Click the tabs above to view different types of data for this gene. The Vulnerability tab shows CRISPRi fitness data.
DNA and Protein Sequence
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DNA Sequence
>ms0408_MSMEG0408_dnaGTGCCCGGCTTGAGCGACCATTCATCGAGTGGGCCAACCGTGATCTTGCCGATTCGCAAACGTGGTCCTTCCCAATAATTCCCGTTCAGCCGGCCCCCGGCGCGGCTGGAGCCGCTGCGGGAACAACATACTCCGGACCGGCCGTCGGTGGCCGCCCCTAGGGTCGGAACGGCGGACGACACGACGCACATAAGCTCGACACGCGTGCCATAGTGTCCGTCGGGGGGAAAGTTGATCTTCCGGCGCGACATGTCCCCGCAAGGTCACATCGTCGCCGCCGATTCTAGGGAGGACAACGGCATGGTCTCTGCCGAACATCCGATCGAGCCGACCCCGAGCTTCGCAATCATCGGCTACGCCGCACGTTTTCCCGGCGCGGCGAGCGCCGAGGAATACTGGGCGTTGTTGCGGGACGGGCGTGAAGCGATATCCGATGTTCCGAAAGACCGCTGGGACATCGAGGAGTTCTTCGACCCGGACCCGTCGACACCGGGCAAGGTCGTCACGCGCCGCGCGGGGTTCGTCGATGACGTAACGGGTTTCGACGCGCCGTTCTTCGGTATGTCCGCCCGCGAGGTACGTCTGATGGACCCGCAGCACCGGATTCTCATGGAAACCGCGTGGCGCGCGGTCGAACACTCGGGAATCGCGCCGACGTCGCTGGCCAACAGCAACACGGGCGTCTTCGTCGGCCTGGCCACCCACGACTACCTCGGTATGGCCTCCGACGAACTCACCTACCCCGAGATCGAGGCCTACATGGCCATCGGAACCTCCAATGCCGCTGCGGCCGGACGGATCAGTTACCGCCTGGGATTGCAGGGACCGGCGGTGGCGGTCGACACCGCGTGCAGTTCGTCGCTGGTGGCGATTCACCAGGCGTGTCAGGCACTTCGCCTGAACGAGTGCGATCTCGCGTTGGCCGGCGGGGCGAACGTCCTGCTCACCCCGGCCACCATGATCACGTTCTCCAACGCGCACATGCTGGCTCCGGACGGCCGGTGCAAGACCTTCGACGCGGCGGCCGACGGCTACGTGCGCGGCGAGGGCTGCGGTGTCGTCGTGGTCAAGCGCCTCGAGGACGCGGTGCGCGACGGCGACCGGATCCGCGCGGTGATCCGCGGAAGCGCAATCAACCAGGACGGGGCGTCGGGCGGGCTGACCGTGCCGAACGGCGTTGCTCAGCAACGGGTTATCGCCGACGCGTTGAAGCGCGCCGGCCTGCGACCGGCCGATGTCGGTTATCTCGAGGCGCACGGCACCGGAACCTCACTTGGCGACCCCATCGAGGCCCAGGCCGCGGGCGAGGTGCTCGGGGCCGGACGCGCACCTGAAGAACCGCTGCTGATCGGCTCGGCGAAGACCAACATCGGCCATCTGGAAGCGGCCGCGGGCATCGCCGGTGTCATCAAGGTGGTCATGGCGCTCGAGAACGAGACGTTGCCCAAGCACCTCAATTTCGAGACTCCGTCGCCCCACATCCCGTGGGAGCGTCTACCCATCGAGGTGGTGAGAGAGACCGTTCCCTGGCAGCGCAACGGCAAGCCGCGGATCGCCGGGGTGAGCTCATTCGGGTTTGCCGGGACTAACGCGCACGTCATCCTCGAAGAGGCGCCCGCCGTCGGAACACCGGCGACGACAGAGCCTGTCGAGGATCCGAGGCGCTACAGCGTCCTTCCGCTGTCGGCACGCACGCCCGAGGCGTTGGTCCGACTCGCCGGTGAGTACCGCGACTGGCTGCGCGCCCACCCGCAGGCCCGCCTGGCCGACGTGTCCCACACCGCGGGAACCGCACGTGCCCACCTGGAGCAGCGTGCCGCGTTGGTGGTCAACTCGCGGGAATCGGCCATCGAACTGCTCGGCGCGCTCGCCGACGACCGTCCGGCGCCCGGCCTGGTCCGCGGTGAATCCCACGAGGCACCCAAGACCGCGTGGTTGTTCACCGGCCAGGGCAGCCAGTACCCCGGAATGGCGCGTGAGTTGTTCGACACCGAACCGGTGTTCGCCGAAACCGTGAGGAAATGCGCCGAGGCCGTCGCAGATATCCTCGAAAAGCCTTTGCTCGACGTCATTTTCGACGCCGACGAGTTGGATGGTGACACCGAATCGCCGCTGCGGCACACGACCTATGCGCAACCCGCGCTGTTCGCCGTCGAATTGGGCCTGGCCCGGCTCTGGCAGTCCTGGGGCTTCGAACCCGACGTGGTGGTCGGACACAGTGTCGGGCAGTACTCGGCCGCCTGTGTGGCGGGTGTGTTCGACGTCGAGGACGGCGCACGACTGATCGCCGAGCGCGGCCGATTGTTCGGCAGCCTGCCCGCGGGTGGACGCATGGCGGCGGTTTTCACCGCCCCCGAGCGTGCAGAGCGCCTCACCGACGAGTACCCCAGCCTGTCGGTTGCCGCGTACAACGGTGCCAACACCGTATTGTCCGGTCCCGCAAAGGATCTCGAATCCGCCGTGGCCACATTGGTGGCCGACGGTGTGCGGTGCGACTGGCTGGACACCAGCCACGCCTTCCACTCGGCACTGCTCGACCCGATTCTCGACGATTTCGAGTCGTTCGCCCAGAAGCTCGAATACCGTGAACCGCAACGTATCCTGATCGACAACCGCACCGGGTCCGCGCTCGGGCGGAGCACCAGGCTCGACGGTGCCTACTGGCGGCGGCACGCACGCCAGCCTGTCGAGTTCGCCAAGAGCGTCCGCACCCTCGCCGACATGAACTGCAGGGTGCTGCTGGAGATAGGGCCCAGGCCCGTGCTCACCGCCACCGCGCTGGCAGCATGGCCCGACCCGGCCACCACGCCGCAGGTGCTCGCGTCGCTGCGGCCCACCACGGCCGATCACCGGCAGATCACCGAGGCCGTCGCCGCCGCCTATGCCGCGGGACATCTGCCCGATTTCCGCGCGTTCCGGCAACCGGACGCGCGCAAACTCGACCTGCCCACATATCCCTTCGAGCATCGCCAGTTCTGGTTCTCGGACAACCGTGCGATCGACCGTGACACCCAGACCGCGAGCGCCGGTTCCGTCACGCCTCACCGCACACAGGCCGTCCGACTTCTCGAAGACGGCCAGATCGAGGAACTCGCCGCCCTCATCGACGGCGAGACCGTCGACGAGCAGACCCTGCGCGTGCTGAACAAGCTCGCGGCGCGCCACAACCAGCAGCGGTCCACACACGTCGACCCTGATGCGCGGTACGAGATCCGTTGGGACGCGATCGCTTCGGCCGCCGCGAGCACGGGCGCGGCAGCCGACTGGATCCTGGTCGGCGACGACACCGCTGCCGTCCTGGAGTTCGCCGATGCGCTCACCGCAGGCGGTCATCGCCACCAGATCGTCGCGCTGCCCGGCTCCGACGCCGACGAGGCCCAGCTGGTCGATACGCTGCGCGCTGCGTCGGCCGGTGAGCTGTACGTCGTGCACATCGCGGCAGGAGACGGTGCCGCTGCCCCGATGCGGGACCTGCTGCGCGTGCAGCACCGAACCCTTGGTGGGCTGCGGCGCCTGTTCCGCGCCGCGAACGACGCGGAACTGCGCAGCCCCATCTGGTTGGTGACACGTGACGGGCAGCGGGTCACCGACACCGACACCGTTGTGCCGGAACAGAGTTGCCTGTGGGGATTCGGCCGCGCGGCGGCCCTGGAACTCCCGCACATCTGGGGTGGCCTCGCCGACCTCTCCGGTGCGGACACCGAGTGGCCGCGGCTCATCGCGCGGATCACGTCACCGCGCGACGGTGAGGACCAGATCGCGCTGCGCGGGGACGCCGTGTACGCACCCCGACTGGTCCGCCGGACCGGCGAGCCGAGCGACACCCCCCTGCCGGTGCGGGAGGACCGCACCTATCTGGTGACGGGCGGTCTCGGGGCGATCGGGCTCGAGGTCGCCGGCTACCTGGCCTCCCACGGTGCCGGTCATGTGGTGCTCACCAGCCGACGGGAACCGGGCGACGACGCGCGCCGGCGCCTCGACGGTCTCCGCGAGCAGTACGCATGTGACGTCCGGGTGATCACCGCCGATGTCGCCGACGCGCACGACGTCGCGCGCCTCATGACCACCGTCGCCGCCGAACTCCCGCCGCTGGCCGGCATCGTGCACGCCGCGGGCGAGATCGGCACCACGCCGCTGAGCGGCCTCGACGACGCCGAGGTGGATCGCGTCTTCGCCGGGAAGGTCTGGGGCGCCTGGCATCTGAGCGAGGCCCTGACAGATCTTCGCACCGATCTCGACTTCTTCGTCAGCACATCGTCGATCGCGTCGGTGTGGGGCGGATTCGGGCAGACCGCCTACGGCGCGGCGAACGCGTTTCTCGACGGTCTGGCGTGGCGACTGCGCGAGCGGGGTGTCACCGCTGTCAGCGTGAACTTCGGGCCGTGGGCCGCGGGCATGGCCGACGCCGAATCACGTGCCCGCCTCGAGCAGCGAGGCGTGCGGACGTTGTCGCCCGCCGAGGCCCTCGCAGGCATGGCCGACGTCGTGGCCGGCCCCGTTCAGGGAGTCGTCGCCAAGATCGACTGGTCACGTTTCCTGCCGCTGTACCAGCAGGCAGGCCGGCGCGCGTTCCTGTCGGAACTCGAAAGCGAATTGCCCGCAGCGGCAACCGGCGCTGCTGCGCCCGCGACGGTGCCCGGGAAACCACCGCTCGTCGAGCAGTTGACCAAAGCGCCTGTGCAGCAACGCAAAAGCCTCATCACGAACTACCTGCGCAACGCGGTCGCCGAGGTCACACGGGTCGACGCCGACGAGATCCGCGATGAGGCCGGGTTCTTCGACCTCGGGATGGACTCCCTGATGGCCGTCGAACTGCGGCGGCGCATCGAGCAGGGTGTCGGCAAGGACATTCCCGTCACGCTCGTGATGGATCATCCGCGGTTGTCCGACGCGGCCGACTACCTGCTGGTCGAGGTACTCGGGTTGGGCGAGCAGACCAACGTGCGGCAGGCGTCGACCGTGACCGCGCGAACCGACGATCCCATCGCGGTCGTCGCGGTGTCCTGCCGCTTCCCCGGCGCACCCGACCCGGAGTCGTTCTGGGAGCTGCTCGCCGGTGGTGTCGATGCCATCCGAGAGGTCCCCGAGGACCGCTGGGACATCGACGAGTTCTACGACCCCGATCCGGACACCCCGGGCAAGACCTACACGCGGTTCGGCGGATTCCTCGACGGCATCGACGGATTCGATCCCGAGTTCTTCGGCATCTCACCGCGTGAGGCCGTGTGGATCGAGCCGCAGCAGCGCCTCATGCTCGAGACGGTGTGGGAGGGTCTCGAGCGGGCCGGCTACGCACCTTCGGACCTCCGGGGCAGCCGCACGGGTGTCTTCACCGGTGTTGCCGCCAACGAGTACGCGCATCTGCTGTCGGCCGAGTCGATCGACAAGATCGAGCCGCACTTCATCACGGGCAATGCGCTCAACGCGATCTCCGGTCGCGTGGCGTTCGCGCTGGGCTTCGAAGGTCCTGCCGTCGCGGTCGACACCGCGTGCAGCTCGGCTCTGGTCGCGGTCCATCAGGCATGTCAGGCACTGCAGTCCGGGGACTGCGATCTGGCCCTCGCGGGAGGCGTCAACGTGCTGCTGAGCCCCGTCACCGTGGTCGCGGCCTCACGCGCGCGGATGCTGTCGCCGGTGGGCCGGTGCAAGACGTTCGACGCGTCCGCCGACGGGTATGTGCGCAGTGAGGGCTGCGGCGTCCTGGTGCTCAAGCGCCTCAGCGACGCCGTGCGTGACGGCGACCGGGTGTGCGCGGTGATCCCTGCGAGCTCGGTGAACCAGGACGGCGCCTCCAGCGGGTTGACCGTCCCCAACGGCGGTGCGCAGCAACGGCTCATCGAGGCGACGCTGGCCCGCGCCGGACTGAGCGGCGCCGATGTGGACTACCTCGAGGCACACGGCACGGGTACGCCCCTCGGTGACCCGATCGAGGTGCAGGCCGCCGCGGCCGCGTACGGCGCCGGGCGTGACGCCGACCGGCCGCTGCTGATGGGATCGGTGAAGACCAACATCGGGCACACCGAATCCGCCTCGGGTGCAGCGGGTCTGATCAAGGTGGTGCTGTCACTGCAACACGGGGTGCTGCCGCAGAGCCTGCACTTCGAGAATCCGTCACCGCACATCCCGTGGGACGCATTGCCGGTGCGGGTGATCGACGAACCGGTGCCGTGGCAGACCAACGGCCGGGTGCGCCGTGCCGGTGTCAGTTCGTTCGGGTTCACCGGCACCAACGCGCACGTGCTCGTCGAGGAGGCCCCGCCGCAACTGCACACCGCCGACGCTGCGCCCGTGGAACCGGCCGCCACCGCGGAGGCCGGGACCGACGACTCGCCGCAGGTGCTGGCGCTGTCGGCGCGGTCGCCCGAGGCGCTCGTGGAGTTGGCGCGGCGCTACGACGACTGGTTGAGCGCGACGCCCGGCCTCGACATCGCCGACGTGTGCCACACCGCGGGCGTGGGCCGTTCGCACTTCGAATACCGCGCAGCCCTGGTGGTCGACTCGGTCGAGCGGGCACGCGAGGGTCTCGCCGCGCTCGCCGAGGGGCGCACTCCTGCGGGCGTGGTGCGCGGCGAGCATGTGCACCGTCCGACGACGGCATGGCTGTTCACCGGACAGGGCAGCCAGTACCCGGGCATGGCCCGCGAATTGTTCGACACGCAACCGGTTTTCGCCCAGACCGTTCGGCAGTGCGCCGAGGCGGTCGCCGACATGCTGCCGCGACCACTGCTGGACGTGCTGTTCGCGACCGACCGCGAAACCGCGGACCTGCTGCAGCACACGTCGTTCGCGCAGCCCGCGCTGTTCGCGGTCGAGATGGGTCTGGCGCGGCTGTGGCAGTCATGGGCCATCGAACCCGATGTGGTGCTGGGGCACAGCGTCGGCCAGTACGCCGCGGCCTGCGTGGCGGGCGTGTTCAGCCTTGACGACGGCGCACGACTGATGGCCGAGCGCGGCCGCATGTTCGGCAGCCTTCCCGAAGGCGGCCGGATGGTGGCCGTGTTCGCCGACGCCGAACACGTCGAGCAGGTGGCCGGTGAGTTCCCCCGGGTGTCGGTCGGCGCCTACAACGGCCCCAACACCGTGCTCTCGGGTCCCGGCGAGGACCTCGAAGAGATCGTCGAGAGGTTCGAGGACGAGGGGATCCGCTGCACGTGGCTGGCGACCAGCCACGCGTTCCACTCCGAACTGCTCGATCCCGTGCTCGACGAGTTCGAGGCCTACGCGGCCCAGTTCCAGTTCGCAGCGCCGACACTGCCTTTGGTGTGCAACCGGACCGGGGCCGTGCTCACCGGGCAGACCCCGCTCGACGCGCAGTACTGGCGCAGGCATTCCCGCCAGCCCGTGCAGTTCGCCGAGAGTGTGCGCACCGTCGCCGCGCTCGGCTGCTCGGTGCTGATGGAGATCGGCCCGCAACCTGTGCTGACCGGGGCCGCGGTACAGGTGTGGCCCGAACACATGGCTGCCCCGCGGGCCGTCGCCTCGCTGCGCAAGGGCGTCGCCGATCGCCGACAGATCGCCGATGCGCTGGCGTCGGCCTACGTCGGCGGCCACCGCCCCGACTTCGCCGCGGTGCACCGGCAGCCACGCCGCCCGGTCGAGTTGCCCACCTATCCGTTCCAGCGCCGCAGGTTCTGGCCGAAGGCCTCGAGCATCGCCGTCGACGGTGGGACCGCGGCGTCGGGAATCCTGGGCAGCGGCAAGGATCTGGCGTCCGGCGACACCATCTACACGAGCCGGCTGTCCGTCAAATCGCAGCCGTGGCTGGCCGATCACGTCATCTACGGCACCGTCGTCGTCCCCGGCGCGACGTATGCGGCGATGGCGCTCGCCGCGGTCGGCACACCGGCACGCCTGAAGGACGTGTTCTTCTACGAGCCGATCATCCTGCCCGAGAAGGCCTCTCGCGAGGTCCAGCTGACGTTGCACCCTGCCGACGGTGGCAGCGTGCTCAGCTTCCAGATCCACAGCCGCCCGTACGGTGAACGTGGTGCCGACTGGTCGCTGAACGCCGAGGGCACGGTCGACACGGCCGGCAGCGACCCGGACGCCGCCGCAGACGAGCAGTCGGATCCGGTCGACGAGGTGATCGAACGCCTCGAGCGCATGCGTCCGCAGGACCTCTTCGAGACCTTCGCCGACCTGGAACTGGCGTGGGGTCCCACATGGTCGGGCTCGCTGAAATCGTTGTGGCTCGGCGACGGTGAGGCCGTCGGCGACATCCTCGTCGGCGCCGAACTCGCCGAGCAACTCGGCTCCGAGCCCATGCACCCGGTGCTGATGGACCTGTGCACGGGCGTCGCGTTCCCCGCGTTCCCGGCGCTGCTCGCGGCCGAACAGGGCGTCAACGACCTGTTCCTGCCGCTGCGCTACGGGCAGGTGACGCTCGCGGAGAAGATGCCGCGGCGGTTCTACTGCCGGGCGAGGTGGCACCGTAGCGCGCTCGACAGCGAAACGCAGGTGTTCGAACTCGAATTCGTCGACCTCGGTGGGCGAGCGCTCGGCGGCATCCGCGAGTTCACCGTCAAACGTGCGCCGCGCGAGGCACTGCTGCGCGGCCTCGGGGGTGACGCGACCCGCCTGCTCTACACGCTCGGGTGGCACGAGGTGCCGCCGCCACCATCGGCCGACGACGCGGTCGGCACGTGGCTGATCGCCGGGTTCGACGAGCTGGCCGCGAAGGTGCCCGGCTGTGTCCCCATCGACCGCAACACCGATCTGCAGGTCCTCGGTGAGGTGCTGTCGCAGGCGCACGCTCGCGGCGCGGGATTCTCGGGCGTCGTGTGGCGTTGCGCGACGCCTGGCCGGAAGGAATCGGGTTCTGCCTCCGCCGAATCCGCCCGTCTGGAGGCCGAGATCACCAATCTGCTCAGCGCGGTGCACGCCGCGCAGGGGCCGAGCGTGAAACTGCCCGGCGGACTCTGGATCGTCACCGAACACGGCGTGGCATGTGAATCCGGCGAGCCGGTGGACCCGGTGCAGGCCGCGCTGTGGGGCTTCGGCCGAACGACGATCAACGAGGAACCGGCGCTGCACTGCCGGCTCGTCGACTGCGACGGATCTTCCGAGGCCGTCGACCTGCTGGCCGCTCTGCTGGCCGCACCGGGCATCCAGGAACCGGAAATCGCCGTGCGGCAGGGCAAACTGCTGGCGTCACGGTTGTTGCCGTGGGCCCGCAGTGGTCATCTCACGCTGCCGCGCGGGGGCGACTTCGTCCTTGCGCCCACCGAGCGCGGCGCGATCGACAACCTGCGCCTCACGGAGACGGACGTGCCGGAGCCGGACGAGGGCTACGTGCAGGTTCGGGTCGAGGCCGCGGGCCTCAACTTCCGCGACGTGCTCAACGTGCTCGGCCTGTACCCCGGTGATCCGGGACCGATCGGCGGCGACTTCGCAGGCACCGTGACCCAACTGGGGGCAGGTGTGAGTGGACTCGAAATCGGCCAACGCGTGTACGGGTCGATGCAGGGTGCCTTCGCGAGCCGCTTCAACGTGCCCGCCCAGTTCCTGGCGCCGATTCCCGACGGCATCGGCGCGGTCGAGGCCGCCACGATCCCGGCCGCGGCGCTGACGGTCCGGCTCGCGTTCGACTGGGCGCAACTCAAACCCGGCGACAAGGTGCTCATCCACGCCGCCAGCGGTGGCGTGGGGCTCGCGGCCATCCAGATGGCGCAGCAGTCCGGCGCCGAGGTCTTCGCCACCGCGAGCACCTTCAAGCGTTCGACCCTGCGCAGGCTGGGCGTGAAGTACGTCTACGACTCTCGGACGACGGACTTCGCCGACCAGATCCTCGCGGACACCAACGGTGCCGGCGTGGACGTGGTGCTCAACAGCCTCACGAGCGCGGGTTTCGTCGAGGCGACGCTGAAGGCCACCGCGCAGGGCGGACGCTTCGCCGAGATCGCCAAGCGCGACATCTGGTCGCATGAGCAGATGGCCGAGGCCCGCCCCGACATCGCCTACGAGATCGTCGCGCTGGACACCGTGATGTTCACCGAGCCCGATCGCATCCGCGACCTGCTCACCGAGGTGTCCGACGGGATGGCGCGCGGCGAGTGGACACCACTACCCGCCGAGATCTACCCGATCACCGAGGCCCGGTCGGCGTTCCGGCGCATGCAACAGGCACGGCACATCGGCAAGATTGTGCTGCAGGTCCCCAAACCCCTTCAGCCGCAGCAGGACCGGAGCTACCTGATCACCGGCGGACTCGGGGCGATCGGCCTGCACACGGCGGCGTATCTGGCGCAGCTCGGCGCGGGCGACATCGTGCTGACCAGCCGGCGCGACCCCGACGAGTCGGCCCAGCGGGCGATCGACGAGATCACCGAGCGCCACAAGTGCCGCATCCACACCTATGCGGCCGATGTGGGTGACGAGGCACAGGTGGCCGATCTGCTGGCGCGGATCCGGGCCGAGTTGCCTCCACTGGCCGGAATCGCCCATCTGGCGGGCGTGCTCGACGACGCGCTGCTGTCGGCGCAGAACCCCGAGCGCTTCCGGACCACGTTGGCGCCCAAGGCCTATGGCGCGAGCCACCTGGACCGCCTGACGCGCGACGACGACCTGGACTTCTTCATCGTGTCCTCGTCGGTGTCGAGCCTGTTCGGATCACCGGGGCAGGCCAACTACGCAACCGCCAATGCGATGCTCGACGGTCTGGTCGCCAAGCGCCGTGCGCAGGGCCTGGTGGCCACGGGCATCAACTTCGGGCCGTGGGGTCAGGGCGGCATGGCGTCGTCGGAGGCCGCGACCGCGAACATCACCGCCCAAGGTCTGATCCCGTTGGATCCGTCGGCCGCGTTGCACGCGCTGGCCGAGGTCATCGCGAACGGCTCCGGGCAGGCGACGGTGCTCAAGGCCAACTGGCAGCGCGCGGCGAAGGTTCTCGGCTCGGCGCGCCCGCCGATCCTGGACCTCGTCCTGCCGAGCGCGGCCGGGGAGGCCACGGGTGACAGCGAGCTGCTCAAGCAGTTGATGGAGATCCCGATACCGCAGCGCGCCGGGTTCGTCACCGAGTTCCTGCAACGCGAAGTGCAGAACTTCCTGCGTCTCGCGTCGCCGCCCGCGGCGTCGAGCCGGTTCCTGGACCTCGGCACGGATTCGCTGATGGCCATCGAGCTGCGCAACCGGCTGCACAGCCAGTTCGGGGGCAAGTTCACCATCAACGCGACCGCGGTGTTCGACTACCCGACCATCGGCGGGCTCGCGGAGTACCTCGTGGGTCAGCTGCCCGACGCCGAGGCGCCCGAAACGGCCTGA
Protein Sequence
>Ms0408_MSMEG0408_proteinMVSAEHPIEPTPSFAIIGYAARFPGAASAEEYWALLRDGREAISDVPKDRWDIEEFFDPDPSTPGKVVTRRAGFVDDVTGFDAPFFGMSAREVRLMDPQHRILMETAWRAVEHSGIAPTSLANSNTGVFVGLATHDYLGMASDELTYPEIEAYMAIGTSNAAAAGRISYRLGLQGPAVAVDTACSSSLVAIHQACQALRLNECDLALAGGANVLLTPATMITFSNAHMLAPDGRCKTFDAAADGYVRGEGCGVVVVKRLEDAVRDGDRIRAVIRGSAINQDGASGGLTVPNGVAQQRVIADALKRAGLRPADVGYLEAHGTGTSLGDPIEAQAAGEVLGAGRAPEEPLLIGSAKTNIGHLEAAAGIAGVIKVVMALENETLPKHLNFETPSPHIPWERLPIEVVRETVPWQRNGKPRIAGVSSFGFAGTNAHVILEEAPAVGTPATTEPVEDPRRYSVLPLSARTPEALVRLAGEYRDWLRAHPQARLADVSHTAGTARAHLEQRAALVVNSRESAIELLGALADDRPAPGLVRGESHEAPKTAWLFTGQGSQYPGMARELFDTEPVFAETVRKCAEAVADILEKPLLDVIFDADELDGDTESPLRHTTYAQPALFAVELGLARLWQSWGFEPDVVVGHSVGQYSAACVAGVFDVEDGARLIAERGRLFGSLPAGGRMAAVFTAPERAERLTDEYPSLSVAAYNGANTVLSGPAKDLESAVATLVADGVRCDWLDTSHAFHSALLDPILDDFESFAQKLEYREPQRILIDNRTGSALGRSTRLDGAYWRRHARQPVEFAKSVRTLADMNCRVLLEIGPRPVLTATALAAWPDPATTPQVLASLRPTTADHRQITEAVAAAYAAGHLPDFRAFRQPDARKLDLPTYPFEHRQFWFSDNRAIDRDTQTASAGSVTPHRTQAVRLLEDGQIEELAALIDGETVDEQTLRVLNKLAARHNQQRSTHVDPDARYEIRWDAIASAAASTGAAADWILVGDDTAAVLEFADALTAGGHRHQIVALPGSDADEAQLVDTLRAASAGELYVVHIAAGDGAAAPMRDLLRVQHRTLGGLRRLFRAANDAELRSPIWLVTRDGQRVTDTDTVVPEQSCLWGFGRAAALELPHIWGGLADLSGADTEWPRLIARITSPRDGEDQIALRGDAVYAPRLVRRTGEPSDTPLPVREDRTYLVTGGLGAIGLEVAGYLASHGAGHVVLTSRREPGDDARRRLDGLREQYACDVRVITADVADAHDVARLMTTVAAELPPLAGIVHAAGEIGTTPLSGLDDAEVDRVFAGKVWGAWHLSEALTDLRTDLDFFVSTSSIASVWGGFGQTAYGAANAFLDGLAWRLRERGVTAVSVNFGPWAAGMADAESRARLEQRGVRTLSPAEALAGMADVVAGPVQGVVAKIDWSRFLPLYQQAGRRAFLSELESELPAAATGAAAPATVPGKPPLVEQLTKAPVQQRKSLITNYLRNAVAEVTRVDADEIRDEAGFFDLGMDSLMAVELRRRIEQGVGKDIPVTLVMDHPRLSDAADYLLVEVLGLGEQTNVRQASTVTARTDDPIAVVAVSCRFPGAPDPESFWELLAGGVDAIREVPEDRWDIDEFYDPDPDTPGKTYTRFGGFLDGIDGFDPEFFGISPREAVWIEPQQRLMLETVWEGLERAGYAPSDLRGSRTGVFTGVAANEYAHLLSAESIDKIEPHFITGNALNAISGRVAFALGFEGPAVAVDTACSSALVAVHQACQALQSGDCDLALAGGVNVLLSPVTVVAASRARMLSPVGRCKTFDASADGYVRSEGCGVLVLKRLSDAVRDGDRVCAVIPASSVNQDGASSGLTVPNGGAQQRLIEATLARAGLSGADVDYLEAHGTGTPLGDPIEVQAAAAAYGAGRDADRPLLMGSVKTNIGHTESASGAAGLIKVVLSLQHGVLPQSLHFENPSPHIPWDALPVRVIDEPVPWQTNGRVRRAGVSSFGFTGTNAHVLVEEAPPQLHTADAAPVEPAATAEAGTDDSPQVLALSARSPEALVELARRYDDWLSATPGLDIADVCHTAGVGRSHFEYRAALVVDSVERAREGLAALAEGRTPAGVVRGEHVHRPTTAWLFTGQGSQYPGMARELFDTQPVFAQTVRQCAEAVADMLPRPLLDVLFATDRETADLLQHTSFAQPALFAVEMGLARLWQSWAIEPDVVLGHSVGQYAAACVAGVFSLDDGARLMAERGRMFGSLPEGGRMVAVFADAEHVEQVAGEFPRVSVGAYNGPNTVLSGPGEDLEEIVERFEDEGIRCTWLATSHAFHSELLDPVLDEFEAYAAQFQFAAPTLPLVCNRTGAVLTGQTPLDAQYWRRHSRQPVQFAESVRTVAALGCSVLMEIGPQPVLTGAAVQVWPEHMAAPRAVASLRKGVADRRQIADALASAYVGGHRPDFAAVHRQPRRPVELPTYPFQRRRFWPKASSIAVDGGTAASGILGSGKDLASGDTIYTSRLSVKSQPWLADHVIYGTVVVPGATYAAMALAAVGTPARLKDVFFYEPIILPEKASREVQLTLHPADGGSVLSFQIHSRPYGERGADWSLNAEGTVDTAGSDPDAAADEQSDPVDEVIERLERMRPQDLFETFADLELAWGPTWSGSLKSLWLGDGEAVGDILVGAELAEQLGSEPMHPVLMDLCTGVAFPAFPALLAAEQGVNDLFLPLRYGQVTLAEKMPRRFYCRARWHRSALDSETQVFELEFVDLGGRALGGIREFTVKRAPREALLRGLGGDATRLLYTLGWHEVPPPPSADDAVGTWLIAGFDELAAKVPGCVPIDRNTDLQVLGEVLSQAHARGAGFSGVVWRCATPGRKESGSASAESARLEAEITNLLSAVHAAQGPSVKLPGGLWIVTEHGVACESGEPVDPVQAALWGFGRTTINEEPALHCRLVDCDGSSEAVDLLAALLAAPGIQEPEIAVRQGKLLASRLLPWARSGHLTLPRGGDFVLAPTERGAIDNLRLTETDVPEPDEGYVQVRVEAAGLNFRDVLNVLGLYPGDPGPIGGDFAGTVTQLGAGVSGLEIGQRVYGSMQGAFASRFNVPAQFLAPIPDGIGAVEAATIPAAALTVRLAFDWAQLKPGDKVLIHAASGGVGLAAIQMAQQSGAEVFATASTFKRSTLRRLGVKYVYDSRTTDFADQILADTNGAGVDVVLNSLTSAGFVEATLKATAQGGRFAEIAKRDIWSHEQMAEARPDIAYEIVALDTVMFTEPDRIRDLLTEVSDGMARGEWTPLPAEIYPITEARSAFRRMQQARHIGKIVLQVPKPLQPQQDRSYLITGGLGAIGLHTAAYLAQLGAGDIVLTSRRDPDESAQRAIDEITERHKCRIHTYAADVGDEAQVADLLARIRAELPPLAGIAHLAGVLDDALLSAQNPERFRTTLAPKAYGASHLDRLTRDDDLDFFIVSSSVSSLFGSPGQANYATANAMLDGLVAKRRAQGLVATGINFGPWGQGGMASSEAATANITAQGLIPLDPSAALHALAEVIANGSGQATVLKANWQRAAKVLGSARPPILDLVLPSAAGEATGDSELLKQLMEIPIPQRAGFVTEFLQREVQNFLRLASPPAASSRFLDLGTDSLMAIELRNRLHSQFGGKFTINATAVFDYPTIGGLAEYLVGQLPDAEAPETA
