1

.

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10

.

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20

.

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30

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40

.

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50

.

.

.

.

.

.

.

.

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60

Sequence

V

S

T

W

H

R

I

G

T

E

G

E

P

L

T

D

P

L

T

T

Q

A

I

A

A

L

S

R

G

H

G

L

F

A

G

G

V

S

G

A

D

I

D

A

P

Q

I

Q

Q

Y

A

N

A

I

S

W

V

A

N

A

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

70

.

.

.

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.

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.

.

80

.

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90

.

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100

.

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.

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110

.

.

.

.

.

.

.

.

.

120

Sequence

V

P

T

A

A

A

Y

R

W

R

G

A

A

R

A

L

R

R

L

A

N

T

D

E

A

L

A

Q

I

M

A

A

A

Q

I

D

H

A

H

A

R

T

A

T

R

A

L

L

E

A

A

K

T

D

A

M

A

L

T

D

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

130

.

.

.

.

.

.

.

.

.

140

.

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.

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150

.

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.

.

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160

.

.

.

.

.

.

.

.

.

170

.

.

.

.

.

.

.

.

.

180

Sequence

T

P

L

G

R

R

E

A

M

A

R

M

A

A

R

L

R

A

Q

H

R

H

I

A

R

C

R

S

R

A

R

L

L

G

L

R

L

R

R

L

R

Y

L

R

T

A

A

A

R

R

P

Q

V

T

T

P

G

G

R

A

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

190

.

.

.

.

.

.

.

.

.

200

.

.

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.

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.

.

.

.

210

.

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.

.

.

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.

220

.

.

.

.

.

.

.

.

.

230

.

.

.

.

.

.

.

.

.

240

Sequence

Q

V

L

A

A

I

Q

K

A

L

D

I

Q

G

V

H

D

P

A

A

R

A

R

W

T

R

G

M

D

L

V

A

R

R

E

S

N

Y

N

A

N

A

I

N

H

W

D

S

N

A

A

R

G

T

P

S

R

G

V

W

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

250

.

.

.

.

.

.

.

.

.

260

.

.

.

.

.

.

.

.

.

270

.

.

.

.

.

.

.

.

.

280

.

.

.

.

.

.

.

.

.

290

.

.

.

.

.

.

.

.

.

300

Sequence

Q

F

I

A

P

T

F

A

A

Y

H

E

P

G

T

S

T

N

I

H

D

L

V

A

Q

A

C

A

F

I

N

Y

A

R

G

H

Y

G

V

A

A

D

A

S

N

L

A

D

L

I

Q

Q

A

D

P

R

R

S

P

R

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

Sequence

G

Y

Secondary structure

SS confidence

Disorder

?

?

Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 56%) Alpha helix ( 61%) Beta strand ( 1%)

