1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	R	I	G	P	V	E	L	S	A	V	K	D	W	D	P	A	P	G	V	L	V	S	W	H	P	T	P	A	S	C	A	K	A	L	A	A	P	V	S	A	V	P	P	S	Y	V	Q	A	R	Q	I	R	S	F	S	E	Q	A	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																			?	?	?	?	?	?	?	?	?	?	?	?	?	?	?																		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	G	L	D	H	S	R	L	L	I	A	S	V	E	V	F	G	H	C	D	L	R	A	M	T	Y	V	I	N	A	H	L	R	R	H	D	T	Y	R	S	W	F	E	L	R	D	T	D	H	I	V	R	H	S	I	A	D	P	A	D
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		I	E	F	V	P	T	T	H	G	E	M	T	S	A	D	L	R	Q	H	I	V	A	T	P	D	S	L	H	W	D	C	F	S	F	G	V	I	Q	R	A	D	S	F	T	F	Y	A	S	I	D	H	L	H	A	D	G	Q	F	V	G
Secondary structure
SS confidence
Disorder									?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		V	G	L	M	E	F	Q	S	M	Y	T	A	L	I	M	G	E	P	P	I	G	L	S	E	A	G	S	Y	V	D	F	C	V	R	Q	H	E	Y	T	S	A	L	T	V	D	S	P	E	V	R	A	W	I	D	F	A	E	I	N	N
Secondary structure
SS confidence
Disorder																?		?	?	?																				?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		G	T	F	P	E	F	P	L	P	L	G	D	P	S	V	R	C	G	G	D	L	L	S	M	M	L	M	D	E	Q	Q	T	Q	R	F	E	S	A	C	M	A	A	N	A	R	F	I	G	G	M	L	A	C	I	A	I	A	I	H	E
Secondary structure
SS confidence
Disorder																		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	T	G	A	D	T	Y	F	G	I	T	P	K	D	I	R	T	P	A	D	L	M	T	Q	G	W	F	T	G	Q	I	P	V	T	V	P	V	A	G	L	S	F	N	E	I	A	R	I	A	Q	T	S	F	D	T	G	A	D	L	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		K	V	P	F	E	R	V	V	E	L	S	P	S	L	R	R	P	Q	P	L	F	S	L	V	N	F	F	D	A	Q	V	G	P	L	S	A	V	T	K	L	F	E	G	L	N	V	G	T	Y	S	D	G	R	V	T	Y	P	L	S	T
Secondary structure
SS confidence
Disorder																																?	?		?	?	?	?	?	?	?								?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		M	V	G	R	F	D	E	T	A	A	S	V	L	F	P	D	N	P	V	A	R	E	S	V	T	A	Y	L	R	A	I	R	S	V	C	M	R	I	A	N	G	G	T	A	E	R	V	G	N	V	V	A	L	S	P	G	R	R	N	N
Secondary structure
SS confidence
Disorder																																															?	?	?				?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.
Sequence		I	E	R	M	T	W	R	S	C	R	A	G	D	F	I	D	I	C	N	L	K	V	A	N	V	T	V	D	R	E	A
Secondary structure
SS confidence
Disorder																								?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 14%) Alpha helix ( 39%) Beta strand ( 18%) TM helix ( 3%) (View membrane topology prediction)

Rank Aligned region

PDB 6aef chain B Region: 6 - 461 Aligned: 455 Modelled: 455 Confidence: 100.0% Identity: 51% PDB header:transferase Chain: B: PDB Molecule:polyketide synthase associated protein papa2; PDBTitle: papa2 acyl transferase Phyre2 PDB 6n8e chain A Region: 37 - 511 Aligned: 463 Modelled: 475 Confidence: 100.0% Identity: 12% PDB header:hydrolase Chain: A: PDB Molecule:holo-obif1; PDBTitle: crystal structure of holo-obif1, a five domain nonribosomal peptide2 synthetase from burkholderia diffusa Phyre2 PDB 2vsq chain A Region: 34 - 510 Aligned: 461 Modelled: 477 Confidence: 100.0% Identity: 15% PDB header:ligase Chain: A: PDB Molecule:surfactin synthetase subunit 3; PDBTitle: structure of surfactin a synthetase c (srfa-c), a nonribosomal peptide2 synthetase termination module Phyre2 PDB 6p1j chain A Region: 40 - 511 Aligned: 466 Modelled: 472 Confidence: 100.0% Identity: 17% PDB header:biosynthetic protein Chain: A: PDB Molecule:txo2; PDBTitle: the structure of condensation and adenylation domains of teixobactin-2 producing nonribosomal peptide synthetase txo2 serine module Phyre2 PDB 5u89 chain A Region: 27 - 489 Aligned: 447 Modelled: 463 Confidence: 100.0% Identity: 15% PDB header:hydrolase/inhibitor Chain: A: PDB Molecule:amino acid adenylation domain protein; PDBTitle: crystal structure of a cross-module fragment from the dimodular nrps2 dhbf Phyre2 PDB 4zxi chain A Region: 30 - 509 Aligned: 467 Modelled: 479 Confidence: 100.0% Identity: 13% PDB header:biosynthetic protein Chain: A: PDB Molecule:tyrocidine synthetase 3; PDBTitle: crystal structure of holo-ab3403 a four domain nonribosomal peptide2 synthetase bound to amp and glycine Phyre2 PDB 5ja2 chain A Region: 58 - 511 Aligned: 445 Modelled: 451 Confidence: 100.0% Identity: 13% PDB header:ligase Chain: A: PDB