MSMEG3756 (rrlA)

background  +5   0  -5 -10 -15 sgRNA L2FC (+ATc/–ATc) TnSeq Gene Essentiality Predictions (1, 2) Essential Growth Defect Non-Essential Growth Advantage Unknown Operon Legend (3) Operon (1) PMID: 28096490 (2) PMID: 31388080 (3) PMID: 26536359 MSMEG3751 MSMEG3752 MSMEG3753 MSMEG3754 rrfA rrlA rrsA tyrS
Species: M. smegmatis
Strain: MC2-155
PATRIC Annotation: -
Synonyms:
M. tuberculosis homologs
M. abscessus homologs
Gene Start: 3820128
Gene End: 3823247
Vulnerability Index (VI):
VI Lower Bound:
VI Upper Bound:
[AlphaFold] [Orthologs] [Phyre2] [UniProt] [MycoBrowser] [TnSeq DB]

Essentiality:

Call Condition Type Source Notes
Unknown in vitro TnSeq [Akusobi et al. (2022)]
Unknown in vitro CRISPRi [Bosch & DeJesus et al. (2021)]


DNA and Protein Sequence help

DNA Sequence

TGGCGCCGGCTTGGTGCACAGCAAACGTTGAGATGCGGTGTGGGAAACGCTGTTTCGATGGACTGCCAGACACACTATTGGGCCCTGAGACAACAGGCCCGTTGTTCCCTGGCCACTGTGTGTGGTGGGAGGCGTGTTGTTGCCCTGCTTTGGTGGTGGGGTGTGGTGTTTGATTTGTGGATAGTGGTTGCGAGCATCTAGTTCGTAAGAGTGTGGCTGCCGGCCTTTGAGGTTGGGTGGCGCATTGTTGCGGACAAATTGATGTGCAATTTTTCTTCTGATTATTGGTTTTTTGTGTTGTAAGTGTTTAAGGGCGCATGGTGGATGCCTTGGCACTGGGAGCCGATGAAGGACGTAGGAGGCTGCGATAAGCCTCGGGGAGCTGTCAACCGAGCGTTGATCCGAGGATGTCCGAATGGGGAAACCCGGCACGAGTGATGTCGTGTCACCAGGCGCTGAATATATAGGCGTCTGGGGGGAACGCGGGGAAGTGAAACATCTCAGTACCCGTAGGAAGAGAAAACAAAATGTGATTCCGTGAGTAGTGGCGAGCGAAAGCGGAGGATGGCTAAACCGTATGCATGTGATACCGGGTAGGGGTTGTGTGTGCGGGGTTGTGGGACCTATCTTTCCGGCTCTACCTGGCTGGAGGGCAGTGAGAAAATGTTGTGGTTAGCGGAAATGGCTTGGGATGGCCTGCCGTAGACGGTGAGAGCCCGGTACGTGAAAACCCGACGTCTGTCTTGATGGTGTTCCCGAGTAGCAGCGGGCCCGTGGAATCTGCTGTGAATCTGCCGGGACCACCCGGTAAGCCTGAATACTTCCCAGTGACCGATAGCGGATTAGTACCGTGAGGGAATGGTGAAAAGTACCCCGGGAGGGGAGTGAAAGAGTACCTGAAACCGTGCGCTTACAATCCGTCAGAGCCCTCGACGTGTCGTGGGGTGATGGCGTGCCTTTTGAAGAATGAGCCTGCGAGTCAGGGACATGTCGCGAGGTTAACCCGGGTGGGGTAGCCGCAGCGAAAGCGAGTCTGAATAGGGCGTATCCACACAAGAGTGTGTGGTGTAGTGGTGTGTTCTGGACCCGAAGCGGAGTGATCTACCCATGGCCAGGGTGAAGCGCGGGTAAGACCGCGTGGAGGCCCGAACCCACTTAGGTTGAAGACTGAGGGGATGAGCTGTGGGTAGGGGTGAAAGGCCAATCAAACTCCGTGATAGCTGGTTCTCCCCGAAATGCATTTAGGTGCAGCGTCGCATGTTTCTTGCCGGAGGTAGAGCTACTGGATGGCCGATGGGCCCCACAGGGTTACTGACGTCAGCCAAACTCCGAATGCCGGTAAGTCCAAGAGTGCGGCAGTGGGACGGCGGGGGATAAGCTCCGTGCGTCGAGAGGGAAACAGCCCAGATCGCCGGCTAAGGCCCCTAAGCGTGTGCTAAGTGGAAAAGGATGTGCAGTCGCGAAGACAACCAGGAGGTTGGCTTAGAAGCAGCCACCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTGTGCGCCGATAATGTAGCGGGGCTCAAGCACACCGCCGAAGCCGCGGCAGCCAACGTGTTGGCTGGGTAGGGGAGCGTCCTGCATCCGGTGAAGCCGCCGAGTGATCGAGTGGTGGAGGGTGTGGGAGTGAGAATGCAGGCATGAGTAGCGATTAGGCAAGTGAGAACCTTGCCCGCCGAAAGACCAAGGGTTCCTGGGCCAGGCCAGTCCGCCCAGGGTGAGTCGGGACCTAAGGCGAGGCCGACAGGCGTAGTCGATGGACAACGGGTTGATATTCCCGTACCCGTGTATGTGCGTCCATGATGAATCAGCGGTACTAACCATCCAAAACCACCGTGACCGCACCTTTCGGGGTGTGGCGTTGGTGGGGCTGCATGGGACCTTCGTTGGTAGTAGTCAAGCGATGGGGTGACGCAGGAAGGTAGCCGTACCGGTCAGTGGTAATACCGGGGTAAGCCTGTAGGGAGTCAGATAGGTAAATCCGTCTGGCATATATCCTGAGAGGTGATGCATAGCCGAGTGAGGCGAATTCGGTGATCCTATGCTGCCGAGAAAAGCCTCTAGCGAGGACATACACGGCCCGTACCCCAAACCAACACAGGTGGTCAGGTAGAGAATACTAAGGCGTACGAGTGAACTATGGTTAAGGAACTCGGCAAAATGCCCCCGTAACTTCGGGAGAAGGGGGACCCACATGGCGTGTAAGCCTTTACGGCCCAAGCGTGAGTGGGTGGCACAAACCAGTGAGAAGCGACTGTTTACTAAAAACACAGGTCCGTGCGAAGTCGCAAGACGATGTATACGGACTGACGCCTGCCCGGTGCTGGAAGGTTAAGAGGACCCGTTAACTCCCTTTGGGGGTGAAGCGGAGAATTTAAGCCCCAGTAAACGGCGGTGGTAACTATAACCATCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGACTTCTCAACTGTCTCAACCATAGACTCGGCGAAATTGCACTACGAGTAAAGATGCTCGTTACGCGCGGCAGGACGAAAAGACCCCGGGACCTTCACTACAACTTGGTATTGGTGCTCGATACGGTTTGTGTAGGATAGGTGGGAGACTGTGAAGCTCACACGCCAGTGTGGGTGGAGTCGTTGTTGAAATACCACTCTGATCGTATTGGGCCTCTAACCTCGGACCGTATATCCGGTTCAGGGACAGTGCCTGGTGGGTAGTTTAACTGGGGCGGTTGCCTCCTAAAATGTAACGGAGGCGCCCAAAGGTTCCCTCAACCTGGACGGCAATCAGGTGTTGAGTGTAAGTGCACAAGGGAGCTTGACTGCGAGACGGACATGTCGAGCAGGGACGAAAGTCGGGACTAGTGATCCGGCACCTCTGAGTGGAAGGGGTGTCGCTCAACGGATAAAAGGTACCCCGGGGATAACAGGCTGATCTTCCCCAAGAGTCCATATCGACGGGATGGTTTGGCACCTCGATGTCGGCTCGTCGCATCCTGGGGCTGGAGCAGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGCACGCGAGCTGGGTTTAGAACGTCGTGAGACAGTTCGGTCTCTATCCGCCGCGCGCGTCAGAAGCTTGAGGAAACCTGTCCCTAGTACGAGAGGACCGGGACGGACGAACCTCTGGTATACCAGTTGTCCCACCAGGGGCACGGCTGGATAGCCACGTTCGGACAGGATAACCGCTGAAAGCATCTAAGCGGGAAACCTCTTCCAAGACCAGGCTTCTCACCCTCTAGGAGGGATAAGGCCCCCCGCAGACCACGGGATTGATAGACCAGACCTGGAAGCCTAGTAATAGGTGCAGGGAACTGGCACTAACCGGCCGAAAACTTAC

