1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	L	D	A	V	S	D	A	R	R	D	G	F	A	V	G	E	D	Y	T	V	T	D	R	S	T	G	G	S	R	Q	Q	R	A	A	R	L	G	Q	A	Q	G	H	A	D	F	I	R	H	R	V	G	A	L	L	A	T	D	R	D
Secondary structure
SS confidence
Disorder		?	?	?	?										?										?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	A	T	R	V	S	A	A	T	Q	G	L	D	E	L	A	F	E	D	V	P	G	V	D	T	P	A	E	D	G	V	Q	A	V	D	F	R	Q	A	P	P	P	G	A	P	G	G	M	S	S	G	D	I	D	A	I	D	A	A	N
Secondary structure
SS confidence
Disorder													?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?				?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		R	A	L	L	Q	D	M	L	A	E	Y	S	R	L	P	D	G	Q	V	K	T	D	R	L	A	D	I	A	A	I	Q	E	A	L	R	V	P	D	S	H	L	I	Y	V	A	R	P	D	D	P	A	D	M	I	P	A	V	T	A	V
Secondary structure
SS confidence
Disorder													?	?	?	?	?	?	?																	?	?												?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		G	D	P	F	T	A	D	H	V	S	V	T	V	P	G	V	S	G	T	T	R	Q	T	I	A	T	M	T	Q	E	T	R	G	L	R	E	E	A	R	V	I	A	H	S	V	G	E	S	E	N	V	A	T	I	A	W	V	G	Y	Q
Secondary structure
SS confidence
Disorder																																													?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		P	P	P	V	L	A	S	W	N	T	V	D	D	D	L	A	Q	A	G	A	P	K	L	E	A	F	L	R	D	L	Q	A	G	S	H	N	P	G	H	T	T	A	L	F	G	H	S	Y	G	S	L	L	S	G	I	A	L	K	D	G
Secondary structure
SS confidence
Disorder							?		?	?		?		?																				?		?			?																					?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		A	S	S	L	V	D	N	A	V	L	Y	G	S	P	G	F	D	A	T	S	P	A	K	L	G	M	N	D	H	N	F	F	V	M	T	T	P	D	D	P	I	R	Y	P	A	R	L	A	P	L	H	G	W	G	S	D	G	A	D	T
Secondary structure
SS confidence
Disorder		?	?	?															?						?																												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.
Sequence		I	G	T	V	G	R	Q	G	T	P	A	R	V	G	I	R	P	Q	R	D	H	R	R	I	P	G	P	L	P	L	H	P	S	A	D	R	R	G	I	H	S	A	G
Secondary structure
SS confidence
Disorder		?	?		?	?	?	?			?	?	?	?		?	?	?	?	?	?																?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 28%) Alpha helix ( 38%) Beta strand ( 12%)

Rank Aligned region

PDB 1tca chain A Region: 183 - 314 Aligned: 106 Modelled: 132 Confidence: 97.9% Identity: 25% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Fungal lipases Phyre2 PDB 3icv chain A Region: 183 - 314 Aligned: 105 Modelled: 116 Confidence: 97.7% Identity: 25% PDB header:hydrolase Chain: A: PDB Molecule:lipase b; PDBTitle: structural consequences of a circular permutation on lipase b from2 candida antartica Phyre2 PDB 5x88 chain A Region: 186 - 341 Aligned: 143 Modelled: 156 Confidence: 97.7% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:cutinase; PDBTitle: a crystal structure of cutinases from malbranchea cinnamomea Phyre2 PDB 1cex chain A Region: 186 - 341 Aligned: 143 Modelled: 156 Confidence: 97.7% Identity: 20% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Cutinase-like Phyre2 PDB 3dd5 chain F Region: 186 - 341 Aligned: 144 Modelled: 156 Confidence: 97.6% Identity: 14% PDB header:hydrolase Chain: F: PDB Molecule:cutinase; PDBTitle: glomerella cingulata e600-cutinase complex Phyre2 PDB 3gbs chain A Region: 186 - 341 Aligned: 143 Modelled: 156 Confidence: 97.5% Identity: 16% PDB header:hydrolase Chain: A: PDB Molecule:cutinase 1; PDBTitle: