MSMEG0725 (MSMEG0725)

background  +5   0  -5 -10 -15 sgRNA L2FC (+ATc/–ATc) TnSeq Gene Essentiality Predictions (1, 2) Essential Growth Defect Non-Essential Growth Advantage Unknown Operon Legend (3) Operon (1) PMID: 28096490 (2) PMID: 31388080 (3) PMID: 26536359 MSMEG0720 MSMEG0722 MSMEG0723 MSMEG0724 MSMEG0725 MSMEG0726 MSMEG0727 MSMEG0728 MSMEG0729 MSMEG0730
Species: M. smegmatis
Strain: MC2-155
PATRIC Annotation: -
Synonyms:
M. tuberculosis homologs RVBD3475, RVBD3380c, RVBD3326, RVBD3187, RVBD3185, [+ 13 remaining orthologs]
M. abscessus homologs MAB2086c, MAB2088
Gene Start: 812164
Gene End: 813117
Vulnerability Index (VI):
VI Lower Bound:
VI Upper Bound:
[AlphaFold] [Orthologs] [Phyre2] [UniProt] [MycoBrowser] [TnSeq DB]

Essentiality:

Call Condition Type Source Notes
Non Essential in vitro TnSeq [Akusobi et al. (2022)]
Unknown in vitro CRISPRi [Bosch & DeJesus et al. (2021)]


DNA and Protein Sequence help

DNA Sequence

GACGAGCTGAAGGTGCGGGCGGTGCAGATGGTGGCCGATCTGCGCAGCGAGACAGTTTCGGAGTGGGAGGCGATGGGCCGGGTTGCTGACCTGCTGGGCGTCGGTACTGCCGAAACGGTGCGCAAATGGGTCCGCCAGGCTGAGATCGACGCCGGCTCTCGGGCGGGGCAGACCAGCGAGGAATCCGAGGTCCTGCGCAAGCTGCGCCGGGAGAATGCCGAACTCAAGCGGGCCAACGCGATCTTGAAGGCGGCCTCGGTTTTCTTCGCCGCCGAGCTCGACCGGCCCTCTCAGTAGTCGTGGAGTTCATCAGCGCCCACCAGCACATGCGGGTGGGCGTTGATGGTCTCAAGTGGGGTGTCGAGTCGATGTGCGCTGTGCTTTCCGAGTACGGCGTCACGATCGCCCCGTCGACGTATTACGCCCACCGCGCCCGTCAGGGCCCCTCGAAAGCCGACTGGGCCGATGCGCAGGTGATCGATGCGATCTGGCAGCTTCGCCAATCGCAGAGTCTGTACCGAGTCCTGGGCGCTCGTAAGACATGGATTGTATTGCGCACCAATGGTATCGACGTATCGCGCTGTGTCGTGGAACGGGTCATGCGGGAGATGGGTTGGCGGGGTGCGTGCAAGCGCCGGCGGGTGCGCACCACCGTCGCCGATCCGGCAGCGACGCGAGCCCCGGATCGGGTCCGGCGTAATTTCGTCGCCGGTGCGCCTGACCGATTGTGGGTGGCCGATTTCACCTACTGCCGGACTCGTGCCGGCTGGGCCTACACGGCGTTCGTCACCGATGTCTATGCCCGCAAGATCGTGGGTTGGAAGGTGGCTACCGAGATGACCCAGAAGCTGGTGACCGATGCGATCGACCACGCTATCGACACCAGAAAGCGTTCGGGTGCAGCATCTTTGGATTCACTTATCCACCACAGCGACGCGGGCTCGCAATACACCGCGGTCGCGTTCACCGAACGTCTGGCCGCTGAGGGGATCCTGCCTTCAGTCGGATCCGTCGGGGATAGTTTCGATAATGCTCTGGCCGAATCGGTGAACAGCAGCTACAAGACCGAACTCATCGACCGCCAGCCGCTCTATCCCGGTGCCACCGAACTGGCGCTGGGGACCGCCGAATGGGTGGCCTTCTACAACCGTCAGCGACCCAACGGCTACTGCCAGGACCTGACCCCCGATCGGGCCGAAGCGCTCTACTACCATCGCCAACGGCACCCTCATGCCGAGGAGGCACTCAGATAA

Protein Sequence

VEFISAHQHMRVGVDGLKWGVESMCAVLSEYGVTIAPSTYYAHRARQGPSKADWADAQVIDAIWQLRQSQSLYRVLGARKTWIVLRTNGIDVSRCVVERVMREMGWRGACKRRRVRTTVADPAATRAPDRVRRNFVAGAPDRLWVADFTYCRTRAGWAYTAFVTDVYARKIVGWKVATEMTQKLVTDAIDHAIDTRKRSGAASLDSLIHHSDAGSQYTAVAFTERLAAEGILPSVGSVGDSFDNALAESVNSSYKTELIDRQPLYPGATELALGTAEWVAFYNRQRPNGYCQDLTPDRAEALYYHRQRHPHAEEALR_ 
Gene not present in the RLC0011 CRISPRi library.

Specific References

General References