Protein Sequence

_VFKGAWWMPWHWEPMKDVGGCDKPRGAVNRALIRGCPNGETRHE_CRVTRR_IYRRLGGTRGSETSQYP_EEKTKCDSVSSGERKRRMAKPYACDTG_GLCVRGCGTYLSGSTWLEGSEKMLWLAEMAWDGLP_TVRARYVKTRRLS_WCSRVAAGPWNLL_ICRDHPVSLNTSQ_PIAD_YREGMVKSTPGGE_KST_NRALTIRQSPRRVVG_WRAF_RMSLRVRDMSRG_PGWGSRSESESE_GVSTQECVV_WCVLDPKRSDLPMARVKRG_DRVEARTHLG_RLRG_AVGRGERPIKLRDSWFSPKCI_VQRRMFLAGGRATGWPMGPTGLLTSAKLRMPVSPRVRQWDGGG_APCVERETAQIAG_GP_ACAKWKRMCSREDNQEVGLEAATLERVRNSSLVK_LCADNVAGLKHTAEAAAANVLAG_GSVLHPVKPPSDRVVEGVGVRMQA_VAIRQVRTLPAERPRVPGPGQSAQGESGPKARPTGVVDGQRVDIPVPVYVRP__ISGTNHPKPP_PHLSGCGVGGAAWDLRW__SSDGVTQEGSRTGQW_YRGKPVGSQIGKSVWHIS_EVMHSRVRRIR_SYAAEKSL_RGHTRPVPQTNTGGQVENTKAYE_TMVKELGKMPP_LREKGDPHGV_AFTAQA_VGGTNQ_EATVY_KHRSVRSRKTMYTD_RLPGAGRLRGPVNSLWG_SGEFKPQ_TAVVTITILR_RNSLSGKFRPARMA_RLLNCLNHRLGEIALRVKMLVTRGRTKRPRDLHYNLVLVLDTVCVG_VGDCEAHTPVWVESLLKYHSDRIGPLTSDRISGSGTVPGG_FNWGGCLLKCNGGAQRFPQPGRQSGVECKCTRELDCETDMSSRDESRD__SGTSEWKGCRSTDKRYPGDNRLIFPKSPYRRDGLAPRCRLVASWGWSRSQGLGCSPIKAARELGLERRETVRSLSAARVRSLRKPVPSTRGPGRTNLWYTSCPTRGTAG_PRSDRITAESI_AGNLFQDQASHPLGGIRPPADHGIDRPDLEA___VQGTGTNRPKTY 
Gene not present in the RLC0011 CRISPRi library.

Specific References

General References