1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	Q	V	T	S	V	G	H	A	G	F	L	I	Q	T	Q	A	G	S	I	L	C	D	P	W	V	N	P	A	Y	F	A	S	W	F	P	F	P	D	N	S	G	L	D	W	G	A	L	G	E	C	D	Y	L	Y	V	S	H	L	H
Secondary structure
SS confidence
Disorder		?																												?		?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		K	D	H	F	D	A	E	N	L	R	A	H	V	N	K	D	A	V	V	L	L	P	D	F	P	V	P	D	L	R	N	E	L	Q	K	L	G	F	H	R	F	F	E	T	T	D	S	V	K	H	R	L	R	G	P	N	G	D	L	D
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		V	M	I	I	A	L	R	A	P	A	D	G	P	I	G	D	S	A	L	V	V	A	D	G	E	T	T	A	F	N	M	N	D	A	R	P	V	D	L	D	V	L	A	S	E	F	G	H	I	D	V	H	M	L	Q	Y	S	G	A	I
Secondary structure
SS confidence
Disorder											?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		W	Y	P	M	V	Y	D	M	P	A	R	A	K	D	A	F	G	A	Q	K	R	Q	R	Q	M	D	R	A	R	Q	Y	I	A	Q	V	G	A	T	W	V	V	P	S	A	G	P	P	C	F	L	A	P	E	L	R	H	L	N	D	D
Secondary structure
SS confidence
Disorder																						?	?	?																																					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		G	S	D	P	A	N	I	F	P	D	Q	M	V	F	L	D	Q	M	R	A	H	G	Q	D	G	G	L	L	M	I	P	G	S	T	A	D	F	T	G	T	T	L	N	S	L	R	H	P	L	P	A	E	Q	V	E	A	I	F	T	T
Secondary structure
SS confidence
Disorder					?		?	?		?	?	?	?						?																								?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		D	K	A	A	Y	I	A	D	Y	A	D	R	M	A	P	V	L	A	A	Q	K	A	G	W	A	A	A	A	G	E	P	L	L	Q	P	L	R	T	L	F	E	P	I	M	L	Q	S	N	E	I	C	D	G	I	G	Y	P	V	E	L
Secondary structure
SS confidence
Disorder																												?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		A	I	G	P	E	T	I	V	L	D	F	P	K	R	A	V	R	E	P	I	P	D	E	R	F	R	Y	G	F	A	I	A	P	E	L	V	R	T	V	L	R	D	N	E	P	D	W	V	N	T	I	F	L	S	T	R	F	R	A	W
Secondary structure
SS confidence
Disorder														?	?	?	?	?	?	?																																								?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		R	V	G	G	Y	N	E	Y	L	Y	T	F	F	K	C	L	T	D	E	R	I	A	Y	A	D	G	W	F	A	E	A	H	D	D	S	S	S	I	T	L	N	G	W	E	I	Q	R	R	C	P	H	L	K	A	D	L	S	K	F	G
Secondary structure
SS confidence
Disorder		?	?																															?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.
Sequence		V	V	E	G	N	T	L	T	C	N	L	H	G	W	Q	W	R	L	D	D	G	R	C	L	T	A	R	G	H	Q	L	R	S	S	R	P
Secondary structure
SS confidence
Disorder																															?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 10%) Alpha helix ( 32%) Beta strand ( 25%) TM helix ( 3%) (View membrane topology prediction)

Rank Aligned region

PDB 4jo0 chain A Region: 1 - 277 Aligned: 268 Modelled: 277 Confidence: 100.0% Identity: 19% PDB header:oxidoreductase Chain: A: PDB Molecule:cmla; PDBTitle: crystal structure of cmla, a diiron beta-hydroxylase from streptomyces2 venezuelae Phyre2 PDB 4qn9 chain A Region: 1 - 278 Aligned: 249 Modelled: 262 Confidence: 100.0% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:n-acyl-phosphatidylethanolamine-hydrolyzing phospholipase PDBTitle: structure of human nape-pld Phyre2 PDB 2wyl chain F Region: 1 - 277 Aligned: 239 Modelled: 250 Confidence: 100.0% Identity: 18% PDB header:hydrolase Chain: F: PDB Molecule:l-ascorbate-6-phosphate lactonase ulag; PDBTitle: apo structure of a metallo-b-lactamase Phyre2 PDB 3bv6 chain D Region: 1 - 262 Aligned: 230 Modelled: 237 Confidence: 100.0% Identity: 17% PDB header:hydrolase Chain: D: PDB Molecule:metal-dependent hydrolase; PDBTitle: crystal structure of uncharacterized metallo protein from vibrio2 cholerae with beta-lactamase like fold Phyre2 PDB 3rpc chain D Region: 1 - 277 Aligned: 243 Modelled: 248 Confidence: 100.0% Identity: 13% PDB header:hydrolase Chain: D: PDB Molecule:possible metal-dependent hydrolase; PDBTitle: the crystal structure of a possible metal-dependent hydrolase from2 veillonella parvula dsm 2008 Phyre2 PDB 6brm chain C Region: 1 - 279 Aligned: 245 Modelled: 253 Confidence: 100.0% Identity: 16% PDB header:hydrolase Chain: C: PDB Molecule:putative metal-dependent isothiocyanate hydrolase saxa; PDBTitle: the crystal structure of