pks4
mL1229
PROBABLE POLYKETIDE BETA-KETOACYL SYNTHASE PKS4
M. leprae · TN
[TnSeq DB] [SeqHub]
TN Coordinates: 1454302 – 1460658
Vulnerability Index (VI):
Click the tabs above to view different types of data for this gene. The Vulnerability tab shows CRISPRi fitness data.
DNA and Protein Sequence help

DNA Sequence 

>mL1229_pks4_dna
CGAGCCGCAAACGTTACGCCGGTGGCTGTCATTGGTATGGCTTGTCGGCTTCCCGGCGGGATCGATTCTCCGCAGCGCCTTTGGGAGGCGTTGTTGCGTGGCGACGACCTGGTGACCGAGATTCCCAAGGAGCGCTGGGACGCGGATTTGTATTACGATCCTGAACCAGGGGTGCCTGGCCGGTCGGTATCACGATGGGGCGCCTTCCTGGATGATGTTGGCGGCTTTGACTGCGATTTCTTTGGTATGACTGAGCGTGAGGCCATCGCGATCGACCCGCAACATCGCCTGTTGCTGGAAACGTCGTGGGATGCCATCGAGCATGCGGGTCTTGACCCAGCGACACTCGCGGGGTCTGCGACCGGCGTCTTTGTGGGACTGACGCATGGGGACTATGAACTGTTGTCCGCCGATTGTGGTGCCACGGAGGGACCGTACGGTTTCACCGGCACCAGTAACAGCTTCGCGTCCGGGCGAGTGGCTTACACACTTGGGTTGCACGGTCCGGCGGTCACGGTGGACACGGCGTGCTCGTCGGGCTTGATGGCTGTGCACCAGGCCCGCGGCAGTTTGGATTGCGGCGAAAGCGATCTGGCCCTTGCCGGTGGCGTCGTCGTGACGCTGGAGCCACGGAAGTCGGTTTCGGGTTCGCTGCAGGGAATGTTGTCGCCCACCGGTCGTTGCCATGCCTTCGATGTCGCAGCCGACGGATTCGTGTCTGGCGAGGGCTGTGTGGTGCTGTTACTCAAGCGGATGCCCGACGCTGAGCGTGACGGTGACCGAATCCTGGCGGTGCTACGCGGCACCGCGGCTAATCAGGATGGCCACACTGTGCACATAGCAGCACCATCGGAGCAGGCACAAATCGCAGTATATCACAAGGCGTTGGCCGTGGGCGGCGTTGACCCCACCACGGTCGGCCTGATCGAAGCGCACGGTACCGGCACCCCGGTCGGAGATCCGATCGAATATGCGAGCCTGGCGGCGGTTTACGGAACTGCGGGACGTTGTGCGCTGGCATCGACTAAGACAAACTTTGGCCACATGCAGTCGACATCAGGACCTCTGGGGATGATGAAGGCAATCCTGGCGCTACAGCATGGCGTGGTTCCGAAAAACCTGCACTTCACTCGGCTGCCTGATCAACTGGCTGAGATTACCACCGAACTTTTTGTGCCGCAGGTTAATACGCCCTGGCCCGTCGATGTCCAGCAGCCACGGCGTGCCGCGGTGTCGTCGTATGGCATGTCTGGCACCAACGTGCACGCGATCCTCGAGCAAGCACCAGAGAATTCAGGTAAGGACTCCCCGGCAGTATCTAAAGTCAATGGCCCGCTGTTGTTTCCGCTGTCGGCCACGTCGGTTGAGCAATTGCGTGCGACGGCTGCCCGAATGGCGGATTGGGTCGACGAACACGGCAGCGGGACTAAACACACTGGGCTGTGGGATTTGGGCTATACGCTGTCGCGCCGCCGTGGATACCGTCCAGTGCGGACGATGGTCCTGGCGAGCAGCTTTGCTGAACTCAGCCGGGGACTGCGTGAAATCGCCGGCAGCGAGATTCCGTATCAGTCCGCTGTGGGGCAAGATGACCGCGGACCGGTGTGGGTGTTCTCCGGGCAGGGGTCACAGTGGGCCGGTATGGGCGCTGAGTTGTTCGCGAAGGAGCCGGTGTTCGCCGCGGCCATCGCGGCGATGGAACCGGTGATTGTCGAGGAATCAGGGTTTTCGGTCACCGAGGCGCTGACGGTGGCTGAGAAGGTAACCGGTATTGACCGGGTCCAGCCGACGATCTTCGCAATCCAGGTGGCGTTGGCTGCCACGATGAAGTCCTACGGAGTGTATCCGGGGGCAGTCATCGGACATTCGCTCGGTGAGGCTGCGGCAGCCGTCGCCGCGGGCGCACTGTCTTGCGAAGACGGTTTGCGCGTCATTTGCCGTCGCTCGCAACTGATGTCGCGTGTTGCGGGCAGCGGCGCTATGGCGTCAGTGGAGCTACCTGGCCAACAAGTGCTTTCCGAACTCTCTATTCGCGGGCTGTCGGATGTTGTGCTGGCCGTCGTAGCCTCACCAACATCGACCGTCGTCGGTGGTGACACCCAGGCGATTCGGCATCTGTTGGCCGCATGGCAGGCACAGGACGTGATGGTGCGTGAGGTAGCTGTCGACGTCGCTTCACATACGCCGCAGGTCGAACCGATTCTTGACGACTTGGTCGAAACCCTTGCCGATCTCAAACCAACTGAGCCGAAAGTTCCGTATTATTCCGCGACCCTGTGGGACCCTCGTGAGCGGCCGTCGTTCACCGGTGAATACTGGGCGGAAAACCTACGCTACACGGTGCGATTCGCGGCGGCCGTGCAGGCGGCACTCAAGGATGGCTTTCGAGTCTTCGGGGAGCTGGCGCCACACCCCCTGTTGACCTATGCGATCGAGCAAAACGCTGCCAGTCTCGACATGCCGGTCAGCGCGTTGGCCGCCATGCGGCGTGAGCAGGAACTGCCGTTCGGATTACGTGGTTTCATCGCCGATGTGCATAGCGCCGGCGCCTTGGTCGATTTCTCCGTCCAGTACCCCAATGGGTGCCTGGTGGATGCACCATTACCTACCTGGACCCGTCGGGAGCTGATGCTGAGTCGCGAGTCGGTGGACCATCCTGCGCACGGCGCCGCGGTCCAGGCTGTTCATCCACTACTCGGGGCACATGTGCATCTGTTCGAGGAACCGGAGCGCCATGTATGGCAAGGCCGGGTCGGCACCGAGGCACATCCATGGCTCGGCGACCACCAGATCCACGACGTAGCCGCGTTTCCCGGTGCTGCTTACTGTGAGATGGCCTTAACCGCCGCGCGCGTGACTCTTGGCGAAGCATCAGAGGTCCGCGACATTAAGTTCGAGCAGACGCTGCTTTTGGATCGTGAAACGATGGCATCGTCGGCGGCAACGGTTTCGGCTCCTGGCGTCCTCGAGTTCGCGGTGGAAACTTATGAGGAAGGCGAACGTGTTCGGCGGGCAAGTGCGGTCTTACGGGCCCTGGAGGACCGAGTCAATCAGCAGCCGGCCTCACACAGCATCGACGCGCTAATCGCCAGCCATCCGCTGCGCACGGAAGGCGCCGATTTGCGGGCAACCTTCGACGCCATCGGCATCCAGTACGGCCTGGCCTTCTCGGGCCTCACTGCAGTATACACTGCCGCTGGGACCAATGAGGATGTCTCTACGGTGCTTGCTGAGGTCGCGCTGCCTGGGGCCATTCGCTCGCAGCAAGCATCATACACAAGTCACCCTGCATTGCTCGATGCGTGCTTCCAGTCGGTCATCGTTGCACCCGAGGTGCAAAAGGCGGGCGCCGGCGGTCTGCTGCTGCCCGTATATGTGCGCAGACTGCTTAACTACCACTCGATGCGCAACGCGCAATACTGCCTCACCCGGGTCAAGACATCGCGAGCTGGTGAGTGCGAAGTTGACTTGGAAGTTCTGGACCAGTCTGGAGCGGTGTTGTTGCTGGTCGAGGGGCTGCGTCTGGCTGGGGGCGCCTCGGAGCACGAATGTGCCAATCGAGTGCTCGACGAGCGGCTGTTTACCGTTGAGTGGGAACCGCGCGAACTGCCTGAGGTGACGCAGCGTGAGCGTGGATCCTGGTTAGTGCTCGGCGCGTCCGCCGAGGATCCGTTGGTTCAGCAACTCGGCGATGCACTAAACGCCGATGGCGGGCATTGCACCGCGGTGTCGTTGCCGCTCGGACAAATCGATACCGCGCAGCTGTGTTCGCTGCTGTCTAGAGACGGCGTGAGTTCCTCCATTGGTCACGGTGCCCTCAAGGGACTGACCGGCGTCGTCGTAGTGACCGCCCCACCCGCCACTGTGGCAGAATCGCCAGCATTGCGGCGCGGCAGGGACTATGTATCTCACCTTATCACTATCGTCCGCGAGATCTCCGAACTCCCCGGTGAGCGGCCTCGCCTCTTTGTAGTGACTAGAAATGCCGCAAGCATCAAGACGGGTGACCTGGCCAACCTGGAGCAGGTCGGGCTGCGCGGGCTGATACGGGTGATTGACTCCGAACACCCGTACTTGAGTGTCACTCAGATCGACGTTGAAGAAACCAACCAAGCGACGATCGCCACACAAGTGGCTCGGCAACTGCGCAGCGGCTCGGATGAGGACGAGACCGGCTGGCGGGACGGCGAGTGGTATACAGCCCGACTGCGCCCCGGTCCGTTGCGCCAGGCCGAACGGCGAACCGTTGTCGTGCAGAACGAACACGATGGCATGCGTCTAGAGATCCGTACTCCGGGTGACCTGGATTCGTTGGAATTCGTTGCTTTCGATCGGGTACCGCCGGGACCGGGGGAGATCGAGGTCGCGGTCACCGCATCCACCGTTAACTTCGCCGACGTTCTGGTCGCGTTCGGTCGGTATCCCACTTTCGAGGGTTACTGCCAACTGTTGGGTATCGACTTCGCTGGAGTGGTGTCGGCAGTCGGGTTAAATGTCACCGATCACAAGGTCGGTGACCGGGTCGGCGGTGTGTCCCGCAACGGTTGCTGGACCACATTTGTGATTTGCGACGCCCGCCACGCGGTCACGCTCTCGCCCGAGGTGCCGCTGGAAGAGGCCGCGGCTGTTCCTACCGTCGCTGCGACCGCTTGGTACGGTCTGCACGATCTGGCCCGGATCTCACAGACCGATAAAGTGCTGATTCATTCGGGGACCGGTGGTGTCGGACAGGCGGCGATCGCGATCGCACGTGCCGCGGGGTGTGAGATCTTCGCGACGGCGGGTAGTCCGCAGCGGCGACAACTGCTGCGCGACAAGGGTATTGAGCATGTCTACGACTCGCGCAGCCTCGAGTTCGCCGAACAGATCCATCGTGACACCAAGGGGTACGGTGTTGACGTCGTGCTCAACTCACTGCCCGGCGCTGCGCAACGCGCCGGAATCGAATTGTTGGCTTTCGGTGGGCGGTTTGTCGAGATCGGCAAACGCGACATCTACGGCGACACCCGACTTGGCTTGTTCCCATTCCGCCGCAACCTTTCGCTGTACGCGGTCGACTTGGCGTTGTTGACTTATACTCACCCAGACACCGTTCGGCGCTTGCTGACCACCGTGTACCAACGCACCGCCGAGGGTGTGCTGCCTGTGCCGCAGACCACGCACTATCCAATTCATAACACGGCTAATGCCGTCCGCCTAGTCGCTGGTGCCGGGCACACCGGCAAGGTGTTGCTGGATGTGCCGCATACCGGATCCAGTGTGGCTGTGGTTCCCCCGGAGCAGGTTCGTACGTTTCGCGCTGACGGTGCCTATATCGTTACTGGCGGGCTGACCGGCTTAGGGCTGTTCCTCGGCACCGAGATGGCTGCAGCGGGCTGCGGACGGATCGTACTCAACTCTCGCTCGGCGCCTAATCCCCAAGCGCAAGAGACTATCGAACGGTTGCGTGCCGGGGGCGCCGATATCCAAGTGGAGTGTGGTGATATCGCTGAGCCTAGGGTCGCTGACCGCCTGGTGGTGGCGGCGACTCAATCGGGGCTTCCGGTTCGTGGCGTGCTACACGCAGCGGGGGTGGTCGAGGACGCTACGTTGACCAACGTCACCGATGACCTCATCGACCGTTGTTGGGCGCCTAAGGTCTACGGCGCGTGGAACCTACACCAGGCCCTGCAAGAAGGAAAAACCGCGCAGCTGCTGGATTGGTTCTGTTCGTTCTCAACGGCCGCCGCGCTGGTCGGTTCGCCGGGTCAGGGCGCATATGCGGCGGCCAACAGTTGGTTGGATGGTTTCTCGTATTGGCGCCGGGCACAGGGCCTTGCCAGCACCACGATCGCTTGGGGGGCTTGGGGTGAGCTTGGTCTTGCCACCGCTTTGGCAGAAATGGCCGGTGTAGCGATCGCACCCACTGAGGGTGCTCGGGTATTTCAGACGCTGCTGCGCTATAATCGTCCTTATTCCTGCTATGCGCCGATCATGGAAACGCCGTGGTTGAAGTCCTTTGCGCAACACAGTCGCTTCGCCGAGGCGTTCCGATCCATTTCCCAAGGCCAAAAAGACAAAAGCAAGTTCCTGGCTGAATTGAAAGTGTTGCCTCGAGACGAATGGCCTAGCATGATTCGACGGCTGGTATCCGAGCAGATCAGCTTGTTACTGCGGCGCACTATTGATCCCGATCGACTGCTATCTGACTATGGTTTGGATTCGCTCGGGAATTTGGAATTACGAACCCGCATCGAGACAGAGACAGGGATACGCATAAGTCCCACTAAAATCACCACAATTCGAAGTCTAGCCGAGCATTTGTGCGAAGAGCTGGCAGCTACGGAAGCTGAATCGGCAGTCACTGCCACTTGATGG

