MSMEG4155
ms4155
-
M. smegmatis · MC2-155
[AlphaFold] [Orthologs] [Phyre2] [UniProt] [MycoBrowser] [TnSeq DB] [SeqHub]
MC2-155 Coordinates: 4234097 – 4235137
Vulnerability Index (VI): 0.0520
M. tuberculosis homologs Rv2309c, Rv2646
M. abscessus homologs MAB0442, MAB4875c
background  +5   0  -5 -10 -15 sgRNA L2FC (+ATc/–ATc) TnSeq Gene Essentiality Predictions (1, 2) Essential Growth Defect Non-Essential Growth Advantage Unknown Operon Legend (3) Operon (1) PMID: 28096490 (2) PMID: 31388080 (3) PMID: 26536359 MSMEG4150 MSMEG4151 MSMEG4152 MSMEG4153 MSMEG4154 MSMEG4155 MSMEG4156 MSMEG4157 MSMEG4158
Click the tabs above to view different types of data for this gene. The Vulnerability tab shows CRISPRi fitness data.
DNA and Protein Sequence help

DNA Sequence 

>ms4155_MSMEG4155_dna
GAGCTTCCCGAAAGTGGCCGCGATGATGGACGAGGCGAAAGCCGACGTGCTGGCGTTCACCGCGTTCCCGCGCACTCACTGGCAGAAGATCTGGTCGAACAACCCCATCGTTATCTGTAGTGCTGCGGTGAGTGTCTTTTATGTGGACCACGAAGGCTCAGAGTGGTCGGTTCTGCGTTCTGGATGTGGTTATCTCCAGCCGTCCATGAAGCAGATCCTGGAGGGTCGATGTGCTTGTTGTGAAGGTGGTTTCACCGCTCTCGTCGCGTGAATCGTTCACCGTCCTCGGTGAGGACGACGTGCCGGTGGCACCGGTGGAGCGGTATCTGAAGTATTTGACCGACATCGAGCGGTCGCCGAACACAATCAAGGCGTACGCCCATGATCTGAAGGACTGGTTTACATTCCTGGGCGGGCGCGGCCTGGACTGGCAGTCGGTGAGCCTGGAGGATATCGGCGCGTTCGTCGTCTGGCTGCGACTGCCGACGGCACTGCGGCAGGGCGGGATCGCCGTGCTGCCCTCGGTGGAGCATCACTGCGGCGAGTCGACGGTGAACCGGAAGCTGTCGGCGTTGGCGGCGTTCTATCTGCACGCCGTCCGCGACGGGATCGATGTCGGTGAGCTGCTGACGACCTGGCAGATCGGTGGGTCGAGAGGCGGTTGGAAGCCGTTTCTGCACCACATCAGCAAGCACATGCCGAGCCCCCGGCGAACGGTGTCGTTGAAGGCGCCGAAGAAGCTGCCGCGGGTGCTGACCCCCGGCGAGGTCCAGACACTGCTGGATGCGTGCGATCGGTTGCGGGATCGGTTCCTATTGGCGCTGCTCTATGACGCCGGGATGCGGATCGGTGAGGCGCTGGGGTTGCGTCACAGCGACATTGCCGCCGCTGAACGGCAGGTCACAGTTTGTCGGCGTGACAACGACAACCGAGCCCGCGCCAAATCGTTGACGGCCCGGATCGTTCCGGTGAGCGCGGAACTGATTCGGTTGTATGCCGATTACCTTCATGCCGAATACGGTGACCTCGACAGTGACTACGTTTTCGTCAACCTCTGGGGGCGGCCCCACGGCCATCCGTTGACCTATGGCGCGGTCTATGACTTGGTCCGCCGATTGCGCCGGCGCACGGCCATCGACTTCGACCCGCATTGGCTGCGTCACACCGCCGCGACCAGGATGCTGCGCGACGGGATCGGCTTGGAGGTCGTGGCCAAGCTGCTCGGCCACGCCAATGTCACCGTCACCGCCGCGACATATGGGCATCTGAACGTGGAGGACGCCCGGAAGGCCATGGAGCAAGCGGGCTGGTTCATCGGAAAGGCCCAGGTCAAACTGTGA

Protein Sequence 

>Ms4155_MSMEG4155_protein
VPVAPVERYLKYLTDIERSPNTIKAYAHDLKDWFTFLGGRGLDWQSVSLEDIGAFVVWLRLPTALRQGGIAVLPSVEHHCGESTVNRKLSALAAFYLHAVRDGIDVGELLTTWQIGGSRGGWKPFLHHISKHMPSPRRTVSLKAPKKLPRVLTPGEVQTLLDACDRLRDRFLLALLYDAGMRIGEALGLRHSDIAAAERQVTVCRRDNDNRARAKSLTARIVPVSAELIRLYADYLHAEYGDLDSDYVFVNLWGRPHGHPLTYGAVYDLVRRLRRRTAIDFDPHWLRHTAATRMLRDGIGLEVVAKLLGHANVTVTAATYGHLNVEDARKAMEQAGWFIGKAQVKL

Essentiality:

Call Condition Type Source Notes
Non Essential in vitro CRISPRi [Bosch & DeJesus et al. (2021)]
Non Essential in vitro TnSeq [Dragset et al. (2019)]
Vulnerability Assessment help
[Full list of passaging experiments]

Experiment (PMID: 34297925): We designed an M. tuberculosis CRISPRi library (RLC12) to target all annotated Mtb genes with single guide RNAs (sgRNAs) of varying predicted knockdown efficiencies. In parallel, we constructed a M. smegmatis CRISPRi library (RLC11) to target all annotated Msmeg genes, similar to the Mtb sgRNA library. RLC12 was transformed into M. tuberculosis H37Rv; RLC11 into M. smegmatis mc2-155. We passaged both CRISPRi libraries for nearly 30 generations in the presence or absence of the CRISPRi-inducer anhydrotetracycline (ATc). At the indicated timepoints, genomic DNA was harvested and the representation of individual sgRNAs was counted using Illumina sequencing. Here, we show sgRNA log2 fold-change values (L2FC) (plus versus minus ATc) over consecutive generations for sgRNAs targeting the gene you selected. sgRNAs are color-coded based on their predicted strength from blue (strength=0; weak) to red (strength=1; strong).

In order to quantify gene vulnerability, data were subsequently analyzed with a hierarchical model at the sgRNA level (two-line model) and gene level (Hill curve). The vulnerability quantification is shown below. [More information]

M. smegmatis Reference Fitness Experiment M. tuberculosis H37Rv Reference Fitness Experiment M. tuberculosis HN878 Reference Fitness Experiment
 
 
M. smegmatis Gene-Level Logistic Regression M. tuberculosis H37Rv Gene-Level Logistic Regression (Most similar ortholog) M. tuberculosis HN878 Gene-Level Logistic Regression (Most similar ortholog)
 
Vulnerability Index (VI) 0.0520 -7.7170
VI Lower Bound -0.2750 -16.4290
VI Upper Bound 0.5080 1.6710

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