1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		L	S	R	F	T	E	K	M	F	H	N	A	R	T	A	T	T	G	M	V	T	G	E	P	H	M	P	V	R	H	T	W	G	E	V	H	E	R	A	R	C	I	A	G	G	L	A	A	A	G	V	G	L	G	D	V	V	G	V	L
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	G	F	P	V	E	I	A	P	T	A	Q	A	L	W	M	R	G	A	S	L	T	M	L	H	Q	P	T	P	R	T	D	L	A	V	W	A	E	D	T	M	T	V	I	G	M	I	E	A	K	A	V	I	V	S	E	P	F	L	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	I	P	I	L	E	Q	K	G	M	Q	V	L	T	V	A	D	L	L	A	S	D	P	I	G	P	I	E	V	G	E	D	D	L	A	L	M	Q	L	T	S	G	S	T	G	S	P	K	A	V	Q	I	T	H	R	N	I	Y	S	N
Secondary structure
SS confidence
Disorder																									?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	E	A	M	F	V	G	A	Q	Y	D	V	D	K	D	V	M	V	S	W	L	P	C	F	H	D	M	G	M	V	G	F	L	T	I	P	M	F	F	G	A	E	L	V	K	V	T	P	M	D	F	L	R	D	T	L	L	W	A	K
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	I	D	K	Y	Q	G	T	M	T	A	A	P	N	F	A	Y	A	L	L	A	K	R	L	R	R	Q	A	K	P	G	D	F	D	L	S	T	L	R	F	A	L	S	G	A	E	P	V	E	P	A	D	V	E	D	L	L	D	A	G
Secondary structure
SS confidence
Disorder																														?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		K	P	F	G	L	R	P	S	A	I	L	P	A	Y	G	M	A	E	T	T	L	A	V	S	F	S	E	C	N	A	G	L	V	V	D	E	V	D	A	D	L	L	A	A	L	R	R	A	V	P	A	T	K	G	N	T	R	R	L	A
Secondary structure
SS confidence
Disorder																																								?				?	?	?	?		?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		T	L	G	P	L	L	Q	D	L	E	A	R	I	I	D	E	Q	G	D	V	M	P	A	R	G	V	G	V	I	E	L	R	G	E	S	L	T	P	G	Y	L	T	M	G	G	F	I	P	A	Q	D	E	H	G	W	Y	D	T	G	D
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		L	G	Y	L	T	E	E	G	H	V	V	V	C	G	R	V	K	D	V	I	I	M	A	G	R	N	I	Y	P	T	D	I	E	R	A	A	G	R	V	D	G	V	R	P	G	C	A	V	A	V	R	L	D	A	G	H	S	R	E	S
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		F	A	V	A	V	E	S	N	A	F	E	D	P	A	E	V	R	R	I	E	H	Q	V	A	H	E	V	V	A	E	V	D	V	R	P	R	N	V	V	V	L	G	P	G	T	I	P	K	T	P	S	G	K	L	R	R	A	N	S	V
Secondary structure
SS confidence
Disorder									?	?	?	?	?																																?	?		?	?
Disorder confidence
		.	.	.	.
Sequence		T	L	V	T
Secondary structure
SS confidence
Disorder				?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 6%) Alpha helix ( 35%) Beta strand ( 26%) TM helix ( 7%) (View membrane topology prediction)

Rank Aligned region

PDB 6eqo chain B Region: 5 - 543 Aligned: 507 Modelled: 517 Confidence: 100.0% Identity: 21% PDB header:oxidoreductase Chain: B: PDB Molecule:acetyl-coenzyme a synthetase; PDBTitle: tri-functional propionyl-coa synthase of erythrobacter sp. nap1 with2 bound nadp+ and phosphomethylphosphonic acid adenylate ester Phyre2 PDB 5gxd chain A Region: 4 - 543 Aligned: 508 Modelled: 518 Confidence: 100.0% Identity: 19% PDB header:lyase Chain: A: PDB Molecule:amp-dependent synthetase and ligase; PDBTitle: structure of acryloyl-coa lyase prpe from dinoroseobacter shibae dfl2 12 Phyre2 PDB 6p1j chain A Region: 1 - 539 Aligned: 487 Modelled: 515 Confidence: 100.0% Identity: 20% PDB header:biosynthetic protein Chain: A: PDB Molecule:txo2; PDBTitle: the structure of condensation and adenylation domains of teixobactin-2 producing nonribosomal peptide synthetase txo2 serine module Phyre2 PDB 5es8 chain A Region: 4 - 543 Aligned: 470 Modelled: 504 Confidence: 100.0% Identity: 19% PDB header:ligase Chain: A: PDB Molecule:linear gramicidin synthetase subunit a; PDBTitle: crystal structure of the initiation module of lgra in the thiolation2 state Phyre2 PDB 1pg4 chain A Region: 3 - 543 Aligned: 507 Modelled: 517 Confidence: 100.0% Identity: 18% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 5ifi chain A Region: 4 - 543 Aligned: 502 Modelled: 516 Confidence: 100.0% Identity: 18% PDB header:ligase Chain: A: PDB Molecule:acetyl-coenzyme a synthetase; PDBTitle: crystal structure of acetyl-coa synthetase in complex with adenosine-2 5'-propylphosphate from cryptococcus neoformans h99 Phyre2 PDB 5wmm chain A Region: 1 - 539 Aligned: 468 Modelled: 493 Confidence: 100.0% Identity: 