Essentiality:
| Call | Condition | Type | Source | Notes |
|---|---|---|---|---|
| Non Essential | in vitro | CRISPRi | [Bosch & DeJesus et al. (2021)] | |
| Non Essential | in vitro | TnSeq | [Dragset et al. (2019)] |
Vulnerability Assessment
help
[Full list of passaging experiments]
Experiment (PMID: 34297925): We designed an M. tuberculosis CRISPRi library (RLC12) to target all annotated Mtb genes with single guide RNAs (sgRNAs) of varying predicted knockdown efficiencies.
In parallel, we constructed a M. smegmatis CRISPRi library (RLC11) to target all annotated Msmeg genes, similar to the Mtb sgRNA library.
RLC12 was transformed into M. tuberculosis H37Rv; RLC11 into M. smegmatis mc2-155.
We passaged both CRISPRi libraries for nearly 30 generations in the presence or absence of the CRISPRi-inducer anhydrotetracycline (ATc). At the indicated timepoints, genomic DNA was harvested and the representation of individual sgRNAs was counted using Illumina sequencing.
Here, we show sgRNA log2 fold-change values (L2FC) (plus versus minus ATc) over consecutive generations for sgRNAs targeting the gene you selected.
sgRNAs are color-coded based on their predicted strength from blue (strength=0; weak) to red (strength=1; strong).
In order to quantify gene vulnerability, data were subsequently analyzed with a hierarchical model at the sgRNA level (two-line model) and gene level (Hill curve). The vulnerability quantification is shown below. [More information]
| M. smegmatis Reference Fitness Experiment | M. tuberculosis H37Rv Reference Fitness Experiment | M. tuberculosis HN878 Reference Fitness Experiment | |
|---|---|---|---|
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| M. smegmatis Gene-Level Logistic Regression | M. tuberculosis H37Rv Gene-Level Logistic Regression (Most similar ortholog) | M. tuberculosis HN878 Gene-Level Logistic Regression (Most similar ortholog) | |
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| Vulnerability Index (VI) | 0.1880 | 1.1340 | |
| VI Lower Bound | 0.1500 | -0.8600 | |
| VI Upper Bound | 0.2260 | 4.0530 |
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