1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		L	M	A	R	F	E	V	P	E	G	W	C	V	Q	A	F	R	F	T	L	D	P	T	E	D	Q	A	R	A	L	A	R	H	F	G	A	R	R	K	A	Y	N	W	A	V	A	T	L	K	A	D	I	E	A	W	R	V	T	G	I
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	T	V	K	P	S	L	R	V	L	R	K	R	W	N	T	V	K	D	E	V	C	V	N	A	E	T	G	A	V	W	W	P	E	C	S	K	E	A	Y	A	D	G	I	G	G	A	V	D	A	Y	W	N	W	Q	N	S	R	S	G
Secondary structure
SS confidence
Disorder																																																											?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		K	R	E	G	K	T	M	G	F	P	R	F	K	K	K	G	R	D	Q	D	R	V	T	F	T	T	G	A	M	R	V	E	P	D	R	R	H	L	T	L	P	V	V	G	T	V	R	T	H	E	N	T	R	R	I	E	R	L	I	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																																															?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		T	G	R	A	R	V	L	A	I	S	V	R	R	N	G	T	R	L	D	A	S	V	R	V	L	V	Q	R	P	Q	Q	P	N	V	A	Q	P	G	S	R	V	G	V	D	V	G	V	R	R	L	A	T	V	A	N	E	A	G	A	V
Secondary structure
SS confidence
Disorder		?	?																												?					?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	E	E	V	P	N	P	R	P	L	D	T	A	L	K	E	L	R	Y	A	S	R	A	R	S	R	C	T	K	G	S	R	R	Y	R	E	R	T	T	E	I	S	R	L	H	R	R	V	N	D	V	R	T	H	H	L	H	V	L	T
Secondary structure
SS confidence
Disorder																											?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		T	R	L	A	Q	T	H	G	H	I	V	V	E	G	L	D	A	A	G	M	L	R	Q	K	G	L	P	G	A	R	A	R	R	R	G	L	S	D	S	A	L	G	T	P	R	R	H	L	S	Y	K	T	G	W	Y	G	S	A	L	V
Secondary structure
SS confidence
Disorder																						?	?	?	?	?	?	?	?	?	?	?		?					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.
Sequence		V	A	D	R	W	F	P	S	L	S	V	E	P	T	V	R	P	G	L	A	R	L	V	A	V	K	R	G	R	E	A	A	A	W	L	P	N	N	P	E	T	G	C	K	S	R	D	H
Secondary structure
SS confidence
Disorder															?																												?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 13%) Alpha helix ( 38%) Beta strand ( 19%)

Rank Aligned region

PDB 6e7a chain Y Region: 199 - 394 Aligned: 172 Modelled: 180 Confidence: 98.5% Identity: 21% PDB header:dna binding protein/dna/rna Chain: Y: PDB Molecule:casx; PDBTitle: casx-grna-dna(30bp) state ii Phyre2 PDB 5id6 chain A Region: 196 - 375 Aligned: 167 Modelled: 174 Confidence: 97.9% Identity: 16% PDB header:hydrolase Chain: A: PDB Molecule:cpf1; PDBTitle: structure of cpf1/rna complex Phyre2 PDB 6iv6 chain A Region: 196 - 375 Aligned: 164 Modelled: 180 Confidence: 97.7% Identity: 19% PDB header:immune system/rna Chain: A: PDB Molecule:nuclease; PDBTitle: cryo-em structure of acrva5-acetylated mbcas12a in complex with crrna Phyre2 PDB 5b43 chain A Region: 196 - 375 Aligned: 145 Modelled: 157 Confidence: 97.0% Identity: 20% PDB header:hydrolase/rna/dna Chain: A: PDB Molecule:crispr-associated endonuclease cpf1; PDBTitle: crystal structure of acidaminococcus sp. cpf1 in complex with crrna2 and target dna Phyre2 PDB 5nfv chain A Region: 220 - 379 Aligned: 136 Modelled: 145 Confidence: 96.9% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:crispr-associated endonuclease cpf1; PDBTitle: crystal structure of catalytically inactive fncas12 mutant bound to an2 r-loop structure containing a pre-crrna mimic and full-length dna3 target Phyre2 PDB 5mga chain A Region: 196 - 379 Aligned: 155 Modelled: 167 Confidence: 96.8% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:crispr-associated endonuclease cpf1; PDBTitle: structure of the cpf1 endonuclease r-loop complex after dna cleavage Phyre2 PDB 5wti chain Z Region: 284 - 407 Aligned: 120 Modelled: 124 Confidence: 94.6% Identity: 17% PDB header:hydrolase/dna/rna Chain: Z: PDB Molecule:crispr-associated protein; PDBTitle: crystal structure of the crispr-associated protein in complex with2 crrna and dna Phyre2 PDB 1hjr chain A