1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	P	E	A	V	Q	E	A	D	L	L	T	A	A	A	V	A	L	N	R	H	A	A	L	L	R	E	L	G	S	V	F	A	A	A	G	H	E	L	Y	L	V	G	G	S	V	R	D	A	L	L	G	R	L	S	P	D	L	D	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		T	T	D	A	R	P	E	R	V	Q	E	I	V	R	P	W	A	D	A	V	W	D	T	G	I	E	F	G	T	V	G	V	G	K	S	D	H	R	M	E	I	T	T	F	R	A	D	S	Y	D	R	V	S	R	H	P	E	V	R	F
Secondary structure
SS confidence
Disorder																						?																											?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	D	C	L	E	G	D	L	V	R	R	D	F	T	T	N	A	M	A	V	R	V	T	A	T	G	P	G	E	F	L	D	P	L	G	G	L	A	A	L	R	A	K	V	L	D	T	P	A	A	P	S	G	S	F	G	D	D	P	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		R	M	L	R	A	A	R	F	V	S	Q	L	G	F	A	V	A	P	R	V	R	A	A	I	E	E	M	A	P	Q	L	A	R	I	S	A	E	R	V	A	A	E	L	D	K	L	L	V	G	E	D	P	A	A	G	I	D	L	M	V
Secondary structure
SS confidence
Disorder																																?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		Q	S	G	M	G	A	V	V	L	P	E	I	G	G	M	R	M	A	I	D	E	H	H	Q	H	K	D	V	Y	Q	H	S	L	T	V	L	R	Q	A	I	A	L	E	D	D	G	P	D	L	V	L	R	W	A	A	L	L	H	D	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		G	K	P	A	T	R	R	H	E	P	D	G	G	V	S	F	H	H	H	E	V	V	G	A	K	M	V	R	K	R	M	R	A	L	K	Y	S	K	Q	M	I	D	D	I	S	Q	L	V	Y	L	H	L	R	F	H	G	Y	G	D	G
Secondary structure
SS confidence
Disorder							?	?															?																																			?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		K	W	T	D	S	A	V	R	R	Y	V	T	D	A	G	A	L	L	P	R	L	H	K	L	V	R	A	D	C	T	T	R	N	K	R	R	A	A	R	L	Q	A	S	Y	D	R	L	E	E	R	I	A	E	L	A	A	Q	E	D	L
Secondary structure
SS confidence
Disorder		?	?																													?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		D	R	V	R	P	D	L	D	G	N	Q	I	M	A	V	L	D	I	P	A	G	P	Q	V	G	E	A	W	R	Y	L	K	E	L	R	L	E	R	G	P	L	S	T	E	E	A	T	T	E	L	L	S	W	W	K	S	R	G	N	R
Secondary structure
SS confidence
Disorder																																								?		?	?														?	?	?	?	?
Disorder confidence
Sequence
Secondary structure
SS confidence
Disorder
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 9%) Alpha helix ( 57%) Beta strand ( 6%) TM helix ( 7%) (View membrane topology prediction)

Rank Aligned region

PDB 3wfr chain H Region: 18 - 415 Aligned: 380 Modelled: 398 Confidence: 100.0% Identity: 25% PDB header:transferase/rna Chain: H: PDB Molecule:poly a polymerase; PDBTitle: trna processing enzyme complex 2 Phyre2 PDB 3wfp chain A Region: 18 - 424 Aligned: 390 Modelled: 407 Confidence: 100.0% Identity: 25% PDB header:transferase Chain: A: PDB Molecule:poly a polymerase; PDBTitle: trna processing enzyme (apo form 2) Phyre2 PDB 1miy chain B Region: 18 - 477 Aligned: 390 Modelled: 421 Confidence: 100.0% Identity: 32% PDB header:translation, transferase Chain: B: PDB Molecule:trna cca-adding enzyme; PDBTitle: crystal structure of bacillus stearothermophilus cca-adding enzyme in2 complex with ctp Phyre2 PDB 3wfr chain G Region: 18 - 415 Aligned: 381 Modelled: 398 Confidence: 100.0% Identity: 25% PDB header:transferase/rna Chain: G: PDB Molecule:poly a polymerase; PDBTitle: trna processing enzyme complex 2 Phyre2 PDB 3h37 chain B Region: 14 - 479 Aligned: 406 Modelled: 444 Confidence: 100.0% Identity: 21% PDB header:transferase Chain: B: PDB Molecule:trna nucleotidyl transferase-related protein; PDBTitle: the structure of cca-adding enzyme apo form i Phyre2 PDB 3wfs chain D Region: 18 - 410 Aligned: 379 Modelled: 393 Confidence: 100.0% Identity: 24% PDB header:transferase/rna Chain: D: PDB Molecule:poly a polymerase; PDBTitle: trna processing enzyme complex 3 Phyre2 PDB 6q52 chain A Region: 19 - 478 Aligned: 366 Modelled: 