MSMEG2295 (MSMEG2295)

background  +5   0  -5 -10 -15 sgRNA L2FC (+ATc/–ATc) TnSeq Gene Essentiality Predictions (1, 2) Essential Growth Defect Non-Essential Growth Advantage Unknown Operon Legend (3) Operon (1) PMID: 28096490 (2) PMID: 31388080 (3) PMID: 26536359 MSMEG2292 MSMEG2293 dinB MSMEG2295 MSMEG2296 MSMEG2297 MSMEG2299
Species: M. smegmatis
Strain: MC2-155
PATRIC Annotation: -
Synonyms:
M. tuberculosis homologs RVBD3055, RVBD3405c, RVBD2506, RVBD0078, RVBD1019, [+ 2 remaining orthologs]
M. abscessus homologs MAB3418, MAB0476c, MAB2290, MAB4384, MAB3151, [+ 4 remaining orthologs]
Gene Start: 2378620
Gene End: 2379234
Vulnerability Index (VI):
VI Lower Bound:
VI Upper Bound:
[AlphaFold] [Orthologs] [Phyre2] [UniProt] [MycoBrowser] [TnSeq DB]

Essentiality:

Call Condition Type Source Notes
Growth Advantage in vitro TnSeq [Akusobi et al. (2022)]
Unknown in vitro CRISPRi [Bosch & DeJesus et al. (2021)]


DNA and Protein Sequence help

DNA Sequence

GCAGCGCCGCGGCCGGCTCGGGCACATCAGCGGTGTCGATGTCTTCGTTGTAGAACGGCACCTCGGCCAGCCGGTCGAACACCTGCAGGTCCACACCGTCGGGCGCGGTCTCGACTGCCAGTTCGGCGAGTTGGCGGTTGATGGACGCCGCCCGGAGGCTGCCGACGAGTACCAACACCTTGATTTCGGCCATGAGCCGTCCTTTCATGAGATTCTTGTGGTCGGGTTTCGTCCCCGATCAACCGGACCGCAGTCCGATTCATTCCCACTCGGCCGGATTCACAGAAATTCACAGATAAGGTGGCTGTCGTGGCAGCCACTGACCGCTCCGTCCGGCTCGAGGTCACCGACCGCGCGCCGCAGGAGCGCGGCGACGCCGCCCGGAACCGGGCCGCGATCCTCGACGCGGCGCGCCGGCTCATCGCCGAGCGCGGACCCGACGCCGTGAGCACCGACGACATCGCCGTCGAGGCCGGCGTCGGCAAGGGCACCCTGTTCCGCCGCTTCGGCACCCGCGCCGGGCTGATGATGGTGCTGCTCGACGAGGACGAGAAGGCCCACCAGGACGCGTTCATCTTCGGGCCGCCGCCGCTCGGTCCCGGGGCCCCTCCCCTCGACCGTCTGCTCGCCTACGGCCGCGAACGGATGAAGTTCGTCCGCGAGCACCACGCACTGCTCTCCGACGCCGCGCGCGATCCGCAGGCACGCTTCGGCGGGCCGGACACGCTGCACCGGTCCCACGTCCGGATGCTGCTGCAGGCCGCGGGGACCACGGGCGACCTCGACGCACAGGCCGACGCGCTCGTCGCGCTGCTCGACGCCGACTACGTCGCCTACCAACTGGAACGCGGACGCAGCTGCGAGGTCCAGGCCGCCGCCTGGGCCGACACCGCACGTAAGTTGTGTGGCCACTAG

Protein Sequence

VAVVAATDRSVRLEVTDRAPQERGDAARNRAAILDAARRLIAERGPDAVSTDDIAVEAGVGKGTLFRRFGTRAGLMMVLLDEDEKAHQDAFIFGPPPLGPGAPPLDRLLAYGRERMKFVREHHALLSDAARDPQARFGGPDTLHRSHVRMLLQAAGTTGDLDAQADALVALLDADYVAYQLERGRSCEVQAAAWADTARKLCGH_ 
Gene not present in the RLC0011 CRISPRi library.

Specific References

General References