dnaE
mL1207
PROBABLE DNA POLYMERASE III (ALPHA CHAIN) DNAE1 (DNA NUCLEOTIDYLTRANSFERASE)
M. leprae · TN
[TnSeq DB] [SeqHub]
TN Coordinates: 1423017 – 1426550
Vulnerability Index (VI):
Click the tabs above to view different types of data for this gene. The Vulnerability tab shows CRISPRi fitness data.
DNA and Protein Sequence help

DNA Sequence 

>mL1207_dnaE_dna
AACCAGTCTTCCTTCGTTCACCTGCATAACCACACCGAGTACTCGATGCTGGACGGTGCTGCGAAGATCACGCCCATGTTCGCCGAGGTCGAACGGCTACAGATGCCCGCGGTTGGCATGACCGATCACGGAAACATGTTCGGTGCGAGCGAGTTTTACAATACGGCCATCAAAGCCGGAATCAAGCCGATTATCGGTGTGGAAGCTTACATCGCTCCTGGATCCCGTTTTGACACCCGGCGCATTACCTGGGGTGACCCCAGCCAGAAGGCTGACGATGTCTCGGCGGGTGGTGCTTACACGCACTTGACGATGGTGGCAGAGAATGCGGCAGGTCTGCGCAACCTGTTCAAGTTGTCTTCGCTGGCTTCGTTCGAGGGGCAGTTGAGCAAGTGGTCGCGAATGGATGCCGAGCTTATCGGTGAATATGCCGAGGGTATCATTGTAACTACTGGCTGCCCCTCCGGTGAGGTGCAGACCCGGCTTCGCCTCGGGCACGACCGGGAGGCGTTGGAATCGGCCGCCAAGTGGCGCGAGATCGTTGGCCCCGATAACTACTTTCTCGAGCTGATGGACCACGGCTTGTCTATCGAGCAACGTGTCCGCGAAGGACTGCTTAATATCGGCCGCAAGCTCAACATTCCTCCACTGGCCACCAACGACTGCCACTATGTCACCCGCGACGCTGTGCACAACCACGAGGCGTTGTTGTGTGTACAGACCGGCAAGACCTTGTCGGATCCGAACCGGTTCAAGTTCCACGGTGACGGCTATTATCTCAAGTCGGCTGCCGAGATGCGCCAGCTTTGGGACGACGAAGTTCCTGGGGCCTGCGACTCCACGCTGCTGATCGCCGAGCGGGTGCAGTCCTACGCCGATGTGTGGGAACCGCGCAATCGGATGCCTGTCTTCCCTGTTCCCGTGGGACACGACCAGGCGTCTTGGCTACGTCACGAGGTGGATGCCGGCCTCAAGCGCCGATTCCCCGATGGCCCACCCAACGGATATGTCGAACGGGCGGCCTACGAGATCGACGTCATTTGTGACAAGGGTTTTCCTGCCTACTTTCTGATCGTTGCAGACCTGGTCAACCATGCCCGGTCTGTCGGGATCCGCGTGGGCCCGGGCCGCGGTTCGGCAGCCGGGTCGCTTGCCGCCTATGCCCTGGGGATCACTGACATCGACCCGATACCGCACGGTCTGCTGTTCGAGCGGTTTCTCAACCCGGAACGCACCTCGATGCCTGACATTGATATCGACTTCGACGACCGTCGCCGCGGTGAGATGGTGCGCTACGCCGCCGACAAGTGGGGGCATGACAGAGTCGCTCAGGTCATCACTTTCGGTACCATTAAAACCAAAGCGGCGCTGAAGGATTCTGCGCGGATCCACTACGGGCAACCTGGTTTTGCTATGGCCGACCGTATCACCAAGGCATTACCGCCGGCCATAATGGCCAAAGACATCCCGTTGTCGGGAATCACCGATCCGGCCCACGAACGGTTCAAGGAAGCCGCCGAGGTCCGCAGCCTGATCGAAACTGATTCGGACGTGCGCATCATCTACCAGACTGCTCGCGGGCTGGAGGGTCTGATCCGCAACGCCGGCGTGCACGCATGCGCGGTGATCCTGAGTAGTGAGCCGTTGACCGAGGCGATCCCGCTGTGGAAGCGACCGCAGGACGGGGCCATCATCACCGGCTGGGACTACCCAGCATGCGAGGCCATCGGTCTGTTGAAGATGGACTTCCTGGGTCTACGTAACCTGACGATCATCGGCGATGCGATCGAAAACATTAAGACCAACAGGGGAATTGATCTCGATCTGGAATCCGTGCCACTGGACGACCAGGCCACCTACGAGCTGCTTGGCCGCGGTGACACCCTGGGTGTGTTTCAGCTCGACGGTGGGCCCATGCGCGACCTACTGCGTCGCATGCAACCGACCGGATTCGAAGACATCGTCGCGGTACTAGCGTTGTATCGGCCCGGACCGATGGGCATGAACGCGCACAACGACTACGCCGACCGCAAGAACAACCGGCAGGTAATCAAACCCATTCATCCCGAACTCGAGGAACCGCTGCGTGAGATCCTCGCTGAAACCTATGGACTCATCGTCTACCAAGAGCAGATCATGCGAATCGCGCAGAAGGTGGCCGGTTACTCTTTGGCCCGAGCGGACATTCTGCGTAAGGCGATGGGAAAAAAGAAGCGCGAGGTGCTGGAGAAGGAGTTCGAGGGTTTTTCGGAAGGTATGCAGGCCAACGGATTCTCGGTCCACGCCATCAAGGCGTTGTGGGACATCATCTTACCTTTCGCCGACTACGCATTCAACAAGTCTCACGCCGCTGGCTATGGCCTAATATCCTATTGGACCGCCTATCTCAAGGCCAACTTTGCTGGTGAATACATGGCGGGCCTGTTGACTTCGGTCGGCGACGATAAAGACAAGGCAGCTGTCTATCTGGCCGACTGCCGCAAGTTTGGCATCACGGTGCTGCCGCCGGACGTCAACGAGTCCGTCCTGGACTTCGCCTCGGTGGGTGCTGACATCCGCTACGGGTTGGGCGCGGTGCGCAATGTCGGTGCCAACGTGGTAGGTTCGTTGATCAAGACTCGCAATGCCAAGGGCAAATTTGCTGACTTCTCGGACTATCTGAACAAGATCGACATTACGTCCTGCAACAAGAAAGTCACTGAATCGCTGATCAAGGCAGGAGCCTTCGATTCGTTAGGGCATTCTCGCAAAGGTCTTTTCCTGGTCCACGCTGACGCTGTGGATTCGGTGCTAGGCACCAAGAAAGCGGAAGCGATCGGGCAGTTCGATCTGTTCGGCGGAACTGATGGCGGCACTGACGCGGTATTTACCATCAAAGTTCCTGACGACGAATGGGAGGATAAACACAAGCTGGCTCTGGAACGGGAGATGCTGGGCTTGTATGTGTCCGGGCATCCGCTCAACGGAGTGGCACACCTGTTGGCTGCTCAGGTAGACACCGCCATTCCGACGATTCTCGACGGTGGTGTCTCCAATGATACTCAGGTGCGGGTGGGTGGCATTCTGGCCGCGGTGAACCGGCGCGTCAACAAAAACGGAATTCCTTGGGCGTCAGCGCAATTAGATGATTTGACTGGAGGGATCGAGGTGATGTTCTTCCCGCACACATATTCCAGCTACGGCGCCGACATCATCGATGATGCTGTTGTGTTGGTTAATGCCAAGGTGGTGGTTCGTGATGACCGGATTGCGCTGATCGCTAATCAACTTGTTGTTCCTGACTTTTCCAACGTGCAAGAGGACCGGCCGCTGGCGGTTAGTTTGCTGACTCGGCAGTGCACGTTCGACAAGGTAAATGCACTCAAGCAAGTGCTGGCGCGCCACCCCGGCACATCGCAAGTCCATTTGCGGCTTATCAGCGGGGACCGAATCACTACGCTTGAACTAGACCAGTCGCTGCGGGTGACATCGTCGCCGGCGTTGATGGGAGACCTTAAGGCGCTGCTCGGTCCCGGGTGTTTGGGTGATTAGCAA