414 Confidence: 100.0% Identity: 30% PDB header:rna binding protein Chain: A: PDB Molecule:cca-adding enzyme; PDBTitle: structure of a psychrophilic cca-adding enzyme in complex with cmpcpp2 at room temperature in chipx microfluidic device Phyre2 PDB 4x4w chain B Region: 15 - 473 Aligned: 384 Modelled: 414 Confidence: 100.0% Identity: 25% PDB header:rna binding protein Chain: B: PDB Molecule:cca trna nucleotidyltransferase 1, mitochondrial; PDBTitle: crystal structure of the full-length human mitochondrial cca-adding2 enzyme Phyre2 PDB 3wfq chain H Region: 18 - 416 Aligned: 375 Modelled: 399 Confidence: 100.0% Identity: 26% PDB header:transferase/rna Chain: H: PDB Molecule:poly a polymerase; PDBTitle: trna processing enzyme complex 1 Phyre2 PDB 3wfp chain B Region: 18 - 415 Aligned: 375 Modelled: 398 Confidence: 100.0% Identity: 26% PDB header:transferase Chain: B: PDB Molecule:poly a polymerase; PDBTitle: trna processing enzyme (apo form 2) Phyre2 PDB 3wfq chain E Region: 18 - 416 Aligned: 374 Modelled: 399 Confidence: 100.0% Identity: 26% PDB header:transferase/rna Chain: E: PDB Molecule:poly a polymerase; PDBTitle: trna processing enzyme complex 1 Phyre2 PDB 3wfr chain F Region: 18 - 410 Aligned: 375 Modelled: 393 Confidence: 100.0% Identity: 25% PDB header:transferase/rna Chain: F: PDB Molecule:poly a polymerase; PDBTitle: trna processing enzyme complex 2 Phyre2 PDB 3wfq chain G Region: 18 - 410 Aligned: 374 Modelled: 393 Confidence: 100.0% Identity: 25% PDB header:transferase/rna Chain: G: PDB Molecule:poly a polymerase; PDBTitle: trna processing enzyme complex 1 Phyre2 PDB 3wfr chain E Region: 18 - 410 Aligned: 374 Modelled: 393 Confidence: 100.0% Identity: 25% PDB header:transferase/rna Chain: E: PDB Molecule:poly a polymerase; PDBTitle: trna processing enzyme complex 2 Phyre2 PDB 3wfs chain C Region: 18 - 410 Aligned: 372 Modelled: 393 Confidence: 100.0% Identity: 25% PDB header:transferase/rna Chain: C: PDB Molecule:poly a polymerase; PDBTitle: trna processing enzyme complex 3 Phyre2 PDB 3wfo chain B Region: 18 - 419 Aligned: 382 Modelled: 402 Confidence: 100.0% Identity: 25% PDB header:transferase Chain: B: PDB Molecule:poly a polymerase; PDBTitle: trna processing enzyme (apo form 1) Phyre2 PDB 1vfg chain B Region: 28 - 443 Aligned: 355 Modelled: 384 Confidence: 100.0% Identity: 22% PDB header:transferase/rna Chain: B: PDB Molecule:poly a polymerase; PDBTitle: crystal structure of trna nucleotidyltransferase complexed2 with a primer trna and an incoming atp analog Phyre2 PDB 3aqn chain A Region: 27 - 405 Aligned: 354 Modelled: 379 Confidence: 100.0% Identity: 23% PDB header:transferase Chain: A: PDB Molecule:poly(a) polymerase; PDBTitle: complex structure of bacterial protein (apo form ii) Phyre2 PDB 1ou5 chain A Region: 9 - 392 Aligned: 327 Modelled: 360 Confidence: 100.0% Identity: 25% PDB header:translation, transferase Chain: A: PDB Molecule:trna cca-adding enzyme; PDBTitle: crystal structure of human cca-adding enzyme Phyre2 PDB 1miw chain A domain 1 Region: 164 - 477 Aligned: 260 Modelled: 275 Confidence: 100.0% Identity: 25% Fold: Poly A polymerase C-terminal region-like Superfamily: Poly A polymerase C-terminal region-like Family: Poly A polymerase C-terminal region-like Phyre2 1 c3wfrH_ 2 c3wfpA_ 3 c1miyB_ 4 c3wfrG_ 5 c3h37B_ 6 c3wfsD_ 7 c6q52A_ 8 c4x4wB_ 9 c3wfqH_ 10 c3wfpB_ 11 c3wfqE_ 12 c3wfrF_ 13 c3wfqG_ 14 c3wfrE_ 15 c3wfsC_ 16 c3wfoB_ 17 c1vfgB_ 18 c3aqnA_ 19 c1ou5A_ 20 d1miwa1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131