1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	T	L	E	I	L	R	S	G	P	L	A	L	V	E	D	L	G	R	A	G	L	A	H	L	G	V	G	R	S	G	A	A	D	R	R	S	H	T	L	A	N	R	L	V	A	N	P	D	D	W	A	T	V	E	V	T	F	G
Secondary structure
SS confidence
Disorder		?	?	?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	F	S	A	R	V	R	G	G	D	V	D	I	A	V	T	G	A	D	T	D	P	T	V	N	G	I	M	V	G	T	N	S	I	H	H	V	R	D	G	Q	V	I	S	L	G	T	P	R	A	G	L	R	T	Y	L	A	V	R	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	V	C	V	E	P	V	L	G	S	R	S	Y	D	V	M	S	A	I	G	P	S	P	L	R	A	G	D	V	L	P	V	G	E	H	T	D	D	Y	P	E	L	D	Q	A	P	V	A	A	I	E	E	H	L	V	E	L	R	V	V
Secondary structure
SS confidence
Disorder																	?	?					?	?	?													?	?	?	?	?	?	?					?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		P	G	P	R	D	D	W	L	V	D	P	D	A	L	V	H	T	I	W	M	A	S	N	R	S	D	R	V	G	M	R	L	Q	G	R	P	L	Q	H	R	W	P	D	R	Q	L	P	G	E	G	V	T	R	G	A	I	Q	V	P	P
Secondary structure
SS confidence
Disorder															?																							?	?	?	?	?	?	?	?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		N	G	L	P	V	I	L	G	P	D	H	P	I	T	G	S	Y	P	V	V	G	V	I	T	D	E	D	I	D	K	V	A	Q	I	R	P	G	Q	Y	V	R	L	H	W	A	R	P	R	S	R	L	P	G	Q	G	V	T	Q	A	W
Secondary structure
SS confidence
Disorder																																															?													?	?
Disorder confidence
Sequence
Secondary structure
SS confidence
Disorder
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 12%) Alpha helix ( 16%) Beta strand ( 34%)

Rank Aligned region

PDB 3mml chain E Region: 2 - 292 Aligned: 291 Modelled: 291 Confidence: 100.0% Identity: 79% PDB header:hydrolase Chain: E: PDB Molecule:allophanate hydrolase subunit 2; PDBTitle: allophanate hydrolase complex from mycobacterium smegmatis, msmeg0435-2 msmeg0436 Phyre2 PDB 5dud chain A Region: 4 - 300 Aligned: 287 Modelled: 297 Confidence: 100.0% Identity: 34% PDB header:unknown function Chain: A: PDB Molecule:ybgk; PDBTitle: crystal structure of e. coli ybgjk Phyre2 PDB 3oep chain A Region: 2 - 283 Aligned: 270 Modelled: 282 Confidence: 100.0% Identity: 31% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:putative uncharacterized protein ttha0988; PDBTitle: crystal structure of ttha0988 in space group p43212 Phyre2 PDB 3va7 chain A Region: 1 - 300 Aligned: 298 Modelled: 299 Confidence: 100.0% Identity: 28% PDB header:ligase Chain: A: PDB Molecule:klla0e08119p; PDBTitle: crystal structure of the kluyveromyces lactis urea carboxylase Phyre2 PDB 5i8i chain D Region: 2 - 300 Aligned: 297 Modelled: 299 Confidence: 100.0% Identity: 28% PDB header:hydrolase Chain: D: PDB Molecule:urea amidolyase; PDBTitle: crystal structure of the k. lactis urea amidolyase Phyre2 PDB 5vz0 chain D Region: 3 - 282 Aligned: 244 Modelled: 262 Confidence: 99.3% Identity: 10% PDB header:ligase Chain: D: PDB Molecule:pyruvate carboxylase; PDBTitle: crystal structure of lactococcus lactis pyruvate carboxylase g746a2 mutant in complex with cyclic-di-amp Phyre2 PDB 3bg5 chain B Region: 3 - 135 Aligned: 113 Modelled: 120 Confidence: 99.1% Identity: 12% PDB header:ligase Chain: B: PDB Molecule:pyruvate carboxylase; PDBTitle: crystal structure of staphylococcus aureus pyruvate carboxylase Phyre2 PDB 4qsk chain B Region: 3 - 69 Aligned: 67 Modelled: 67 Confidence: 98.9% Identity: 7% PDB header:ligase Chain: B: PDB Molecule:pyruvate carboxylase; PDBTitle: crystal