crystal structure of aspergillus oryzae cutinase Phyre2 PDB 1ei9 chain A Region: 191 - 320 Aligned: 113 Modelled: 130 Confidence: 97.5% Identity: 11% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Thioesterases Phyre2 PDB 1qoz chain A Region: 186 - 341 Aligned: 139 Modelled: 153 Confidence: 97.4% Identity: 21% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Cutinase-like Phyre2 PDB 3lp5 chain A Region: 204 - 314 Aligned: 109 Modelled: 111 Confidence: 97.3% Identity: 15% PDB header:hydrolase Chain: A: PDB Molecule:putative cell surface hydrolase; PDBTitle: the crystal structure of the putative cell surface hydrolase from2 lactobacillus plantarum wcfs1 Phyre2 PDB 1g66 chain A Region: 186 - 341 Aligned: 139 Modelled: 154 Confidence: 97.1% Identity: 17% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Cutinase-like Phyre2 PDB 1isp chain A Region: 187 - 314 Aligned: 102 Modelled: 128 Confidence: 97.1% Identity: 15% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Bacterial lipase Phyre2 PDB 3bdv chain B Region: 191 - 341 Aligned: 115 Modelled: 116 Confidence: 96.9% Identity: 21% PDB header:hydrolase Chain: B: PDB Molecule:uncharacterized protein duf1234; PDBTitle: crystal structure of a putative yden-like hydrolase (eca3091) from2 pectobacterium atrosepticum scri1043 at 1.66 a resolution Phyre2 PDB 3aja chain A Region: 184 - 341 Aligned: 147 Modelled: 158 Confidence: 96.9% Identity: 20% PDB header:hydrolase Chain: A: PDB Molecule:putative uncharacterized protein; PDBTitle: crystal structure of msmeg_6394 Phyre2 PDB 1tia chain A Region: 254 - 347 Aligned: 92 Modelled: 94 Confidence: 96.8% Identity: 23% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Fungal lipases Phyre2 PDB 1ex9 chain A Region: 187 - 325 Aligned: 112 Modelled: 139 Confidence: 96.8% Identity: 16% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Bacterial lipase Phyre2 PDB 1tib chain A Region: 254 - 347 Aligned: 92 Modelled: 94 Confidence: 96.7% Identity: 24% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Fungal lipases Phyre2 PDB 2h1i chain A domain 1 Region: 191 - 341 Aligned: 149 Modelled: 0 Confidence: 96.6% Identity: 14% Fold: alpha/beta-Hydrolases Superfamily: alpha/beta-Hydrolases Family: Carboxylesterase/thioesterase 1 Phyre2 PDB 6cl4 chain A Region: 260 - 317 Aligned: 55 Modelled: 58 Confidence: 96.6% Identity: 22% PDB header:hydrolase Chain: A: PDB Molecule:lipase c12; PDBTitle: lipc12 - lipase from metagenomics Phyre2 PDB 4gw3 chain A Region: 260 - 318 Aligned: 56 Modelled: 59 Confidence: 96.5% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:putative lipase; PDBTitle: crystal structure of the lipase from proteus mirabilis Phyre2 PDB 4psd chain A Region: 186 - 341 Aligned: 134 Modelled: 153 Confidence: 96.5% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:carbohydrate esterase family 5; PDBTitle: structure of trichoderma reesei cutinase native form. Phyre2 1 d1tcaa_ 2 c3icvA_ 3 c5x88A_ 4 d1cexa_ 5 c3dd5F_ 6 c3gbsA_ 7 d1ei9a_ 8 d1qoza_ 9 c3lp5A_ 10 d1g66a_ 11 d1ispa_ 12 c3bdvB_ 13 c3ajaA_ 14 d1tiaa_ 15 d1ex9a_ 16 d1tiba_ 17 d2h1ia1 18 c6cl4A_ 19 c4gw3A_ 20 c4psdA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273