MSMEG2299 (MSMEG2299)

background  +5   0  -5 -10 -15 sgRNA L2FC (+ATc/–ATc) TnSeq Gene Essentiality Predictions (1, 2) Essential Growth Defect Non-Essential Growth Advantage Unknown Operon Legend (3) Operon (1) PMID: 28096490 (2) PMID: 31388080 (3) PMID: 26536359 dinB MSMEG2295 MSMEG2296 MSMEG2297 MSMEG2299 MSMEG2301 MSMEG2302 MSMEG2303
Species: M. smegmatis
Strain: MC2-155
PATRIC Annotation: -
Synonyms:
M. tuberculosis homologs nrdE
M. abscessus homologs MAB3413c
Gene Start: 2381113
Gene End: 2383233
Vulnerability Index (VI):
VI Lower Bound:
VI Upper Bound:
[AlphaFold] [Orthologs] [Phyre2] [UniProt] [MycoBrowser] [TnSeq DB]

Essentiality:

Call Condition Type Source Notes
Growth Advantage in vitro TnSeq [Akusobi et al. (2022)]
Unknown in vitro CRISPRi [Bosch & DeJesus et al. (2021)]


DNA and Protein Sequence help

DNA Sequence

CCGGACATCAACGCTGGGGGCTACGTCCCCAAGCAGGTCATCGCGTTCCTCAACAACGAACACAACCGGTCGTTGATCCGCGGCGTCATCGCCGCGGGCAACAACAACTTCGGTGCGGAGTTCGCCTATGCGGGCAATGTGATCGCGCGCAAGTGTGGCGTGCCGTACCTGTATCGCTTCGAACTGATGGGTACCCCGGACGATGTGGAAGCCGTCCGGGCAGGTTTGAAAGACTTCTGGAAGGAACAGACGTGCCACCCACCGTCACAGCTGCAGAGCCTGTAACCACCACCGGGCACGTGTTGCCGGGGGAGGCGGATTACCACGCGCTCAACGCGATGCTGAATCTGTACGACGCCGACGGCAAGATTCAGTTCGAAAAGGACAGGGAAGCCGCCAAGCAGTACTTCCTGCAGCACGTCAACCAGAACACGGTCTTCTTCCACAGCCAGGACGAGAAGCTCGACTACCTGATCGAGAACGAGTACTACGAGCGCGAGGTGCTCGACCAGTACTCCCGCGACTTCATCAAGAGCCTGCTGGACCGCGCGTACGCCAAGAAGTTTCGGTTCCCCACGTTCCTCGGTGCGTTCAAGTACTACACGTCCTACACGCTGAAGACCTTCGACGGTAAGCGCTACCTGGAGCGTTTCGAGGACCGCGTCGTCATGGTCGCGTTGACCCTGGCCGCAGGCGACACCGAACTCGCCGAGAAACTCGTCGACGAGATCATCGACGGCCGGTTCCAGCCGGCCACCCCCACGTTCCTCAACTCGGGCAAGAAGCAGCGCGGCGAGCCGGTGTCCTGCTTCCTGTTGCGCATCGAGGACAACATGGAGTCCATCGGGCGTTCCATCAACTCGGCGCTGCAGCTGTCCAAGCGCGGCGGCGGAGTCGCGCTGCTGCTGAGCAACATCCGCGAGCACGGCGCGCCCATCAAGAACATCGAGAACCAGAGCTCGGGCGTCATCCCCATCATGAAGCTGCTGGAGGACTCGTTCTCCTACGCCAATCAGCTGGGTGCCCGTCAGGGCGCGGGCGCGGTGTACCTGCACGCCCACCACCCCGACATCTACCGGTTCCTGGACACCAAGCGCGAGAACGCCGACGAGAAGATCCGGATCAAGACGCTCTCGCTGGGTGTCGTGATCCCCGACATCACCTTCGAGCTGGCCAAGAAGAACGAGGACATGTACCTGTTCTCGCCGTACGACGTCGAGCGCGTCTACGGGGTGCCGTTCGCCGACGTCTCGGTCACCGAGAAGTACTACGAGATGGTCGACGACGCGCGGATCCGCAAGACCAAGATCAACGCGCGCGAGTTCTTCCAGACGCTGGCCGAGCTGCAGTTCGAATCGGGCTACCCGTACATCATGTTCGAGGACACCGTGAACCGGTCCAACCCGATCGCGGGCAAGATCACGCACTCGAACCTGTGCTCGGAGATCCTGCAGGTCTCCACGCCGTCGGAGTTCAACGACGACCTGTCCTACGCCAAGGTGGGCAAGGACATCTCCTGCAACCTCGGCTCGCTCAACATCGCCAAGGCCATGGACTCGCCGGACTTCGCGCAGACCATCGAGGTGGCGATCCGTGCGCTCACCGCGGTGAGCGATCAGACCCACATCACGTCCGTGCCGTCGATCGAGCAGGGCAACAACGATTCCCACGCCATCGGCCTGGGGCAGATGAACCTGCACGGATACCTTGCGCGGGAACGCATCTACTACGGTTCCGAAGAGGGCATCGACTTCACGAACATCTACTTCTACACGGTGCTGTTCCACGCGCTGCGCGCGTCGAACAAGATCGCGATCGAGCGCGGGACGCACTTCAAGGGATTCGAGAAGTCCAAGTACGCCTCGGGCGAGTTCTTCGACAAGTACACCGATCAGGTGTGGGAGCCCAAGACCGACAAGGTGCGCCGGCTGTTCGCCGACGCCGATATCCACATCCCCACGCAGGACGACTGGAAGCAGTTGAAGGAGTCGGTGCAGAAGCACGGCATCTACAACCAGAACCTGCAGGCCGTCCCGCCGACGGGGTCGATCTCCTACATCAACCACTCGACGTCGTCGATCCACCCGGTCGCGTCGAAGATCGAGATCCGCAAGGAAGGCAAGATCGGTCGCGTCTACTACCCGGCGCCGTACATGACCAACGACAACCTGGACTACTACCAGGACGCGTATGAGATCGGGTACGAGAAGATCATCGACACCTACGCCGCGGCCACCCAGCACGTGGACCAGGGTCTGAGCCTGACGTTGTTCTTCAAGGACACCGCCACCACGCGTGACGTGAACAAGGCCCAGATCTACGCATGGCGCAAGGGCATCAAGACGCTCTACTACATCCGCCTGCGCCAGATGGCCTTGGAGGGAACCGAAGTCGAGGGTTGCGTCAGCTGCATGCTGTGA