structure of l. monocytogenes pyruvate carboxylase in complex2 with cyclic-di-amp Phyre2 PDB 4hnv chain B Region: 3 - 130 Aligned: 113 Modelled: 128 Confidence: 98.5% Identity: 9% PDB header:ligase Chain: B: PDB Molecule:pyruvate carboxylase; PDBTitle: crystal structure of r54e mutant of s. aureus pyruvate carboxylase Phyre2 PDB 3hbl chain A Region: 3 - 130 Aligned: 108 Modelled: 128 Confidence: 98.3% Identity: 13% PDB header:ligase Chain: A: PDB Molecule:pyruvate carboxylase; PDBTitle: crystal structure of s. aureus pyruvate carboxylase t908a mutant Phyre2 PDB 3tw6 chain B Region: 6 - 58 Aligned: 53 Modelled: 53 Confidence: 97.9% Identity: 11% PDB header:ligase/activator Chain: B: PDB Molecule:pyruvate carboxylase protein; PDBTitle: structure of rhizobium etli pyruvate carboxylase t882a with the2 allosteric activator, acetyl coenzyme-a Phyre2 PDB 2qf7 chain A Region: 9 - 134 Aligned: 115 Modelled: 126 Confidence: 96.3% Identity: 14% PDB header:ligase Chain: A: PDB Molecule:pyruvate carboxylase protein; PDBTitle: crystal structure of a complete multifunctional pyruvate carboxylase2 from rhizobium etli Phyre2 PDB 2phc chain B Region: 212 - 289 Aligned: 74 Modelled: 77 Confidence: 95.0% Identity: 18% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein ph0987; PDBTitle: crystal structure of conserved uncharacterized protein ph0987 from2 pyrococcus horikoshii Phyre2 PDB 2phc chain B domain 1 Region: 212 - 289 Aligned: 74 Modelled: 77 Confidence: 93.9% Identity: 18% Fold: Cyclophilin-like Superfamily: Cyclophilin-like Family: PH0987 C-terminal domain-like Phyre2 PDB 2zp2 chain B Region: 212 - 289 Aligned: 70 Modelled: 77 Confidence: 93.9% Identity: 17% PDB header:transferase inhibitor Chain: B: PDB Molecule:kinase a inhibitor; PDBTitle: c-terminal domain of kipi from bacillus subtilis Phyre2 PDB 5dud chain B Region: 231 - 286 Aligned: 53 Modelled: 56 Confidence: 90.7% Identity: 23% PDB header:unknown function Chain: B: PDB Molecule:ybgj; PDBTitle: crystal structure of e. coli ybgjk Phyre2 PDB 1x7f chain A domain 1 Region: 190 - 282 Aligned: 93 Modelled: 93 Confidence: 90.4% Identity: 15% Fold: Cyclophilin-like Superfamily: Cyclophilin-like Family: Outer surface protein, C-terminal domain Phyre2 PDB 2p0o chain A Region: 242 - 289 Aligned: 48 Modelled: 48 Confidence: 88.5% Identity: 19% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein duf871; PDBTitle: crystal structure of a conserved protein from locus ef_2437 in2 enterococcus faecalis with an unknown function Phyre2 PDB 3els chain A Region: 83 - 118 Aligned: 36 Modelled: 36 Confidence: 88.3% Identity: 17% PDB header:splicing Chain: A: PDB Molecule:pre-mrna leakage protein 1; PDBTitle: crystal structure of yeast pml1p, residues 51-204 Phyre2 PDB 3mml chain D Region: 212 - 285 Aligned: 71 Modelled: 74 Confidence: 87.9% Identity: 24% PDB header:hydrolase Chain: D: PDB Molecule:allophanate hydrolase subunit 1; PDBTitle: allophanate hydrolase complex from mycobacterium smegmatis, msmeg0435-2 msmeg0436 Phyre2 1 c3mmlE_ 2 c5dudA_ 3 c3oepA_ 4 c3va7A_ 5 c5i8iD_ 6 c5vz0D_ 7 c3bg5B_ 8 c4qskB_ 9 c4hnvB_ 10 c3hblA_ 11 c3tw6B_ 12 c2qf7A_ 13 c2phcB_ 14 d2phcb1 15 c2zp2B_ 16 c5dudB_ 17 d1x7fa1 18 c2p0oA_ 19 c3elsA_ 20 c3mmlD_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99