Rank Aligned region

PDB 4cfo chain B Region: 208 - 274 Aligned: 59 Modelled: 67 Confidence: 99.2% Identity: 19% PDB header:hydrolase Chain: B: PDB Molecule:mltc; PDBTitle: structure of lytic transglycosylase mltc from escherichia2 coli in complex with tetrasaccharide at 2.9 a resolution. Phyre2 PDB 2y8p chain A Region: 184 - 274 Aligned: 80 Modelled: 91 Confidence: 99.1% Identity: 18% PDB header:lyase Chain: A: PDB Molecule:endo-type membrane-bound lytic murein transglycosylase a; PDBTitle: crystal structure of an outer membrane-anchored endolytic2 peptidoglycan lytic transglycosylase (mlte) from3 escherichia coli Phyre2 PDB 3w6d chain B Region: 208 - 275 Aligned: 60 Modelled: 68 Confidence: 99.1% Identity: 18% PDB header:hydrolase Chain: B: PDB Molecule:lysozyme-like chitinolytic enzyme; PDBTitle: crystal structure of catalytic domain of chitinase from ralstonia sp.2 a-471 (e141q) in complex with tetrasaccharide Phyre2 PDB 4oz9 chain A Region: 208 - 277 Aligned: 58 Modelled: 70 Confidence: 99.1% Identity: 21% PDB header:lyase Chain: A: PDB Molecule:membrane-bound lytic murein transglycosylase f; PDBTitle: crystal structure of mltf from pseudomonas aeruginosa complexed with2 isoleucine Phyre2 PDB 1qsa chain A domain 2 Region: 187 - 277 Aligned: 80 Modelled: 91 Confidence: 99.0% Identity: 16% Fold: Lysozyme-like Superfamily: Lysozyme-like Family: Bacterial muramidase, catalytic domain Phyre2 PDB 1sly chain A Region: 208 - 275 Aligned: 59 Modelled: 68 Confidence: 99.0% Identity: 19% PDB header:glycosyltransferase Chain: A: PDB Molecule:70-kda soluble lytic transglycosylase; PDBTitle: complex of the 70-kda soluble lytic transglycosylase with2 bulgecin a Phyre2 PDB 6cfc chain A Region: 208 - 275 Aligned: 60 Modelled: 68 Confidence: 99.0% Identity: 20% PDB header:hydrolase Chain: A: PDB Molecule:lytic transglycosylase; PDBTitle: crystal structure of soluble lytic transglycosylase cj0843 of2 campylobacter jejuni in complex with bulgecin a Phyre2 PDB 1gbs chain A Region: 208 - 275 Aligned: 57 Modelled: 68 Confidence: 99.0% Identity: 14% Fold: Lysozyme-like Superfamily: Lysozyme-like Family: G-type lysozyme Phyre2 PDB 3mgw chain A Region: 182 - 275 Aligned: 80 Modelled: 94 Confidence: 98.9% Identity: 14% PDB header:hydrolase Chain: A: PDB Molecule:lysozyme g; PDBTitle: thermodynamics and structure of a salmon cold-active goose-type2 lysozyme Phyre2 PDB 6fcq chain A Region: 208 - 275 Aligned: 60 Modelled: 68 Confidence: 98.9% Identity: 17% PDB header:lyase Chain: A: PDB Molecule:soluble lytic murein transglycosylase; PDBTitle: the x-ray structure of lytic transglycosylase slt inactive mutant2 e503q from pseudomonas aeruginosa in complex with bulgecin a Phyre2 PDB 4xp8 chain A Region: 208 - 277 Aligned: 67 Modelled: 70 Confidence: 98.9% Identity: 13% PDB header:hydrolase Chain: A: PDB Molecule:etga protein; PDBTitle: structure of etga d60n mutant Phyre2 PDB 3gxk chain B Region: 208 - 274 Aligned: 60 Modelled: 67 Confidence: 98.9% Identity: 12% PDB header:hydrolase Chain: B: PDB Molecule:goose-type lysozyme 1; PDBTitle: the crystal structure of g-type lysozyme from atlantic cod (gadus2 morhua l.) in complex with nag oligomers sheds new light on substrate3 binding and the catalytic mechanism. native structure to 1.9 Phyre2 PDB 1xsf chain A Region: 194 - 278 Aligned: 76 Modelled: 85 Confidence: 98.5% Identity: 17% PDB header:cell cycle, hydrolase Chain: A: PDB Molecule:probable resuscitation-promoting factor rpfb; PDBTitle: solution structure of a resuscitation promoting factor2 domain from mycobacterium tuberculosis Phyre2 PDB 1xsf chain A domain 1 Region: 207 - 278 Aligned: 63 Modelled: 72 Confidence: 98.3% Identity: 21% Fold: Lysozyme-like Superfamily: Lysozyme-like Family: RPF-like Phyre2 PDB 4ow1 chain A Region: 208 - 278 Aligned: 62 Modelled: 71 Confidence: 98.3% Identity: 27% PDB header:hydrolase Chain: A: PDB Molecule:resuscitation-promoting factor rpfc; PDBTitle: crystal structure of resuscitation promoting factor c Phyre2 PDB 4hpe chain A Region: 184 - 277 Aligned: 70 Modelled: 81 Confidence: 98.0% Identity: 13% PDB header:hydrolase Chain: A: PDB Molecule:putative cell wall hydrolase tn916-like,ctn1-orf17; PDBTitle: crystal structure of a putative cell wall hydrolase (cd630_03720) from2 clostridium difficile 630 at 2.38 a resolution Phyre2 PDB 3eo5 chain A Region: 208 - 278 Aligned: 62 Modelled: 71 Confidence: 97.8% Identity: 21% PDB header:cell adhesion Chain: A: PDB Molecule:resuscitation-promoting factor rpfb; PDBTitle: crystal structure of the resuscitation promoting factor rpfb Phyre2 PDB 4fdy chain A Region: 184 - 293 Aligned: 85 Modelled: 86 Confidence: 97.7% Identity: 14% PDB header:hydrolase Chain: A: PDB Molecule:similar to lipoprotein, nlp/p60 family; PDBTitle: crystal structure of a similar to lipoprotein, nlp/p60 family2 (sav0400) from staphylococcus aureus subsp. aureus mu50 at 2.23 a3 resolution Phyre2 PDB 5e27 chain B Region: 196 - 278 Aligned: 74 Modelled: 83 Confidence: 97.6% Identity: 23% PDB header:cell adhesion Chain: B: PDB Molecule:resuscitation-promoting factor rpfb; PDBTitle: the structure of resuscitation promoting factor b from m. tuberculosis2 reveals unexpected ubiquitin-like domains Phyre2 PDB 3bkh chain A Region: 208 - 275 Aligned: 61 Modelled: 68 Confidence: 97.5% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:lytic transglycosylase; PDBTitle: crystal structure of the bacteriophage phikz lytic2 transglycosylase, gp144 Phyre2 1 c4cfoB_ 2 c2y8pA_ 3 c3w6dB_ 4 c4oz9A_ 5 d1qsaa2 6 c1slyA_ 7 c6cfcA_ 8 d1gbsa_ 9 c3mgwA_ 10 c6fcqA_ 11 c4xp8A_ 12 c3gxkB_ 13 c1xsfA_ 14 d1xsfa1 15 c4ow1A_ 16 c4hpeA_ 17 c3eo5A_ 18 c4fdyA_ 19 c5e27B_ 20 c3bkhA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58