1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	P	S	P	R	R	E	D	G	D	A	L	R	C	G	D	R	S	A	A	V	T	E	I	R	A	A	L	T	A	L	G	M	L	D	H	Q	E	E	D	L	T	T	G	R	N	V	A	L	E	L	F	D	A	Q	L	D	Q	A	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?				?		?																						?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	A	F	Q	Q	H	R	G	L	L	V	D	G	I	V	G	E	A	T	Y	R	A	L	K	E	A	S	Y	R	L	G	A	R	T	L	Y	H	Q	F	G	A	P	L	Y	G	D	D	V	A	T	L	Q	A	R	L	Q	D	L	G	F
Secondary structure
SS confidence
Disorder																											?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Y	T	G	L	V	D	G	H	F	G	L	Q	T	H	N	A	L	M	S	Y	Q	R	E	Y	G	L	A	A	D	G	I	C	G	P	E	T	L	R	S	L	Y	F	L	S	S	R	V	S	G	G	S	P	H	A	I	R	E	E	E	L
Secondary structure
SS confidence
Disorder																									?															?		?	?	?	?		?	?	?	?	?	?	?	?	?		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		V	R	S	S	G	P	K	L	S	G	K	R	I	I	I	D	P	G	R	G	G	V	D	H	G	L	I	A	Q	G	P	A	G	P	I	S	E	A	D	L	L	W	D	L	A	S	R	L	E	G	R	M	A	A	I	G	M	E	T	H
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	S	R	P	T	N	R	S	P	S	D	A	E	R	A	A	T	A	N	A	V	G	A	D	L	M	I	S	L	R	C	E	T	Q	T	S	L	A	A	N	G	V	A	S	F	H	F	G	N	S	H	G	S	V	S	T	I	G	R	N
Secondary structure
SS confidence
Disorder																																																			?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	A	D	F	I	Q	R	E	V	V	A	R	T	G	L	R	D	C	R	V	H	G	R	T	W	D	L	L	R	L	T	R	M	P	T	V	Q	V	D	I	G	Y	I	T	N	P	H	D	R	G	M	L	V	S	T	Q	T	R	D	A
Secondary structure
SS confidence
Disorder														?		?																																					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.
Sequence		I	A	E	G	I	L	A	A	V	K	R	L	Y	L	L	G	K	N	D	R	P	T	G	T	F	T	F	A	E	L	L	A	H	E	L	S	V	E	R	A	G	R	L	G	G	S
Secondary structure
SS confidence
Disorder																?	?	?	?	?	?	?	?	?	?	?	?							?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 26%) Alpha helix ( 38%) Beta strand ( 10%) TM helix ( 4%) (View membrane topology prediction)

Rank Aligned region

PDB 4bin chain A Region: 183 - 373 Aligned: 188 Modelled: 191 Confidence: 100.0% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:n-acetylmuramoyl-l-alanine amidase amic; PDBTitle: crystal structure of the e. coli n-acetylmuramoyl-l-alanine amidase2 amic Phyre2 PDB 5j72 chain B Region: 150 - 373 Aligned: 211 Modelled: 215 Confidence: 100.0% Identity: 21% PDB header:hydrolase Chain: B: PDB Molecule:putative n-acetylmuramoyl-l-alanine amidase,autolysin cwp6; PDBTitle: cwp6 from clostridium difficile Phyre2 PDB 1jwq chain A Region: 191 - 373 Aligned: 174 Modelled: 183 Confidence: 100.0% Identity: 23% Fold: Phosphorylase/hydrolase-like Superfamily: Zn-dependent exopeptidases Family: N-acetylmuramoyl-L-alanine amidase-like Phyre2 PDB 3ne8 chain A Region: 189 - 373 Aligned: 182 Modelled: 185 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:n-acetylmuramoyl-l-alanine amidase; PDBTitle: the crystal structure of a domain from n-acetylmuramoyl-l-alanine2 amidase of bartonella henselae str. houston-1 Phyre2 PDB 4rn7 chain A Region: 190 - 373 Aligned: 178 Modelled: 184 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:n-acetylmuramoyl-l-alanine amidase; PDBTitle: the crystal structure of n-acetylmuramoyl-l-alanine amidase from2 clostridium difficile 630 Phyre2 PDB 5emi chain A Region: 189 - 375 Aligned: 176 Modelled: 187 Confidence: 100.0% Identity: 26% PDB header:hydrolase Chain: A: PDB Molecule:cell wall hydrolase/autolysin; PDBTitle: n-acetylmuramoyl-l-alanine amidase amic2 of nostoc punctiforme Phyre2 PDB 4m6i chain A Region: 188 - 373 Aligned: 176 Modelled: 186 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:peptidoglycan amidase rv3717; PDBTitle: structure of the reduced, zn-bound form of mycobacterium tuberculosis2 peptidoglycan amidase rv3717 Phyre2 PDB 1xov chain A Region: 191 - 381 