1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	N	R	P	D	I	I	I	V	M	T	D	E	E	R	A	V	P	P	Y	E	S	A	E	V	L	A	W	R	Q	R	S	L	T	G	R	R	W	F	D	E	H	G	I	S	F	T	R	H	Y	T	G	S	L	A	C	V	P	S
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	P	T	I	F	T	G	Q	Y	P	D	L	H	G	V	T	Q	T	D	G	I	G	K	R	F	D	D	S	R	L	R	W	L	R	A	G	E	V	P	T	L	G	N	W	F	R	A	A	G	Y	D	T	H	Y	D	G	K	W	H	I
Secondary structure
SS confidence
Disorder																							?						?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		S	H	A	D	L	E	D	P	A	T	G	A	P	L	A	T	N	D	N	E	G	V	V	D	S	A	A	V	R	R	Y	L	D	A	D	P	L	G	P	Y	G	F	S	G	W	V	G	P	E	P	H	G	A	G	L	A	N	S	G	F
Secondary structure
SS confidence
Disorder					?	?	?	?	?																														?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		R	R	D	P	L	V	A	D	R	V	V	A	W	L	T	E	R	Y	A	R	R	R	A	G	D	T	A	A	M	R	P	F	L	L	V	A	S	F	V	N	P	H	D	I	V	L	F	P	A	W	V	W	R	S	P	L	K	P	S	P
Secondary structure
SS confidence
Disorder																								?	?	?	?	?	?																														?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	D	P	P	H	V	P	A	A	P	T	A	D	E	D	L	S	T	K	P	A	A	Q	V	A	Y	R	E	A	Y	Y	S	G	Y	G	L	T	R	M	V	S	R	N	Y	A	R	N	A	Q	R	Y	R	D	L	Y	Y	R	L	H	A
Secondary structure
SS confidence
Disorder				?																			?	?		?	?	?	?	?	?	?	?			?			?	?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		E	V	D	G	P	I	D	R	V	G	R	A	V	T	E	G	G	S	E	D	A	M	L	V	R	T	S	D	H	G	D	L	L	G	A	H	G	G	L	H	Q	K	W	F	N	L	Y	D	E	A	T	R	V	P	F	V	I	A	R	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		G	E	K	A	T	Q	P	R	T	V	S	A	P	T	S	H	V	D	L	V	P	T	L	L	S	A	A	G	V	D	V	D	V	V	A	A	A	L	A	E	S	F	S	E	V	H	P	L	P	G	R	D	L	M	P	V	V	D	G	A
Secondary structure
SS confidence
Disorder			?		?	?	?	?	?	?																											?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		S	A	D	E	G	R	A	I	Y	L	M	T	R	D	N	V	L	E	G	D	T	G	A	S	L	L	S	R	Q	L	G	R	I	V	N	P	P	A	P	L	R	I	K	V	P	A	H	V	A	A	N	F	E	G	L	V	V	R	V	D
Secondary structure
SS confidence
Disorder																?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		D	T	D	A	A	G	G	A	G	H	L	W	K	L	V	R	T	F	D	D	P	A	T	W	T	E	P	G	V	R	H	L	A	T	N	G	M	G	G	D	A	Y	R	T	D	P	L	D	D	Q	W	E	L	Y	D	L	T	A	D	P
Secondary structure
SS confidence
Disorder			?		?																	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		I	E	A	Y	N	R	W	T	D	P	Q	L	H	E	L	R	Q	H	L	R	M	L	L	K	Q	Q	R	A	V	S	V	P	E	R	N	Q	P	W	P	Y	A	H	R	L	P	P	S	G	A	S	N	G	L	V	R	R	V	L	G	R
Secondary structure
SS confidence
Disorder																													?	?	?	?	?	?	?												?			?
Disorder confidence
		.	.	.