22% PDB header:biosynthetic protein Chain: A: PDB Molecule:nrps; PDBTitle: crystal structure of an adenylation domain interrupted by a2 methylation domain (ama4) from nonribosomal peptide synthetase tios Phyre2 PDB 4wd1 chain A Region: 4 - 543 Aligned: 499 Modelled: 515 Confidence: 100.0% Identity: 19% PDB header:ligase Chain: A: PDB Molecule:acetoacetate-coa ligase; PDBTitle: acetoacetyl-coa synthetase from streptomyces lividans Phyre2 PDB 5u89 chain A Region: 4 - 544 Aligned: 474 Modelled: 500 Confidence: 100.0% Identity: 23% PDB header:hydrolase/inhibitor Chain: A: PDB Molecule:amino acid adenylation domain protein; PDBTitle: crystal structure of a cross-module fragment from the dimodular nrps2 dhbf Phyre2 PDB 1ry2 chain A Region: 4 - 543 Aligned: 503 Modelled: 517 Confidence: 100.0% Identity: 19% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 5ja2 chain A Region: 3 - 542 Aligned: 489 Modelled: 521 Confidence: 100.0% Identity: 20% PDB header:ligase Chain: A: PDB Molecule:enterobactin synthase component f; PDBTitle: entf, a terminal nonribosomal peptide synthetase module bound to the2 non-native mbth-like protein pa2412 Phyre2 PDB 2vsq chain A Region: 3 - 542 Aligned: 482 Modelled: 506 Confidence: 100.0% Identity: 21% PDB header:ligase Chain: A: PDB Molecule:surfactin synthetase subunit 3; PDBTitle: structure of surfactin a synthetase c (srfa-c), a nonribosomal peptide2 synthetase termination module Phyre2 PDB 3gqw chain B Region: 1 - 542 Aligned: 530 Modelled: 542 Confidence: 100.0% Identity: 28% PDB header:ligase Chain: B: PDB Molecule:fatty acid amp ligase; PDBTitle: crystal structure of a fatty acid amp ligase from e. coli with an acyl2 adenylate product bound Phyre2 PDB 6n8e chain A Region: 2 - 539 Aligned: 489 Modelled: 517 Confidence: 100.0% Identity: 21% PDB header:hydrolase Chain: A: PDB Molecule:holo-obif1; PDBTitle: crystal structure of holo-obif1, a five domain nonribosomal peptide2 synthetase from burkholderia diffusa Phyre2 PDB 5msd chain A Region: 1 - 542 Aligned: 494 Modelled: 512 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: A: PDB Molecule:carboxylic acid reductase; PDBTitle: structure of the a domain of carboxylic acid reductase (car) from2 nocardia iowensis in complex with amp and benzoic acid Phyre2 PDB 3e7w chain A Region: 4 - 543 Aligned: 492 Modelled: 514 Confidence: 100.0% Identity: 17% PDB header:ligase Chain: A: PDB Molecule:d-alanine--poly(phosphoribitol) ligase subunit 1; PDBTitle: crystal structure of dlta: implications for the reaction mechanism of2 non-ribosomal peptide synthetase (nrps) adenylation domains Phyre2 PDB 4wv3 chain A Region: 4 - 542 Aligned: 492 Modelled: 513 Confidence: 100.0% Identity: 18% PDB header:ligase/ligase inhibitor Chain: A: PDB Molecule:anthranilate-coa ligase; PDBTitle: crystal structure of the anthranilate coa ligase auaeii in complex2 with anthranoyl-amp Phyre2 PDB 4zxi chain A Region: 3 - 542 Aligned: 464 Modelled: 506 Confidence: 100.0% Identity: 20% PDB header:biosynthetic protein Chain: A: PDB Molecule:tyrocidine synthetase 3; PDBTitle: crystal structure of holo-ab3403 a four domain nonribosomal peptide2 synthetase bound to amp and glycine Phyre2 PDB 3cw9 chain A domain 1 Region: 1 - 544 Aligned: 499 Modelled: 518 Confidence: 100.0% Identity: 21% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 3kxw chain A Region: 4 - 542 Aligned: 530 Modelled: 539 Confidence: 100.0% Identity: 24% PDB header:ligase Chain: A: PDB Molecule:saframycin mx1 synthetase b; PDBTitle: the crystal structure of fatty acid amp ligase from legionella2 pneumophila Phyre2 1 c6eqoB_ 2 c5gxdA_ 3 c6p1jA_ 4 c5es8A_ 5 d1pg4a_ 6 c5ifiA_ 7 c5wmmA_ 8 c4wd1A_ 9 c5u89A_ 10 d1ry2a_ 11 c5ja2A_ 12 c2vsqA_ 13 c3gqwB_ 14 c6n8eA_ 15 c5msdA_ 16 c3e7wA_ 17 c4wv3A_ 18 c4zxiA_ 19 d3cw9a1 20 c3kxwA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130