Molecule:enterobactin synthase component f; PDBTitle: entf, a terminal nonribosomal peptide synthetase module bound to the2 non-native mbth-like protein pa2412 Phyre2 PDB 4zxj chain A Region: 42 - 511 Aligned: 460 Modelled: 470 Confidence: 100.0% Identity: 13% PDB header:biosynthetic protein Chain: A: PDB Molecule:enterobactin synthase component f; PDBTitle: crystal structure of holo-entf a nonribosomal peptide synthetase in2 the thioester-forming conformation Phyre2 PDB 6ozv chain A Region: 43 - 511 Aligned: 446 Modelled: 454 Confidence: 100.0% Identity: 15% PDB header:biosynthetic protein Chain: A: PDB Molecule:txo1; PDBTitle: the structure of condensation and adenylation domains of teixobactin-2 producing nonribosomal peptide synthetase txo1 serine module in3 complex with amp Phyre2 PDB 4tx3 chain B Region: 27 - 475 Aligned: 439 Modelled: 449 Confidence: 100.0% Identity: 15% PDB header:oxidoreductase Chain: B: PDB Molecule:peptide synthetase, module 7; PDBTitle: complex of the x-domain and oxyb from teicoplanin biosynthesis Phyre2 PDB 6m7l chain B Region: 41 - 472 Aligned: 417 Modelled: 429 Confidence: 100.0% Identity: 14% PDB header:biosynthetic protein Chain: B: PDB Molecule:putative non-ribosomal peptide synthetase; PDBTitle: complex of oxya with the x-domain from gpa biosynthesis Phyre2 PDB 5t81 chain A Region: 27 - 466 Aligned: 431 Modelled: 440 Confidence: 100.0% Identity: 14% PDB header:biosynthetic protein Chain: A: PDB Molecule:epob; PDBTitle: rhombohedral crystal form of the epob nrps cyclization-docking2 bidomain from sorangium cellulosum Phyre2 PDB 2jgp chain A Region: 25 - 473 Aligned: 434 Modelled: 449 Confidence: 100.0% Identity: 16% PDB header:ligase Chain: A: PDB Molecule:tyrocidine synthetase 3; PDBTitle: structure of the tycc5-6 pcp-c bidomain of the tyrocidine synthetase2 tycc Phyre2 PDB 6cgo chain B Region: 28 - 466 Aligned: 428 Modelled: 439 Confidence: 100.0% Identity: 14% PDB header:biosynthetic protein Chain: B: PDB Molecule:condensation domain protein; PDBTitle: molecular basis for condensation domain-mediated chain release from2 the enacyloxin polyketide synthase Phyre2 PDB 4jn3 chain B Region: 35 - 473 Aligned: 423 Modelled: 436 Confidence: 100.0% Identity: 14% PDB header:biosynthetic protein Chain: B: PDB Molecule:cda peptide synthetase i; PDBTitle: crystal structures of the first condensation domain of the cda2 synthetase Phyre2 PDB 2xhg chain A Region: 38 - 462 Aligned: 416 Modelled: 425 Confidence: 100.0% Identity: 13% PDB header:isomerase Chain: A: PDB Molecule:tyrocidine synthetase a; PDBTitle: crystal structure of the epimerization domain from the initiation2 module of tyrocidine biosynthesis Phyre2 PDB 4znm chain B Region: 43 - 464 Aligned: 416 Modelled: 422 Confidence: 100.0% Identity: 16% PDB header:ligase Chain: B: PDB Molecule:c-domain type ii peptide synthetase; PDBTitle: crystal structure of sgcc5 protein from streptomyces globisporus (apo2 form) Phyre2 PDB 5t3e chain A Region: 39 - 463 Aligned: 415 Modelled: 425 Confidence: 100.0% Identity: 10% PDB header:ligase Chain: A: PDB Molecule:bacillamide synthetase heterocyclization domain; PDBTitle: crystal structure of a nonribosomal peptide synthetase2 heterocyclization domain. Phyre2 PDB 6ad3 chain A Region: 36 - 465 Aligned: 419 Modelled: 429 Confidence: 100.0% Identity: 14% PDB header:biosynthetic protein Chain: A: PDB Molecule:lovastatin nonaketide synthase moka; PDBTitle: structural characterization of the condensation domain from monacolin2 k polyketide synthase moka Phyre2 PDB 5m6p chain B Region: 31 - 465 Aligned: 426 Modelled: 435 Confidence: 100.0% Identity: 13% PDB header:isomerase Chain: B: PDB Molecule:tyrocidine synthase 2; PDBTitle: crystal structure of the epimerization domain from module 3 of2 tyrocidine synthetase b, tycb3(e) Phyre2 1 c6aefB_ 2 c6n8eA_ 3 c2vsqA_ 4 c6p1jA_ 5 c5u89A_ 6 c4zxiA_ 7 c5ja2A_ 8 c4zxjA_ 9 c6ozvA_ 10 c4tx3B_ 11 c6m7lB_ 12 c5t81A_ 13 c2jgpA_ 14 c6cgoB_ 15 c4jn3B_ 16 c2xhgA_ 17 c4znmB_ 18 c5t3eA_ 19 c6ad3A_ 20 c5m6pB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68