Region: 220 - 364 Aligned: 100 Modelled: 112 Confidence: 94.1% Identity: 17% Fold: Ribonuclease H-like motif Superfamily: Ribonuclease H-like Family: RuvC resolvase Phyre2 PDB 1iv0 chain A Region: 219 - 369 Aligned: 96 Modelled: 107 Confidence: 91.2% Identity: 22% Fold: Ribonuclease H-like motif Superfamily: Ribonuclease H-like Family: Putative Holliday junction resolvase RuvX Phyre2 PDB 1kcf chain B Region: 213 - 233 Aligned: 21 Modelled: 21 Confidence: 89.2% Identity: 14% PDB header:hydrolase Chain: B: PDB Molecule:hypothetical 30.2 kd protein c25g10.02 in PDBTitle: crystal structure of the yeast mitochondrial holliday2 junction resolvase, ydc2 Phyre2 PDB 1kcf chain A domain 2 Region: 217 - 233 Aligned: 17 Modelled: 17 Confidence: 86.1% Identity: 18% Fold: Ribonuclease H-like motif Superfamily: Ribonuclease H-like Family: Mitochondrial resolvase ydc2 catalytic domain Phyre2 PDB 4u3e chain A Region: 340 - 391 Aligned: 47 Modelled: 52 Confidence: 85.5% Identity: 9% PDB header:oxidoreductase Chain: A: PDB Molecule:ribonucleoside triphosphate reductase; PDBTitle: anaerobic ribonucleotide reductase Phyre2 PDB 1hk8 chain A Region: 344 - 393 Aligned: 46 Modelled: 48 Confidence: 84.4% Identity: 7% Fold: PFL-like glycyl radical enzymes Superfamily: PFL-like glycyl radical enzymes Family: Class III anaerobic ribonucleotide reductase NRDD subunit Phyre2 PDB 1hk8 chain A Region: 344 - 393 Aligned: 46 Modelled: 48 Confidence: 84.4% Identity: 7% PDB header:oxidoreductase Chain: A: PDB Molecule:anaerobic ribonucleotide-triphosphate reductase; PDBTitle: structural basis for allosteric substrate specificity regulation in2 class iii ribonucleotide reductases: nrdd in complex with dgtp Phyre2 PDB 5ijl chain A Region: 367 - 408 Aligned: 42 Modelled: 42 Confidence: 83.7% Identity: 17% PDB header:transferase Chain: A: PDB Molecule:dna polymerase ii large subunit; PDBTitle: d-family dna polymerase - dp2 subunit (catalytic subunit) Phyre2 PDB 4ep5 chain A Region: 220 - 364 Aligned: 92 Modelled: 102 Confidence: 83.2% Identity: 14% PDB header:hydrolase Chain: A: PDB Molecule:crossover junction endodeoxyribonuclease ruvc; PDBTitle: thermus thermophilus ruvc structure Phyre2 PDB 5wqe chain A Region: 284 - 378 Aligned: 91 Modelled: 95 Confidence: 82.3% Identity: 15% PDB header:rna binding protein Chain: A: PDB Molecule:crispr-associated endonuclease c2c1; PDBTitle: crystal structure of alicyclobacillus acidoterrestris c2c1 in complex2 with single-guide rna at 3.1 angstrom resolution Phyre2 PDB 5u31 chain A Region: 284 - 390 Aligned: 103 Modelled: 107 Confidence: 79.9% Identity: 16% PDB header:hydrolase/dna Chain: A: PDB Molecule:crispr-associated endonuclease c2c1; PDBTitle: crystal structure of aacc2c1-sgrna-8mer substrate dna ternary complex Phyre2 PDB 2qa4 chain Z Region: 370 - 399 Aligned: 30 Modelled: 30 Confidence: 79.6% Identity: 10% PDB header:ribosome Chain: Z: PDB Molecule:50s ribosomal protein l37ae; PDBTitle: a more complete structure of the the l7/l12 stalk of the2 haloarcula marismortui 50s large ribosomal subunit Phyre2 PDB 3j21 chain I Region: 370 - 399 Aligned: 30 Modelled: 30 Confidence: 79.5% Identity: 27% PDB header:ribosome Chain: I: PDB Molecule:50s ribosomal protein l13p; PDBTitle: promiscuous behavior of proteins in archaeal ribosomes revealed by2 cryo-em: implications for evolution of eukaryotic ribosomes (50s3 ribosomal proteins) Phyre2 1 c6e7aY_ 2 c5id6A_ 3 c6iv6A_ 4 c5b43A_ 5 c5nfvA_ 6 c5mgaA_ 7 c5wtiZ_ 8 d1hjra_ 9 d1iv0a_ 10 c1kcfB_ 11 d1kcfa2 12 c4u3eA_ 13 d1hk8a_ 14 c1hk8A_ 15 c5ijlA_ 16 c4ep5A_ 17 c5wqeA_ 18 c5u31A_ 19 c2qa4Z_ 20 c3j21i_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204