1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	M	G	R	V	A	M	L	T	G	S	R	G	L	R	Y	A	T	V	I	A	L	V	A	A	L	V	G	G	V	Y	V	L	S	S	T	G	N	K	R	T	I	V	G	Y	F	T	S	A	V	G	L	Y	P	G	D	Q	V	R	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?																											?	?	?	?	?	?													?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	G	V	P	V	G	E	I	D	M	I	E	P	R	S	S	D	V	K	I	T	M	S	V	S	K	D	V	K	V	P	V	D	V	Q	A	V	I	M	S	P	N	L	V	A	A	R	F	I	Q	L	T	P	V	Y	T	G	G	A	V
Secondary structure
SS confidence
Disorder															?												?				?											?	?	?	?	?							?				?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	P	D	N	G	R	I	D	L	D	R	T	A	V	P	V	E	W	D	E	V	K	E	G	L	T	R	L	A	A	D	L	S	P	A	A	G	E	L	Q	G	P	L	G	A	A	I	N	Q	A	A	D	T	L	D	G	N	G	D	S
Secondary structure
SS confidence
Disorder		?		?	?	?		?	?	?	?	?	?	?	?	?	?	?																?		?	?	?	?	?																?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	H	N	A	L	R	E	L	A	Q	V	A	G	R	L	G	D	S	R	G	D	I	F	G	T	V	K	N	L	Q	V	L	V	D	A	L	S	E	S	D	E	Q	I	V	Q	F	A	G	H	V	A	S	V	S	Q	V	L	A	D	S
Secondary structure
SS confidence
Disorder																			?																																										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		S	A	N	L	D	Q	T	L	G	T	L	N	Q	A	L	S	D	I	R	G	F	L	R	E	N	N	S	T	L	I	E	T	V	N	Q	L	N	D	F	A	Q	T	L	S	D	Q	S	E	N	I	E	Q	V	L	H	V	A	G	P	G
Secondary structure
SS confidence
Disorder																									?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		I	T	N	F	Y	N	I	Y	D	P	A	Q	G	T	L	N	G	L	L	S	I	P	N	F	A	N	P	V	Q	F	I	C	G	G	S	F	D	T	A	A	G	P	S	A	P	D	Y	Y	R	R	A	E	I	C	R	E	R	L	G	P
Secondary structure
SS confidence
Disorder																																			?	?	?	?	?	?	?	?	?	?											?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		V	L	R	R	L	T	V	N	Y	P	P	I	M	F	H	P	L	N	T	I	T	A	Y	K	G	Q	I	I	Y	D	T	P	A	T	E	A	K	S	E	T	P	V	P	E	L	T	W	V	P	A	G	G	G	A	P	V	G	N	P	A
Secondary structure
SS confidence
Disorder				?	?	?	?	?	?	?				?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.
Sequence		D	L	Q	S	L	L	V	P	P	A	P	G	P	A	P	A	P	P	A	P	G	A	G	P	G	E	H	G	G	G	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 34%) Alpha helix ( 45%) Beta strand ( 10%) TM helix ( 4%) (View membrane topology prediction)

Rank Aligned region

PDB 6ic4 chain C Region: 11 - 144 Aligned: 131 Modelled: 134 Confidence: 99.8% Identity: 18% PDB header:protein transport Chain: C: PDB Molecule:toluene tolerance efflux transporter (abc superfamily, PDBTitle: cryo-em structure of the a. baumannii mla complex at 8.7 a resolution Phyre2 PDB 5uw8 chain C Region: 36 - 133 Aligned: 98 Modelled: 98 Confidence: 99.7% Identity: 21% PDB header:transport protein Chain: C: PDB Molecule:probable phospholipid abc transporter-binding protein mlad; PDBTitle: structure of e. coli mce protein mlad, core mce domain Phyre2 PDB 5uvn chain B Region: 36 - 187 Aligned: 139 Modelled: 120 Confidence: 99.4% Identity: 13% PDB header:transport protein Chain: B: PDB Molecule:paraquat-inducible protein b; PDBTitle: structure of e. coli mce protein pqib, periplasmic domain Phyre2 PDB 5uvn chain D Region: 36 - 187 Aligned: 139 Modelled: 120 Confidence: 99.4% Identity: 13% PDB header:transport protein Chain: D: PDB Molecule:paraquat-inducible protein b; PDBTitle: structure of e. coli mce protein pqib, periplasmic domain Phyre2 PDB 5uvn chain C Region: 36 - 187 Aligned: 139 Modelled: 120 Confidence: 99.4% Identity: 13% PDB header:transport protein Chain: C: PDB Molecule:paraquat-inducible protein b; PDBTitle: structure of e. coli mce protein pqib, periplasmic domain Phyre2 PDB 5uvn chain E Region: 36 - 187 Aligned: 139 Modelled: 120 Confidence: 99.4% Identity: 13% PDB header:transport protein Chain: E: PDB Molecule:paraquat-inducible protein b; PDBTitle: structure of e. coli mce protein pqib, periplasmic domain Phyre2 PDB 5uvn chain F Region: 36 - 187 Aligned: 139 Modelled: 120 Confidence: 