sgRNA Design Tool for Mycobacteria

Input a target gene or coordinates of a target region and this tool will output candidate sgRNAs for Sth1 dCas9. sgRNA features will be updated as CRISPRi design rules are refined. Current design parameters: sgRNA design 2.0 (release September, 2021).


Mycobacteria Genomes M. tuberculosis H37Rv - accession number: NC_018143.2
M. smegmatis mc2-155 - accession number: NC_008596.1
M. abscessus - accession number: NC_010397.1
M. avium - accession number: CP000479.1

Input
Gene ID: e.g. Rv0667 or RVBD0667 for M. tuberculosis; MSMEG0001 or MSMEG_0001 for M. smegmatis; MAB0013C for M. abscessus; MAV_0021 or MAV0021 for M. avium.
Upstream flanking region: Number of nucleotides before the translational start codon of the query gene. This additional sequence will be included as part of the query.
Downstream flanking region: Number of nucleotides after the translational stop codon of the query gene. This additional sequence will be included as part of the query.



Start coordinate: Coordinates based on the genomes listed above.
End coordinate:

Options