Protein Sequence 

>ML1207_DnaE_protein
NQSSFVHLHNHTEYSMLDGAAKITPMFAEVERLQMPAVGMTDHGNMFGASEFYNTAIKAGIKPIIGVEAYIAPGSRFDTRRITWGDPSQKADDVSAGGAYTHLTMVAENAAGLRNLFKLSSLASFEGQLSKWSRMDAELIGEYAEGIIVTTGCPSGEVQTRLRLGHDREALESAAKWREIVGPDNYFLELMDHGLSIEQRVREGLLNIGRKLNIPPLATNDCHYVTRDAVHNHEALLCVQTGKTLSDPNRFKFHGDGYYLKSAAEMRQLWDDEVPGACDSTLLIAERVQSYADVWEPRNRMPVFPVPVGHDQASWLRHEVDAGLKRRFPDGPPNGYVERAAYEIDVICDKGFPAYFLIVADLVNHARSVGIRVGPGRGSAAGSLAAYALGITDIDPIPHGLLFERFLNPERTSMPDIDIDFDDRRRGEMVRYAADKWGHDRVAQVITFGTIKTKAALKDSARIHYGQPGFAMADRITKALPPAIMAKDIPLSGITDPAHERFKEAAEVRSLIETDSDVRIIYQTARGLEGLIRNAGVHACAVILSSEPLTEAIPLWKRPQDGAIITGWDYPACEAIGLLKMDFLGLRNLTIIGDAIENIKTNRGIDLDLESVPLDDQATYELLGRGDTLGVFQLDGGPMRDLLRRMQPTGFEDIVAVLALYRPGPMGMNAHNDYADRKNNRQVIKPIHPELEEPLREILAETYGLIVYQEQIMRIAQKVAGYSLARADILRKAMGKKKREVLEKEFEGFSEGMQANGFSVHAIKALWDIILPFADYAFNKSHAAGYGLISYWTAYLKANFAGEYMAGLLTSVGDDKDKAAVYLADCRKFGITVLPPDVNESVLDFASVGADIRYGLGAVRNVGANVVGSLIKTRNAKGKFADFSDYLNKIDITSCNKKVTESLIKAGAFDSLGHSRKGLFLVHADAVDSVLGTKKAEAIGQFDLFGGTDGGTDAVFTIKVPDDEWEDKHKLALEREMLGLYVSGHPLNGVAHLLAAQVDTAIPTILDGGVSNDTQVRVGGILAAVNRRVNKNGIPWASAQLDDLTGGIEVMFFPHTYSSYGADIIDDAVVLVNAKVVVRDDRIALIANQLVVPDFSNVQEDRPLAVSLLTRQCTFDKVNALKQVLARHPGTSQVHLRLISGDRITTLELDQSLRVTSSPALMGDLKALLGPGCLGD_Q

No essentiality data available for this gene.

AlphaFold Protein Interactions

Protein–protein interactions (PPIs) were predicted by co-folding DnaE with every other protein in the M. leprae proteome using a pooled-AlphaFold3 based approach (Horia et al.). The size-corrected interface predicted template modeling (sciPTM) score indicates confidence in the predicted interaction: weak evidence (sciPTM > 0.05), intermediate evidence (sciPTM > 0.2), or strong evidence (sciPTM > 0.4).

1 protein shows strong predicted evidence for PPIs

5 show intermediate predicted evidence for PPIs

112 show weak predicted evidence for PPIs

View all pooled AlphaFold results for DnaE

Use of these data is subject to the AlphaFold Server Output Terms of Use.

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