99.4% Identity: 13% PDB header:transport protein Chain: F: PDB Molecule:paraquat-inducible protein b; PDBTitle: structure of e. coli mce protein pqib, periplasmic domain Phyre2 PDB 5uvn chain A Region: 36 - 187 Aligned: 139 Modelled: 120 Confidence: 99.4% Identity: 13% PDB header:transport protein Chain: A: PDB Molecule:paraquat-inducible protein b; PDBTitle: structure of e. coli mce protein pqib, periplasmic domain Phyre2 PDB 3g67 chain A Region: 137 - 307 Aligned: 171 Modelled: 171 Confidence: 94.9% Identity: 9% PDB header:signaling protein Chain: A: PDB Molecule:methyl-accepting chemotaxis protein; PDBTitle: crystal structure of a soluble chemoreceptor from thermotoga2 maritima Phyre2 PDB 1qu7 chain A Region: 138 - 307 Aligned: 170 Modelled: 170 Confidence: 94.7% Identity: 9% PDB header:signaling protein Chain: A: PDB Molecule:methyl-accepting chemotaxis protein i; PDBTitle: four helical-bundle structure of the cytoplasmic domain of a serine2 chemotaxis receptor Phyre2 PDB 2d4y chain A Region: 161 - 276 Aligned: 116 Modelled: 116 Confidence: 90.3% Identity: 15% PDB header:structural protein Chain: A: PDB Molecule:flagellar hook-associated protein 1; PDBTitle: crystal structure of a 49k fragment of hap1 (flgk) Phyre2 PDB 5u0p chain U Region: 171 - 282 Aligned: 112 Modelled: 112 Confidence: 85.4% Identity: 10% PDB header:transcription Chain: U: PDB Molecule:mediator complex subunit 21; PDBTitle: cryo-em structure of the transcriptional mediator Phyre2 PDB 3lnr chain A Region: 167 - 276 Aligned: 110 Modelled: 110 Confidence: 85.1% Identity: 11% PDB header:signaling protein Chain: A: PDB Molecule:aerotaxis transducer aer2; PDBTitle: crystal structure of poly-hamp domains from the p. aeruginosa soluble2 receptor aer2 Phyre2 PDB 3zx6 chain A Region: 172 - 293 Aligned: 122 Modelled: 122 Confidence: 84.3% Identity: 16% PDB header:signaling Chain: A: PDB Molecule:hamp, methyl-accepting chemotaxis protein i; PDBTitle: structure of hamp(af1503)-tsr fusion - hamp (a291v) mutant Phyre2 PDB 2j5u chain B Region: 61 - 103 Aligned: 43 Modelled: 43 Confidence: 83.8% Identity: 12% PDB header:cell shape regulation Chain: B: PDB Molecule:mrec protein; PDBTitle: mrec lysteria monocytogenes Phyre2 PDB 2ieq chain C Region: 179 - 268 Aligned: 88 Modelled: 90 Confidence: 83.5% Identity: 15% PDB header:viral protein Chain: C: PDB Molecule:spike glycoprotein; PDBTitle: core structure of s2 from the human coronavirus nl63 spike2 glycoprotein Phyre2 PDB 2wpq chain A Region: 161 - 262 Aligned: 98 Modelled: 102 Confidence: 81.8% Identity: 5% PDB header:membrane protein Chain: A: PDB Molecule:trimeric autotransporter adhesin fragment; PDBTitle: salmonella enterica sada 479-519 fused to gcn4 adaptors (sadak3, in-2 register fusion) Phyre2 PDB 2yfa chain A Region: 138 - 297 Aligned: 160 Modelled: 160 Confidence: 81.4% Identity: 10% PDB header:receptor Chain: A: PDB Molecule:methyl-accepting chemotaxis transducer; PDBTitle: x-ray structure of mcps ligand binding domain in complex with malate Phyre2 PDB 2qih chain A Region: 164 - 292 Aligned: 125 Modelled: 129 Confidence: 81.3% Identity: 11% PDB header:cell adhesion Chain: A: PDB Molecule:protein uspa1; PDBTitle: crystal structure of 527-665 fragment of uspa1 protein from moraxella2 catarrhalis Phyre2 PDB 2ch7 chain A Region: 138 - 293 Aligned: 148 Modelled: 156 Confidence: 80.7% Identity: 8% PDB header:chemotaxis Chain: A: PDB Molecule:methyl-accepting chemotaxis protein; PDBTitle: crystal structure of the cytoplasmic domain of a bacterial2 chemoreceptor from thermotoga maritima Phyre2 1 c6ic4C_ 2 c5uw8C_ 3 c5uvnB_ 4 c5uvnD_ 5 c5uvnC_ 6 c5uvnE_ 7 c5uvnF_ 8 c5uvnA_ 9 c3g67A_ 10 c1qu7A_ 11 c2d4yA_ 12 c5u0pU_ 13 c3lnrA_ 14 c3zx6A_ 15 c2j5uB_ 16 c2ieqC_ 17 c2wpqA_ 18 c2yfaA_ 19 c2qihA_ 20 c2ch7A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166