topA
mL0200
Probable DNA topoisomerase I TopA (omega-protein) (relaxing enzyme) (untwisting enzyme) (swivelase) (type I DNA topoisomerase) (nicking-closing enzyme) (topt I)
M. leprae · TN
[TnSeq DB] [SeqHub]
TN Coordinates: 266033 – 268876
Vulnerability Index (VI):
Click the tabs above to view different types of data for this gene. The Vulnerability tab shows CRISPRi fitness data.
DNA and Protein Sequence help

DNA Sequence 

>mL0200_topA_dna
ATGAAGGGCCCCAGGGGTTCTAGGAGCGGCAGAAATGGGAGCCCGCGGCGACTTGTTATAGTCGAGTCGCCGACGAAAGCGCGCAAATTGTCCGGCTATCTAGGATCTGCGTACATAGTCGAATCATCCCGGGGGCATATCCGTGACCTGCCGCGGACGGCGGCTGATGTGCCCGCGAAGTTCAAGTCTGAGCCGTGGGCTCGGCTGGGCGTAAACGTCGACGCAGACTTTGAACCGCTCTACATCATCACCCCCGAGAAGAAGAGCACCGTCACTGAACTCAAAGGTCTGCTCCAAGGAGTCGACGAGCTCTATCTGGCCACAGATGGTGACCGCGAGGGCGAAGCCATCGCCTGGCACCTGATGCAGACTCTGAAACCGCGAGTTCCGGTCAAGCGGATGGTTTTCCACGAGATCACCGAGCACGCGATCCTCGCCGCCGCTGCGCATCCCCGTGAACTGGATATTGACCTGGTTGACGCGCAGGAGACCCGACGCATCCTGGACCGGCTGTACGGGTATGAAGTTAGTCCGGTACTGTGGAAGAAGGTAGCGCCTAAGCTTTCGGCGGGCCGAGTGCAATCGGTGGCCACCCGCATCATCGTGCAGCGTGAACGCGACCGGATGGCGTTCTGCAGCGCGGTGTACTGGGACATTGTTGCCAAGCTGGACGCCAGCGTGTCCGATCCGACCGCCCAGCCGCTCACCTTTGTCGCCCGTCTGACGGCTGTAGATGGCTTGCGGGTCGCCACCGGCCGCGATTTCGGTGCGATGGGCACGCTGCGTGGAGACTGTGAGGTCTCCAAAATCATTGTCCTCGACGACCTGACCGCAACCACGTTGGCCGCAGGATTGCGCGGCGCTCAGCTTACCGTGGCCTCAGCGGAAGAGAAGCTCTACACTCGGCGACCGTATCCGCCATTCATGACGTCGACGCTGCAACAGGAAGCCGGACGCAAGTTGCGATTCTCTGCGGAACGCACGATGAGCATCGCCCAGCGGCTATATGAGAACGGTTACATTACCTACATGCGTACCGACTCGACCACGTTGTCTGAGTCGGCTATCAACGCCGCTCGTACGCAGGCGCGTCAGCTCTACGGCGAGGAGTACGTCTCTGACTCGCCGCGTCAGTACACCCGCAAGGTAAAGAACGCACAGGAAGCCCACGAGGCCATCCGGCCTGCAGGAGAGACGTTCGCTACCCCGGACGCGGTGTGTAATGAACTCGACGGTGACGAATTCCGGATCTACGAGCTGGTATGGCGGCGTACTGTAGCTTCTCAGATGGCCGACGCTCGCGGTACCACGCTGAGCTTGCGGATCGAGGGCCGAGCCGGCGAGCAACACGTTGTCTTCTCAGCTTCCGGGCGCACCCTGACCTTCCCCGGCTTCCTGAAGGCCTATGTGGAAACCGTGGACGAGCTGGCCGGCGGCGAGGCTGACGACGCTGAGCGTCGACTACCGCATCTGACCTCTGGTCAGCTGCTGGATGTCATCGATCTGACTCCGGACGGGCACGCCACCAACCCTCCGGCCCGCTATACGGAAGCTTCTTTGGTTAAAGCGTTGGAGGAGTTGGGTATCGGCCGCCCGTCGACGTACTCATCGATCATAAAGACCGTCCAGGACCGCGGCTATGTGCAGAAGAAGGGCAGCGCCCTGGTGCCGTCGTGGGTCGCGTTCGCAGTTACCGGCCTTTTGGAGCAGCATTTCGGCCGTCTCGTCGACTACGATTTCACCGCCGCGATGGAAGACGAGCTCGATGAGATCGCTAACGGGAATGAGCAACGTACCAACTGGCTCAACAACTTCTACTTTGGTGGCAACCACGGTGTGTCCGATTCAATAGCCCGCTCCGGCGGCTTGAAGAAGTTGGTCGGCGTGAATATTGAAGGGATTGATGCACGAGAGGTCAATTCCATCAAACTGTTTGACGATGAGCACGGTCGTCCTGTCTATGTTCGGGTAGGCAAGACTGGGCCGTATCTGGAACGCTTGTTGGCAGGCGATGATGGTGAACTTACCCCGCAGCGGGCAAACCTCAACGGCACACTCACTCCGGATGAGCTCACCTTGGAGGTTGCCGAGGAGCTTTTCGCCACACCGCACGAGGGGCGCGTTCTGGGGGTGGATCCCGAAACTGGCCACGAGATCGTCGCCAAAGACGGTCGGTACGGGCCTTATGTGACTGAGGTCCTGCCTAAGCACGATGACGACTATGGTGCAGCGGATCAGGGAACTAAGAAAACTAAGAAGGGTAGGAGGGCTTCCGCTTCTCAAGGCCCCAAACCGAGGACCGGGTCTTTGCTGCGGAGCATGGATTTGCAAACGATCACTCTCGAGGATGCATTGAAACTGTTGTTGCTGCCTCGGGTTGTCGGTGTGGACCCTGCGTCCGGTGAAGAGATCACCGCGCAGAACGGGCGTTATGGTCCATATCTGAAGCGCGGCAAAGATTCTCGACCACTAACAACTGAAGACCAGATGTTCATCATTACTCTCGACGAGGCGTTAAAGATCTACGCTGAACCGAAACGTGCTGGTCGACAAAGTACTTCGCCGCCGCCTTTGCGTGAGCTGGGGACTGATCCGACGTCGGGCAAGCCAATGCTGATCAAGGACGGCCGGTTCGGCCCGTATGTCACCGACGGTGAGACCAACGCTAGCCTGCGCAAGGGTGATGACGTGGTGTCGATAACCGATGAACGTGCCGCCGAATTGTTGGCTGACCGACGGGCCCGAGGTCCGGTTAAGCGGCCTGCTAAGAAGGCTCGCAAGGTCCCGGCGAAGAAGGCAGCTAGACTGGCTCCGGCCCGCGGGATCAGCCAGTCACCTCGCTAG

