTitle: n288q-n321q mutant beta-lactamase derived from chromohalobacter sp.5602 (condition-1b) Phyre2 PDB 2wzz chain A Region: 33 - 405 Aligned: 337 Modelled: 350 Confidence: 100.0% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:beta-lactamase; PDBTitle: amp-c beta-lactamase (pseudomonas aeruginosa)in complex2 with compound m-03 Phyre2 PDB 1ei5 chain A Region: 33 - 414 Aligned: 328 Modelled: 361 Confidence: 100.0% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:d-aminopeptidase; PDBTitle: crystal structure of a d-aminopeptidase from ochrobactrum2 anthropi Phyre2 PDB 5e2h chain B Region: 30 - 405 Aligned: 335 Modelled: 340 Confidence: 100.0% Identity: 20% PDB header:hydrolase Chain: B: PDB Molecule:beta-lactamase; PDBTitle: crystal structure of d-alanine carboxypeptidase ampc from2 mycobacterium smegmatis Phyre2 PDB 2drw chain A domain 1 Region: 34 - 414 Aligned: 354 Modelled: 381 Confidence: 100.0% Identity: 20% Fold: beta-lactamase/transpeptidase-like Superfamily: beta-lactamase/transpeptidase-like Family: beta-Lactamase/D-ala carboxypeptidase Phyre2 PDB 5zh8 chain B Region: 17 - 411 Aligned: 333 Modelled: 375 Confidence: 100.0% Identity: 17% PDB header:hydrolase Chain: B: PDB Molecule:protein fmta; PDBTitle: crystal structure of fmta from staphylococcus aureus at 2.58 a Phyre2 PDB 2qz6 chain A Region: 34 - 405 Aligned: 324 Modelled: 340 Confidence: 100.0% Identity: 16% PDB header:hydrolase Chain: A: PDB Molecule:beta-lactamase; PDBTitle: first crystal structure of a psychrophile class c beta-2 lactamase Phyre2 1 c4ivkA_ 2 c5gkvA_ 3 c4p6bB_ 4 c3zytA_ 5 c5gmxA_ 6 c3hleA_ 7 c2qmiH_ 8 d1ci9a_ 9 c3ozhA_ 10 d1rgya_ 11 c5tgfB_ 12 d1onha_ 13 d2hdsa1 14 c3ws1A_ 15 c2wzzA_ 16 c1ei5A_ 17 c5e2hB_ 18 d2drwa1 19 c5zh8B_ 20 c2qz6A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186