Protein Sequence 

>ML1229_Pks4_protein
RAANVTPVAVIGMACRLPGGIDSPQRLWEALLRGDDLVTEIPKERWDADLYYDPEPGVPGRSVSRWGAFLDDVGGFDCDFFGMTEREAIAIDPQHRLLLETSWDAIEHAGLDPATLAGSATGVFVGLTHGDYELLSADCGATEGPYGFTGTSNSFASGRVAYTLGLHGPAVTVDTACSSGLMAVHQARGSLDCGESDLALAGGVVVTLEPRKSVSGSLQGMLSPTGRCHAFDVAADGFVSGEGCVVLLLKRMPDAERDGDRILAVLRGTAANQDGHTVHIAAPSEQAQIAVYHKALAVGGVDPTTVGLIEAHGTGTPVGDPIEYASLAAVYGTAGRCALASTKTNFGHMQSTSGPLGMMKAILALQHGVVPKNLHFTRLPDQLAEITTELFVPQVNTPWPVDVQQPRRAAVSSYGMSGTNVHAILEQAPENSGKDSPAVSKVNGPLLFPLSATSVEQLRATAARMADWVDEHGSGTKHTGLWDLGYTLSRRRGYRPVRTMVLASSFAELSRGLREIAGSEIPYQSAVGQDDRGPVWVFSGQGSQWAGMGAELFAKEPVFAAAIAAMEPVIVEESGFSVTEALTVAEKVTGIDRVQPTIFAIQVALAATMKSYGVYPGAVIGHSLGEAAAAVAAGALSCEDGLRVICRRSQLMSRVAGSGAMASVELPGQQVLSELSIRGLSDVVLAVVASPTSTVVGGDTQAIRHLLAAWQAQDVMVREVAVDVASHTPQVEPILDDLVETLADLKPTEPKVPYYSATLWDPRERPSFTGEYWAENLRYTVRFAAAVQAALKDGFRVFGELAPHPLLTYAIEQNAASLDMPVSALAAMRREQELPFGLRGFIADVHSAGALVDFSVQYPNGCLVDAPLPTWTRRELMLSRESVDHPAHGAAVQAVHPLLGAHVHLFEEPERHVWQGRVGTEAHPWLGDHQIHDVAAFPGAAYCEMALTAARVTLGEASEVRDIKFEQTLLLDRETMASSAATVSAPGVLEFAVETYEEGERVRRASAVLRALEDRVNQQPASHSIDALIASHPLRTEGADLRATFDAIGIQYGLAFSGLTAVYTAAGTNEDVSTVLAEVALPGAIRSQQASYTSHPALLDACFQSVIVAPEVQKAGAGGLLLPVYVRRLLNYHSMRNAQYCLTRVKTSRAGECEVDLEVLDQSGAVLLLVEGLRLAGGASEHECANRVLDERLFTVEWEPRELPEVTQRERGSWLVLGASAEDPLVQQLGDALNADGGHCTAVSLPLGQIDTAQLCSLLSRDGVSSSIGHGALKGLTGVVVVTAPPATVAESPALRRGRDYVSHLITIVREISELPGERPRLFVVTRNAASIKTGDLANLEQVGLRGLIRVIDSEHPYLSVTQIDVEETNQATIATQVARQLRSGSDEDETGWRDGEWYTARLRPGPLRQAERRTVVVQNEHDGMRLEIRTPGDLDSLEFVAFDRVPPGPGEIEVAVTASTVNFADVLVAFGRYPTFEGYCQLLGIDFAGVVSAVGLNVTDHKVGDRVGGVSRNGCWTTFVICDARHAVTLSPEVPLEEAAAVPTVAATAWYGLHDLARISQTDKVLIHSGTGGVGQAAIAIARAAGCEIFATAGSPQRRQLLRDKGIEHVYDSRSLEFAEQIHRDTKGYGVDVVLNSLPGAAQRAGIELLAFGGRFVEIGKRDIYGDTRLGLFPFRRNLSLYAVDLALLTYTHPDTVRRLLTTVYQRTAEGVLPVPQTTHYPIHNTANAVRLVAGAGHTGKVLLDVPHTGSSVAVVPPEQVRTFRADGAYIVTGGLTGLGLFLGTEMAAAGCGRIVLNSRSAPNPQAQETIERLRAGGADIQVECGDIAEPRVADRLVVAATQSGLPVRGVLHAAGVVEDATLTNVTDDLIDRCWAPKVYGAWNLHQALQEGKTAQLLDWFCSFSTAAALVGSPGQGAYAAANSWLDGFSYWRRAQGLASTTIAWGAWGELGLATALAEMAGVAIAPTEGARVFQTLLRYNRPYSCYAPIMETPWLKSFAQHSRFAEAFRSISQGQKDKSKFLAELKVLPRDEWPSMIRRLVSEQISLLLRRTIDPDRLLSDYGLDSLGNLELRTRIETETGIRISPTKITTIRSLAEHLCEELAATEAESAVTAT_W

No essentiality data available for this gene.

AlphaFold Protein Interactions

Protein–protein interactions (PPIs) were predicted by co-folding Pks4 with every other protein in the M. leprae proteome using a pooled-AlphaFold3 based approach (Horia et al.). The size-corrected interface predicted template modeling (sciPTM) score indicates confidence in the predicted interaction: weak evidence (sciPTM > 0.05), intermediate evidence (sciPTM > 0.2), or strong evidence (sciPTM > 0.4).

2 proteins show strong predicted evidence for PPIs

12 show intermediate predicted evidence for PPIs

21 show weak predicted evidence for PPIs

View all pooled AlphaFold results for Pks4

Use of these data is subject to the AlphaFold Server Output Terms of Use.

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