Aligned: 178 Modelled: 191 Confidence: 100.0% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:ply protein; PDBTitle: the crystal structure of the listeria monocytogenes bacteriophage psa2 endolysin plypsa Phyre2 PDB 3czx chain A Region: 189 - 378 Aligned: 174 Modelled: 189 Confidence: 100.0% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:putative n-acetylmuramoyl-l-alanine amidase; PDBTitle: the crystal structure of the putative n-acetylmuramoyl-l-2 alanine amidase from neisseria meningitidis Phyre2 PDB 1xov chain A domain 2 Region: 191 - 369 Aligned: 166 Modelled: 179 Confidence: 100.0% Identity: 17% Fold: Phosphorylase/hydrolase-like Superfamily: Zn-dependent exopeptidases Family: N-acetylmuramoyl-L-alanine amidase-like Phyre2 PDB 3qay chain C Region: 192 - 375 Aligned: 172 Modelled: 184 Confidence: 100.0% Identity: 17% PDB header:lyase Chain: C: PDB Molecule:endolysin; PDBTitle: catalytic domain of cd27l endolysin targeting clostridia difficile Phyre2 PDB 1lbu chain A Region: 11 - 165 Aligned: 147 Modelled: 147 Confidence: 99.7% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:muramoyl-pentapeptide carboxypeptidase; PDBTitle: hydrolase metallo (zn) dd-peptidase Phyre2 PDB 3bkh chain A Region: 95 - 166 Aligned: 72 Modelled: 70 Confidence: 99.7% Identity: 32% PDB header:hydrolase Chain: A: PDB Molecule:lytic transglycosylase; PDBTitle: crystal structure of the bacteriophage phikz lytic2 transglycosylase, gp144 Phyre2 PDB 5tv7 chain A Region: 95 - 166 Aligned: 71 Modelled: 72 Confidence: 99.7% Identity: 38% PDB header:hydrolase Chain: A: PDB Molecule:putative peptidoglycan-binding/hydrolysing protein; PDBTitle: 2.05 angstrom resolution crystal structure of peptidoglycan-binding2 protein from clostridioides difficile in complex with glutamine3 hydroxamate. Phyre2 PDB 5bmq chain A Region: 93 - 196 Aligned: 91 Modelled: 90 Confidence: 99.7% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:erfk/ybis/ycfs/ynhg family protein; PDBTitle: crystal structure of l,d-transpeptidase (yku) from stackebrandtia2 nassauensis Phyre2 PDB 4lpq chain A Region: 94 - 196 Aligned: 86 Modelled: 86 Confidence: 99.7% Identity: 34% PDB header:transferase Chain: A: PDB Molecule:erfk/ybis/ycfs/ynhg family protein; PDBTitle: crystal structure of the l,d-transpeptidase (residues 123-326) from2 xylanimonas cellulosilytica dsm 15894 Phyre2 PDB 1lbu chain A domain 1 Region: 91 - 169 Aligned: 79 Modelled: 79 Confidence: 99.6% Identity: 35% Fold: PGBD-like Superfamily: PGBD-like Family: Peptidoglycan binding domain, PGBD Phyre2 PDB 5nm7 chain A Region: 98 - 162 Aligned: 64 Modelled: 65 Confidence: 99.6% Identity: 36% PDB header:hydrolase Chain: A: PDB Molecule:peptidoglycan-binding domain 1; PDBTitle: crystal structure of burkholderia ap3 phage endolysin Phyre2 PDB 4g54 chain A Region: 93 - 194 Aligned: 100 Modelled: 102 Confidence: 99.6% Identity: 21% PDB header:peptidoglycan-binding protein Chain: A: PDB Molecule:general secretion pathway protein; PDBTitle: crystal structure of a periplasmic domain of the epsab fusion protein2 of the vibrio vulnificus type ii secretion system Phyre2 PDB 4xxt chain A Region: 98 - 161 Aligned: 63 Modelled: 59 Confidence: 99.5% Identity: 32% PDB header:hydrolase Chain: A: PDB Molecule:fusion of predicted zn-dependent amidase/peptidase (cell PDBTitle: crystal structure of fused zn-dependent2 amidase/peptidase/peptodoglycan-binding domain-containing protein3 from clostridium acetobutylicum atcc 824 Phyre2 1 c4binA_ 2 c5j72B_ 3 d1jwqa_ 4 c3ne8A_ 5 c4rn7A_ 6 c5emiA_ 7 c4m6iA_ 8 c1xovA_ 9 c3czxA_ 10 d1xova2 11 c3qayC_ 12 c1lbuA_ 13 c3bkhA_ 14 c5tv7A_ 15 c5bmqA_ 16 c4lpqA_ 17 d1lbua1 18 c5nm7A_ 19 c4g54A_ 20 c4xxtA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126