isothiocyanate hydrolase from delia radicum2 gut bacteria Phyre2 PDB 3kl7 chain A Region: 1 - 262 Aligned: 195 Modelled: 205 Confidence: 99.9% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:putative metal-dependent hydrolase; PDBTitle: crystal structure of putative metal-dependent hydrolase2 (yp_001302908.1) from parabacteroides distasonis atcc 8503 at 2.30 a3 resolution Phyre2 PDB 1vjn chain A Region: 2 - 240 Aligned: 149 Modelled: 168 Confidence: 99.9% Identity: 27% Fold: Metallo-hydrolase/oxidoreductase Superfamily: Metallo-hydrolase/oxidoreductase Family: Hypothetical protein TM0207 Phyre2 PDB 3x30 chain A Region: 1 - 277 Aligned: 222 Modelled: 237 Confidence: 99.9% Identity: 21% PDB header:hydrolase Chain: A: PDB Molecule:upf0173 metal-dependent hydrolase tm_1162; PDBTitle: crystal structure of metallo-beta-lactamase from thermotoga maritima Phyre2 PDB 4xww chain A Region: 1 - 343 Aligned: 325 Modelled: 335 Confidence: 99.6% Identity: 13% PDB header:rna binding protein/rna Chain: A: PDB Molecule:dr2417; PDBTitle: crystal structure of rnase j complexed with rna Phyre2 PDB 3g1p chain A Region: 1 - 222 Aligned: 187 Modelled: 208 Confidence: 99.6% Identity: 18% PDB header:lyase Chain: A: PDB Molecule:protein phnp; PDBTitle: crystals structure of phnp from e.coli k-12 Phyre2 PDB 2i7t chain A domain 1 Region: 1 - 223 Aligned: 196 Modelled: 210 Confidence: 99.6% Identity: 13% Fold: Metallo-hydrolase/oxidoreductase Superfamily: Metallo-hydrolase/oxidoreductase Family: beta-CASP RNA-metabolising hydrolases Phyre2 PDB 1zkp chain D Region: 1 - 274 Aligned: 220 Modelled: 241 Confidence: 99.6% Identity: 17% PDB header:structural genomics, unknown function Chain: D: PDB Molecule:hypothetical protein ba1088; PDBTitle: 1.5a resolution crystal structure of a metallo beta lactamase family2 protein, the elac homolgue of bacillus anthracis, a putative3 ribonuclease Phyre2 PDB 6i1d chain A Region: 1 - 223 Aligned: 198 Modelled: 210 Confidence: 99.5% Identity: 18% PDB header:gene regulation Chain: A: PDB Molecule:endoribonuclease ysh1; PDBTitle: structure of the ysh1-mpe1 nuclease complex from s.cerevisiae Phyre2 PDB 5a0t chain A Region: 1 - 343 Aligned: 321 Modelled: 323 Confidence: 99.5% Identity: 13% PDB header:hydrolase/rna Chain: A: PDB Molecule:ribonuclease j; PDBTitle: catalysis and 5' end sensing by ribonuclease rnase j of the2 metallo-beta-lactamase family Phyre2 PDB 3zwf chain A Region: 1 - 221 Aligned: 201 Modelled: 208 Confidence: 99.5% Identity: 15% PDB header:hydrolase Chain: A: PDB Molecule:zinc phosphodiesterase elac protein 1; PDBTitle: crystal structure of human trnase z, short form (elac1). Phyre2 PDB 3zq4 chain C Region: 6 - 341 Aligned: 316 Modelled: 328 Confidence: 99.5% Identity: 14% PDB header:hydrolase Chain: C: PDB Molecule:ribonuclease j 1; PDBTitle: unusual, dual endo- and exo-nuclease activity in the degradosome2 explained by crystal structure analysis of rnase j1 Phyre2 PDB 6b9v chain A Region: 1 - 277 Aligned: 212 Modelled: 232 Confidence: 99.5% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:beta-lactamase-like protein; PDBTitle: crystal structure of a new diphosphatase from the phnp family Phyre2 PDB 3bk2 chain A Region: 6 - 343 Aligned: 310 Modelled: 317 Confidence: 99.4% Identity: 12% PDB header:hydrolase Chain: A: PDB Molecule:metal dependent hydrolase; PDBTitle: crystal structure analysis of the rnase j/ump complex Phyre2 PDB 1zkp chain A domain 1 Region: 1 - 274 Aligned: 220 Modelled: 244 Confidence: 99.4% Identity: 16% Fold: Metallo-hydrolase/oxidoreductase Superfamily: Metallo-hydrolase/oxidoreductase Family: YhfI-like Phyre2 1 c4jo0A_ 2 c4qn9A_ 3 c2wylF_ 4 c3bv6D_ 5 c3rpcD_ 6 c6brmC_ 7 c3kl7A_ 8 d1vjna_ 9 c3x30A_ 10 c4xwwA_ 11 c3g1pA_ 12 d2i7ta1 13 c1zkpD_ 14 c6i1dA_ 15 c5a0tA_ 16 c3zwfA_ 17 c3zq4C_ 18 c6b9vA_ 19 c3bk2A_ 20 d1zkpa1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201