MSMEG2291 (MSMEG2291)

background  +5   0  -5 -10 -15 sgRNA L2FC (+ATc/–ATc) TnSeq Gene Essentiality Predictions (1, 2) Essential Growth Defect Non-Essential Growth Advantage Unknown Operon Legend (3) Operon (1) PMID: 28096490 (2) PMID: 31388080 (3) PMID: 26536359 MSMEG2288 MSMEG2289 MSMEG2290 MSMEG2291 MSMEG2292 MSMEG2293 dinB
Species: M. smegmatis
Strain: MC2-155
PATRIC Annotation: -
Synonyms:
M. tuberculosis homologs RVBD3057c, RVBD0851c, RVBD3085, RVBD2766c, RVBD0765c, [+ 17 remaining orthologs]
M. abscessus homologs MAB3420c, MAB4680c, MAB0090c, MAB3919c, MAB4019c, [+ 37 remaining orthologs]
Gene Start: 2373847
Gene End: 2374710
Vulnerability Index (VI):
VI Lower Bound:
VI Upper Bound:
[AlphaFold] [Orthologs] [Phyre2] [UniProt] [MycoBrowser] [TnSeq DB]

Essentiality:

Call Condition Type Source Notes
Growth Advantage in vitro TnSeq [Akusobi et al. (2022)]
Unknown in vitro CRISPRi [Bosch & DeJesus et al. (2021)]


DNA and Protein Sequence help

DNA Sequence

ACCGTCGCGCAGAACACCGACGCGCAGATCCGCGACATCATGAACGACTTCGAGGACGCCACGATCGCCAAGATCGTCGGCGTCATCGAACAGGACACCGGAGCCCGGCCGATCTCCGACGATCTGCCCGCACTGGTGCGCACCCTGACCGCGACCACGGCGATGACACTGTCGCGTGACGCCGGGTTCGTGGGCCGTGACGCCGATCCCGCGCGTGCCGTCGAGATCGTCGAACGCCTGTGGCTCAGCGCGCTGTGGGGTGGCCTGCCGGAGTAGATCTCCCCGGTAAGGTCACCGCTATGGCGCAGGGGAGCGACTTCGCGGGCAAGAGATGTTTCGTGACCGGGGCGGCCAGCGGCATCGGCCGCGCCACGGCGCTGGCGCTCGCCGCGGCGGGCGCCGAGCTCTACCTGACCGACCGCGACGCCGACGGGCTTGTGCAGACCGTCGCCGATGCGCGCGCGCTCGGCGCCGAGGTGCCTGCGCACCGCGCGCTCGACATCTCCGACTACGACCAAGTGCGCGAGTTCGCCAAGGACATCCACGCCGCGCACGGGTCGATGGACGTCGTGATGAACATCGCGGGCGTGTCCGCGTGGGGCACCGTCGACCGGCTCAGTCACCAGCAGTGGCGGTCGATGGTCGACATCAACCTCATGGGGCCCATCCACGTCATCGAGGAGTTCATCCCGCCGATGATCGAGGCGCGCCGCGGCGGGCACCTGGTCAACGTGTCCTCGGCGGCCGGTCTGGTCGCCCTGCCGTGGCACGGTGCCTACAGCGCAAGCAAATTCGGCCTGCGCGGCGTGAGCGAGGTGCTGCGATTCGACCTGGCGCGACACCGCATCGGGGTGTCGGTGGTGGTGCCCGGCGCCGTGAAGACCGGCCTGGTGCAGACCGTCGAGATCGCCGGCGTCGACCGCGAGGACCCCGACGTCAAGAAGTGGGTGGATCGCTTTGCCGGACATGCGATCTCACCTGAGAAGGCCGCCGCGAAGATCCTGGCGGGGGTACGACGCAACCGGTTTTTGATCTACACCTCGGCCGACATCCGCGCGCTGTACACGTTCAAGCGCGTGGCCTGGTGGCCCTACAGCGTGGCGATGCGCCAGGTGAACGTGCTGTTCACCCGCGCTCTGCGCTCCGGCACATCGCCACGCTGA

Protein Sequence

MAQGSDFAGKRCFVTGAASGIGRATALALAAAGAELYLTDRDADGLVQTVADARALGAEVPAHRALDISDYDQVREFAKDIHAAHGSMDVVMNIAGVSAWGTVDRLSHQQWRSMVDINLMGPIHVIEEFIPPMIEARRGGHLVNVSSAAGLVALPWHGAYSASKFGLRGVSEVLRFDLARHRIGVSVVVPGAVKTGLVQTVEIAGVDREDPDVKKWVDRFAGHAISPEKAAAKILAGVRRNRFLIYTSADIRALYTFKRVAWWPYSVAMRQVNVLFTRALRSGTSPR_ 
Gene not present in the RLC0011 CRISPRi library.

Specific References

General References