Protein Sequence

VLPGEADYHALNAMLNLYDADGKIQFEKDREAAKQYFLQHVNQNTVFFHSQDEKLDYLIENEYYEREVLDQYSRDFIKSLLDRAYAKKFRFPTFLGAFKYYTSYTLKTFDGKRYLERFEDRVVMVALTLAAGDTELAEKLVDEIIDGRFQPATPTFLNSGKKQRGEPVSCFLLRIEDNMESIGRSINSALQLSKRGGGVALLLSNIREHGAPIKNIENQSSGVIPIMKLLEDSFSYANQLGARQGAGAVYLHAHHPDIYRFLDTKRENADEKIRIKTLSLGVVIPDITFELAKKNEDMYLFSPYDVERVYGVPFADVSVTEKYYEMVDDARIRKTKINAREFFQTLAELQFESGYPYIMFEDTVNRSNPIAGKITHSNLCSEILQVSTPSEFNDDLSYAKVGKDISCNLGSLNIAKAMDSPDFAQTIEVAIRALTAVSDQTHITSVPSIEQGNNDSHAIGLGQMNLHGYLARERIYYGSEEGIDFTNIYFYTVLFHALRASNKIAIERGTHFKGFEKSKYASGEFFDKYTDQVWEPKTDKVRRLFADADIHIPTQDDWKQLKESVQKHGIYNQNLQAVPPTGSISYINHSTSSIHPVASKIEIRKEGKIGRVYYPAPYMTNDNLDYYQDAYEIGYEKIIDTYAAATQHVDQGLSLTLFFKDTATTRDVNKAQIYAWRKGIKTLYYIRLRQMALEGTEVEGCVSCML_ 
Gene not present in the RLC0011 CRISPRi library.

Specific References

General References