Sequence		F	V	R
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 14%) Alpha helix ( 30%) Beta strand ( 7%) TM helix ( 3%) (View membrane topology prediction)

Rank Aligned region

PDB 4upl chain C Region: 5 - 576 Aligned: 467 Modelled: 500 Confidence: 100.0% Identity: 23% PDB header:hydrolase Chain: C: PDB Molecule:sulfatase family protein; PDBTitle: dimeric sulfatase spas2 from silicibacter pomeroyi Phyre2 PDB 4upi chain A Region: 5 - 577 Aligned: 470 Modelled: 476 Confidence: 100.0% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:sulfatase family protein; PDBTitle: dimeric sulfatase spas1 from silicibacter pomeroyi Phyre2 PDB 4uph chain A Region: 4 - 585 Aligned: 492 Modelled: 500 Confidence: 100.0% Identity: 22% PDB header:hydrolase Chain: A: PDB Molecule:sulfatase (sulfuric ester hydrolase) protein; PDBTitle: crystal structure of phosphonate monoester hydrolase of agrobacterium2 radiobacter Phyre2 PDB 4upk chain C Region: 4 - 578 Aligned: 481 Modelled: 495 Confidence: 100.0% Identity: 22% PDB header:hydrolase Chain: C: PDB Molecule:phosphonate monoester hydrolase; PDBTitle: phosphonate monoester hydrolase sppmh from silicibacter pomeroyi Phyre2 PDB 2vqr chain A Region: 4 - 591 Aligned: 497 Modelled: 506 Confidence: 100.0% Identity: 20% PDB header:hydrolase Chain: A: PDB Molecule:putative sulfatase; PDBTitle: crystal structure of a phosphonate monoester hydrolase from rhizobium2 leguminosarum: a new member of the alkaline phosphatase superfamily Phyre2 PDB 4ug4 chain H Region: 1 - 569 Aligned: 441 Modelled: 483 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: H: PDB Molecule:choline sulfatase; PDBTitle: crystal structure of a choline sulfatase from sinorhizobium2 melliloti Phyre2 PDB 1hdh chain A Region: 3 - 571 Aligned: 476 Modelled: 494 Confidence: 100.0% Identity: 18% Fold: Alkaline phosphatase-like Superfamily: Alkaline phosphatase-like Family: Arylsulfatase Phyre2 PDB 4fdi chain A Region: 3 - 586 Aligned: 454 Modelled: 456 Confidence: 100.0% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:n-acetylgalactosamine-6-sulfatase; PDBTitle: the molecular basis of mucopolysaccharidosis iv a Phyre2 PDB 5fql chain A Region: 2 - 559 Aligned: 480 Modelled: 494 Confidence: 100.0% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:iduronate-2-sulfatase; PDBTitle: insights into hunter syndrome from the structure of iduronate-2-2 sulfatase Phyre2 PDB 6hhm chain A Region: 4 - 586 Aligned: 455 Modelled: 476 Confidence: 100.0% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:arylsulfatase; PDBTitle: crystal structure of the family s1_7 ulvan-specific sulfatase fa220702 from formosa agariphila Phyre2 PDB 3ed4 chain A Region: 4 - 592 Aligned: 455 Modelled: 479 Confidence: 100.0% Identity: 20% PDB header:transferase Chain: A: PDB Molecule:arylsulfatase; PDBTitle: crystal structure of putative arylsulfatase from escherichia coli Phyre2 PDB 2qzu chain A Region: 2 - 573 Aligned: 450 Modelled: 464 Confidence: 100.0% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:putative sulfatase yidj; PDBTitle: crystal structure of the putative sulfatase yidj from bacteroides2 fragilis. northeast structural genomics consortium target bfr123 Phyre2 PDB 1p49 chain A Region: 2 - 577 Aligned: 453 Modelled: 476 Confidence: 100.0% Identity: 22% Fold: Alkaline phosphatase-like Superfamily: Alkaline phosphatase-like Family: Arylsulfatase Phyre2 PDB 1auk chain A Region: 3 - 579 Aligned: 447 Modelled: 473 Confidence: 100.0% Identity: 22% Fold: Alkaline phosphatase-like Superfamily: Alkaline phosphatase-like Family: Arylsulfatase Phyre2 PDB 1fsu chain A Region: 2 - 590 Aligned: 465 Modelled: 495 Confidence: 100.0% Identity: 17% Fold: Alkaline phosphatase-like Superfamily: Alkaline phosphatase-like Family: Arylsulfatase Phyre2 PDB 3b5q chain B Region: 3 - 593 Aligned: 447 Modelled: 466 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: B: PDB Molecule:putative sulfatase yidj; PDBTitle: crystal structure of a putative sulfatase (np_810509.1) from2 bacteroides thetaiotaomicron vpi-5482 at 2.40 a resolution Phyre2 PDB 6b1v chain B Region: 3 - 577 Aligned: 425 Modelled: 447 Confidence: 100.0% Identity: 18% PDB header:hydrolase Chain: B: PDB Molecule:iota-carrageenan sulfatase; PDBTitle: crystal structure of ps i-cgsb c78s in complex with i-neocarratetraose Phyre2 PDB 4miv chain B Region: 5 - 587 Aligned: 455 Modelled: 473 Confidence: 100.0% Identity: 20% PDB header:hydrolase Chain: B: PDB Molecule:n-sulphoglucosamine sulphohydrolase; PDBTitle: crystal structure of sulfamidase, crystal form l Phyre2 PDB 5g2v chain A Region: 3 - 573 Aligned: 441 Modelled: 457 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:n-acetylglucosamine-6-sulfatase; PDBTitle: structure of bt4656 in complex with its substrate d-glucosamine-2-n,2 6-o-disulfate. Phyre2 PDB 6j66 chain B Region: 3 - 575 Aligned: 457 Modelled: 475 Confidence: 100.0% Identity: 19% PDB header:hydrolase Chain: B: PDB Molecule:chondroitin sulfate/dermatan sulfate 4-o-endosulfatase PDBTitle: chondroitin sulfate/dermatan sulfate endolytic 4-o-sulfatase Phyre2 1 c4uplC_ 2 c4upiA_ 3 c4uphA_ 4 c4upkC_ 5 c2vqrA_ 6 c4ug4H_ 7 d1hdha_ 8 c4fdiA_ 9 c5fqlA_ 10 c6hhmA_ 11 c3ed4A_ 12 c2qzuA_ 13 d1p49a_ 14 d1auka_ 15 d1fsua_ 16 c3b5qB_ 17 c6b1vB_ 18 c4mivB_ 19 c5g2vA_ 20 c6j66B_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104