Protein Sequence 

>ML0200_TopA_protein
MKGPRGSRSGRNGSPRRLVIVESPTKARKLSGYLGSAYIVESSRGHIRDLPRTAADVPAKFKSEPWARLGVNVDADFEPLYIITPEKKSTVTELKGLLQGVDELYLATDGDREGEAIAWHLMQTLKPRVPVKRMVFHEITEHAILAAAAHPRELDIDLVDAQETRRILDRLYGYEVSPVLWKKVAPKLSAGRVQSVATRIIVQRERDRMAFCSAVYWDIVAKLDASVSDPTAQPLTFVARLTAVDGLRVATGRDFGAMGTLRGDCEVSKIIVLDDLTATTLAAGLRGAQLTVASAEEKLYTRRPYPPFMTSTLQQEAGRKLRFSAERTMSIAQRLYENGYITYMRTDSTTLSESAINAARTQARQLYGEEYVSDSPRQYTRKVKNAQEAHEAIRPAGETFATPDAVCNELDGDEFRIYELVWRRTVASQMADARGTTLSLRIEGRAGEQHVVFSASGRTLTFPGFLKAYVETVDELAGGEADDAERRLPHLTSGQLLDVIDLTPDGHATNPPARYTEASLVKALEELGIGRPSTYSSIIKTVQDRGYVQKKGSALVPSWVAFAVTGLLEQHFGRLVDYDFTAAMEDELDEIANGNEQRTNWLNNFYFGGNHGVSDSIARSGGLKKLVGVNIEGIDAREVNSIKLFDDEHGRPVYVRVGKTGPYLERLLAGDDGELTPQRANLNGTLTPDELTLEVAEELFATPHEGRVLGVDPETGHEIVAKDGRYGPYVTEVLPKHDDDYGAADQGTKKTKKGRRASASQGPKPRTGSLLRSMDLQTITLEDALKLLLLPRVVGVDPASGEEITAQNGRYGPYLKRGKDSRPLTTEDQMFIITLDEALKIYAEPKRAGRQSTSPPPLRELGTDPTSGKPMLIKDGRFGPYVTDGETNASLRKGDDVVSITDERAAELLADRRARGPVKRPAKKARKVPAKKAARLAPARGISQSPR

No essentiality data available for this gene.

AlphaFold Protein Interactions

Protein–protein interactions (PPIs) were predicted by co-folding TopA with every other protein in the M. leprae proteome using a pooled-AlphaFold3 based approach (Horia et al.). The size-corrected interface predicted template modeling (sciPTM) score indicates confidence in the predicted interaction: weak evidence (sciPTM > 0.05), intermediate evidence (sciPTM > 0.2), or strong evidence (sciPTM > 0.4).

0 proteins show strong predicted evidence for PPIs

0 show intermediate predicted evidence for PPIs

35 show weak predicted evidence for PPIs

View all pooled AlphaFold results for TopA

Use of these data is subject to the AlphaFold Server Output Terms of Use.

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